Person: MERAL, İSMAİL
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Publication Open Access Effects of Cichorium Intybus on GABAA Receptors and Apoptosis in Pentyleneterazole-PTZ Kindling in Rats.(2021-08-01T00:00:00Z) Meral, İsmail; Eşrefoğlu, Mukaddes; Kara, Mehmet; Erkeç, Özlem Ergül; Tok, Olgu Enis; Armağan, Metin; MERAL, İSMAİL; EŞREFOĞLU, MUKADDES; ÜSTÜNOVA, SAVAŞPublication Metadata only Sulodexide protected the heart against ischemia/reperfusion injury by reducing oxidative stress, inflammation, and apoptosis in the isolated rat heart(2022-11-17) ÜSTÜNOVA S.; AKSOY R.; BULUT H.; KILIÇ A.; MERAL İ.; RABUŞ M. B.; BOZKURT A. K.; KÖKSAL C.; ÜSTÜNOVA, SAVAŞ; KILIÇ, AYSU; MERAL, İSMAİL; KÖKSAL, CENGİZPublication Open Access Angiotensin IV improves spatial memory in streptozotocin-induced diabetic rats by reducing oxidative stress and altering BDNF levels(2021-01-01T00:00:00Z) KILIÇ, AYSU; ÜSTÜNOVA, SAVAŞ; ELİBOL, BİRSEN; Bulut, Huri; MERAL, İSMAİL; Sahin, Gulderen; KILIÇ, AYSU; ÜSTÜNOVA, SAVAŞ; ELİBOL, BİRSEN; MERAL, İSMAİLIn this study, we investigated the protective effects of angiotensin IV (Mg IV) on cognitive function in streptozotocin (STZ)-induced diabetic rats. Male Wistar albino rats, were randomly divided into four groups; control (C), diabetes (Dia, 60 mg/kg, STZ, i.p.), Mg IV (5 mu g/kg, s.c.) and Dia+Ang W. The passive avoidance and Morris water maze (MWM) tests were used to evaluate learning and memory performance. Behavioral tests were carried out between 21 and 30 days after the initial Ang IV injection. Hippocampi were dissected and retained for biochemical and Western blot analysis. The Dia group exhibited the poorest behavioral results, while the Dia+Ang W group performed highest on the MWM task. Superoxide dismutase, glutathione peroxidase, and malondialdehyde levels increased significantly in the Dia group compared to Dia+Ang IV. Brain-derived neurotrophic factor (BDNF) and N-methyl-D-aspartate levels were significantly elevated, while levels of GABA(A) significantly decreased, in the Dia+Ang IV group compared to the Dia group. These findings suggest that peripheral administration of Ang IV ameliorated spatial memory in diabetic rats by decreasing hippocampal oxidative stress and BDNF levels.Publication Metadata only Impaired memory by hippocampal oxidative stress in rats exposed to 900 MHz electromagnetic fields is ameliorated by thymoquinone(2022-03-01T00:00:00Z) ÜSTÜNOVA, SAVAŞ; KILIÇ, AYSU; Bulut, Huri; Gurel-Gurevin, Ebru; ERİŞ, ALİ HİKMET; MERAL, İSMAİL; ÜSTÜNOVA, SAVAŞ; KILIÇ, AYSU; ERİŞ, ALİ HİKMET; MERAL, İSMAİLThe protective effects of thymoquinone on learning and memory abilities and on hippocampal oxidative stress in rats exposed to 900 MHz electromagnetic field radiation at s strength of 6.0 +/- 0.5 V/m for 1 h on each of 15 days was studied. Thymoquinone-treatment (10 mg/kg/day) of radiation-exposed rats resulted in better performance than that of radiation-exposed rats not receiving thymoquinone. Electromagnetic field-exposure led to increased hippocampal superoxide dismutase, glutathione peroxidase, and malondialdehyde levels and to lowered brain-derived neurotrophic factor level; thymoquinone treatment ameliorated all four parameters. Also, in the electromagnetic field-exposed group, N-methyl-D-aspartate receptor expression decreased, and gamma-aminobutyric-acid alpha receptor expression increased, which was reversed by thymoquinone treatment. In conclusion, treatment with the thymoquinone ameliorated the impact of the exposure to EMF and provided a hint on the mechanism involving oxidative stress.Publication Metadata only Effects of Thymoquinone On Erythrocyte Aggregation Kinetics: An In Vitro Study(2022-01-01T00:00:00Z) ÜSTÜNOVA, SAVAŞ; Uyuklu, Mehmet; MERAL, İSMAİL; ÜSTÜNOVA, SAVAŞ; MERAL, İSMAİL© 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.Erythrocyte aggregation is associated with a series of physio-pathological processes in body and according to this, current study was designed to examine how the thymoquinone (TQ) and a ggregation kinetics relation changes when the aggregation properties of erythrocyte suspensions are intervened using different approaches, such as changing the suspension environment or erythrocyte surface properties. Blood samples from 40 male Wistar albino rats (three months old weighing 250-300 g) were randomly divided into four groups, as 1) whole blood (control), 2) 1/2 dilution (plasma was diluted with PBS), 3) dextran 500 (1% dextran 500 was dissolved in plasma) and 4) glutaraldehyde. Blood samples were taken from abdominal aorta under anesthesia. The erythrocyte suspensions were incubated at 37°C for one hour in the absence or presence of TQ, with a final concentration of 1 mg/ml. Then, the electrical properties of erythrocyte suspensions