Person: SÜMBÜL, BİLGE
13 results
Search Results
Now showing 1 - 10 of 13
Publication Open Access Discordance between Serum Neutralizing Antibody Titers and the Recovery from COVID-19(2020-09-25T00:00:00Z) Koç, Mm; Kalkan, Yazıcı; Çetin, Nesibe Selma; Doymaz, Mz; Sümbül, B; Durdu, B; YAZICI, MERVE; MERİÇ KOÇ, MELİHA; ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; OKAY, GÜLAY; DURDU, BÜLENT; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAThe recent pandemic of COVID-19 has caused a tremendous alarm around the world. Details of the infection process in the host have significant bearings on both recovery from the disease and on the correlates of the protection from the future exposures. One of these factors is the presence and titers of neutralizing Abs (NAbs) in infected people. In the current study, we set out to investigate NAbs in the recovered subjects discharged from the hospital in full health. Serum samples from a total of 49 documented consecutive COVID-19 subjects were included in the study. All the subjects were adults, and serum samples collected during the discharge were tested in viral neutralization, enzyme immunoassay (EIA), and Western immunoblot tests against viral Ags. Even though a majority of the recovered subjects had raised significant NAb titers, there is a substantial number of recovered patients (10 out of 49) with no or low titers of NAbs against the virus. In these cohorts as well as in patients with high NAb titers, viral Ag binding Abs were detectable in EIA tests. Both NAb titers and EIA detectable Abs are increased in patients experiencing a severe form of the disease, and in older patients the Ab titers were heightened. The main conclusion is that the recovery from SARS-CoV-2 infection is not solely dependent on high NAb titers in affected subjects, and this recovery process is probably produced by a complex interplay between many factors, including immune response, age of the subjects, and viral pathology.Publication Open Access Screening of mecC Gene in Methicillin Resistant Staphylococcus aureus Isolates(2022-04-01T00:00:00Z) Ceylan, Ayse Nur; SÜMBÜL, BİLGE; DOYMAZ, Mehmet Ziya; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAObjective: The diagnosis and treatment of mecC positive methicillin resistant Staphylococcus aureus (MRSA) isolates pose a significant problem in clinical microbiology and infectious disease practices. The studies on the frequency of mecC positive isolates in Turkey is rather scarce. In the present study, we aimed to investigate the presence of mecA, mecC, spa and pvu genes in MRSA strains isolated from various clinical specimens submitted to Clinical Microbiology Laboratories of Bezmialem Vakıf Hospital. Methods: We performed nucleic acid extraction and multiplex polymerase chain reaction (PCR) to 126 MRSA strains to detect mecC, mecA, spa and pvl genes. Results: According to the multiplex PCR results of 126 MRSA strains studied, 126 (100%) had mecA, 107 (85%) had spa, and 3 (2%) had pvl genes. We performed another polymerase chain reaction protocol and spa genes were identified in 19 of specimens, which were found negative priorly. Conclusion: Considering the factors that a university medical center where the study was conducted provided a tertiary healthcare service to a large metropolitan area in Istanbul and none of the isolates carried mecC gene might indicate that mecC gene carrying MRSA isolates did not pose a significant public health threat in Turkey.Publication Metadata only Antibiotic resistance patterns of Escherichia Coli strains isolated from urine cultures İdrar kültürlerinden izole edilen Escherichia coli suşlari’nin antibiyotik direnç paterni(2014-01-01) IRAZ, Meryem; Gültepe, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZİYA; SÜMBÜL, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZIYAPublication Metadata only Doğurganlık Çağındaki Kadınlarda Toksoplazma ve Rubella Seroprevalansı(2015-01-01) IRAZ, MERYEM; GÜLTEPE, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZİYA; SÜMBÜL, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZIYAPublication Metadata only HBsAg, Anti-HBs and Anti-HCV Seroprevalence of the Patients Applied to the Medical Faculty of Bezmialem Foundation University(2013-12-01T00:00:00Z) Iraz, Meryem; Gultepe, Bilge; DOYMAZ, Mehmet Ziya; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAObjective: The infections caused by hepatitis B and hepatitis C