were recorded, and the electrical capacitance was monitored using an inductance, capacitance, and resistance meter (LCR meter) at 100 kHz. It was found that TQ incubation decreased the aggregation index in control and dextran groups while increased it in 1/2 dilution and GA groups. It was also found that TQ incubation increased the time constant in whole blood and dextran while decreased it in 1/2 dilution and GA. The TQ can normalize the altered erythrocyte aggregation responses in both ways that is, it decreases the increased aggregation or increases the decreased aggregation. Therefore, it may have very critical role in rouleaux formation-induced circulatory disorders.Publication Open Access A low direct electrical signal attenuates oxidative stress and inflammation in septic rats(2021-09-01T00:00:00Z) Üstünova, Savaş; Hacıosmanoğlu, Ebru; Bulut, Huri; Kılıç, Aysu; Hekimoğlu, Emine Rümeysa; Tunc, Serkan; Kaygusuz, İrem; Tunc, Sevil; Tunc, Gulcin Beyza; Meral, Ismail; ÜSTÜNOVA, SAVAŞ; HACIOSMANOĞLU, EBRU; ELİBOL, BİRSEN; KILIÇ, AYSU; HEKİMOĞLU, EMİNE RÜMEYSA; MERAL, İSMAİLElectrical stimulation is proposed to exert an antimicrobial effect according to studies performed using bacterial and cell cultures. Therefore, we investigated the effects of electrification on inflammation in septic rats. Twenty-eight male Wistar albino rats were divided into 4 groups: healthy control (C), electrified healthy (E), sepsis (S), and electrified sepsis (SE) groups. Staphylococcus aureus (1 x 109 colonies) in 1 ml of medium was intraperitoneally injected into rats to produce a sepsis model. The rats in the E and SE groups were exposed to a low direct electrical signal (300 Hz and 2.5 volts) for 40 min and 1 and 6 h after bacterial infection. Immediately after the second electrical signal application, blood and tissue samples of the heart, lung, and liver were collected. An antibacterial effect of a low direct electrical signal was observed in the blood of rats. The effects of electrical signals on ameliorating changes in the histological structure of tissues, blood pH, gases, viscosity and cell count, activities of some important enzymes, oxidative stress parameters, inflammation and tissue apoptosis were observed in the SE group compared to the S group. Low direct electrical signal application exerts antibacterial, antioxidant, anti-inflammatory and antiapoptotic effects on septic rats due to the induction of electrolysis in body fluids without producing any tissue damage.Publication Metadata only Serum Electrolyte Values and Blood Oxidative Stress Parameters in Patients with Focal and Generalized Epilepsy(2023-02-01) Sarı G.; Kılıç A.; Uslu F.; Selek Ş.; Üstünova S.; Meral İ.; KILIÇ, AYSU; ÜSTÜNOVA, SAVAŞ; MERAL, İSMAİLPublication Metadata only Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats(2020-12-01T00:00:00Z) Üstünova, Savaş; Meral, İsmail; Koçyiğit, Abdürrahim; Kılıç, Aysu; Zeybek, Öykü; Meydan, Sedat; ÜSTÜNOVA, SAVAŞ; MERAL, İSMAİL; KOÇYİĞİT, ABDÜRRAHİM; KILIÇ, AYSU; MEYDAN, SEDATPublication Metadata only Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma(2022-03-01T00:00:00Z) Utkusavas, Ayfer; Gurevin, Ebru Gurel; YILMAZER, NADİM; ÜVEZ, AYÇA; Oztay, Fusun; Bulut, Huri; ÜSTÜNOVA, SAVAŞ; ESENER, OSMAN BEHZAT BURAK; SÖNMEZ, KIVILCIM; Kutucu, Deniz Erol; MERAL, İSMAİL; Dimas, Konstantinos; ARMUTAK, Elif İlkay; ÜSTÜNOVA, SAVAŞ; MERAL, İSMAİLCombined use of a chemotherapeutic agent and an autophagy inhibitor is a novel cancer treatment strategy. We investigated the effects of chloroquine (CQ) on lung pathology caused by both solid Ehrlich ascites carcinoma (EAC) and doxorubicin (DXR). A control group and eight experimental groups of adult female mice were inoculated subcutaneously with 2.5 x 10(6) EAC cells. DXR (1.5 mg/kg and 3 mg/kg) and CQ (25 mg/kg and 50 mg/kg) alone or in combination were injected intraperitoneally on days 2, 7 and 12 following inoculation with EAC cells. Lung tissue samples were examined using immunohistochemistry (IHC) for endothelial (eNOS), inducible nitric oxide synthase (iNOS) and neutrophil gelatinase-associated lipocalin (NGAL). Serum catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured using ELISA. We found decreased levels of iNOS and eNOS in the groups that received 1.5 mg/kg DXR alone and in combination with 25 mg/kg and 50 mg/kg CQ. Combined administration of DXR and CQ partially prevented disruption of alveolar structure. Levels of antioxidant enzymes and MDA were lower in all treated groups; the greatest reduction was observed in mice that received the combination of 25 mg/kg CQ + 1.5 mg/kg DXR. Levels of NGAL were elevated in all treated groups. We found that CQ ameliorated both EAC and DOX induced lung pathology in female mice with solid EAC by reducing oxidative stress.