virus are still remain one of most important health problems for our country. The purpose of this study was to determine the frequency of HBsAg, anti-HBs and anti-HCV positivity in the patients who were admitted to the Research Hospital of the Medical Faculty of Bezmialem Vakif University between November 2011 and May 2013.Publication Metadata only Isolated from Perineal Abscess Actinomyces neuii subsp. neuii Case Report(2019-03-01) Akbaş, Emel; SÜMBÜL GÜLTEPE, BİLGE; Ersöz, Cevper; Ceylan, Ayşenur; Doymaz, Mehmet Ziya; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAPublication Metadata only A case of cerebral phaeohyphomycosis caused by Fonsecaea monophora, a neurotropic dematiaceous fungus, and a review of the literature(2015-03-01) Doymaz, Mehmet Z.; Gultepe, Bilge S.; Hakyemez, Ismail; CEVIK, Serdar; ASLAN, Turan; DOYMAZ, MEHMET ZIYA; SEYİTHANOĞLU, MEHMET HAKAN; HAKYEMEZ, İSMAİL NECATİ; SÜMBÜL, BİLGEThe Fonsecaea species, which are the leading causes of chromoblastomycosis, are not considered neurotropic fungal agents. Fonsecaea pedrosoi is the primary species in the genus and is usually isolated from chromoblastomycosis cases. However, the recently distinguished species F. monophora has been reported in a few cerebral phaeohyphomycosis cases. Here, a case of cerebral phaeohyphomycosis caused by Fonsecaea monophora is presented in a 71-year-old female subject with chronic diabetes mellitus and hypertension. The identification of F. monophora was made through mycological and molecular analysis, and an isolate was differentiated from the closely related F.pedrosoi by sequence data on key bases on the ribosomal internal transcribed spacer region. The case was successfully treated with surgical and medical approaches, and the patient has remained healthy and stable after a ten-month follow up. Given the increasing incidence of this type of infection of the central nervous system (CNS), this case provides further support for the consideration that F. monophora might represent a neurotropic agent.Publication Open Access Hand, Foot, and Mouth Disease Caused by Coxsackievirus A6: A Preliminary Report from Istanbul.(2019-01-01) Ceylan, AN; Turkmen, AV; Turel, O; Gultepe, BİLGE; Inan, E; Doymaz, MEHMET ZİYA; TÜREL, ÖZDEN; SÜMBÜL, BİLGE; VEHAPOĞLU TÜRKMEN, AYSEL; DOYMAZ, MEHMET ZIYAHand, foot, and mouth disease (HFMD) is caused by various serotypes of Enterovirus genus. Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) were known to be the only responsible agents for these epidemics; however, this opinion was challenged after the detection that coxsackievirus A6 (CV-A6) was the responsible species for the outbreak in Finland in 2008. HFMD is frequently seen in Turkey, and no detailed study on its clinical and microbiological epidemiology has previously been reported. The present study addresses this question. Twenty-seven patient samples collected between 2015 and 2017 were included in the study. Typing was conducted by RT-PCR and the sequencing applied directly to patient's samples and as well as to the viral cultures with pan-enterovirus and serotype-specific primers. The presence of Enterovirus in 12 of 27 HFMD samples was shown with RT-PCR. The causative agent for three of these 12 samples was CV-A16, one of the most frequent two serotypes around the world, and the remaining nine samples was CV-A6. The findings of the study are relevant since it pertains to the molecular epidemiology of HFMD in Turkey, a gateway country where different serotypes might be circulating and transmitted. The findings also support the notion that CV-A6 cases are rising in number, which has caused more severe clinical features and widespread rashes in recent outbreaks. Hand, foot, and mouth disease (HFMD) is caused by various serotypes of Enterovirus genus. Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) were known to be the only responsible agents for these epidemics; however, this opinion was challenged after the detection that coxsackievirus A6 (CV-A6) was the responsible species for the outbreak in Finland in 2008. HFMD is frequently seen in Turkey, and no detailed study on its clinical and microbiological epidemiology has previously been reported. The present study addresses this question. Twenty-seven patient samples collected between 2015 and 2017 were included in the study. Typing was conducted by RT-PCR and the sequencing applied directly to patient’s samples and as well as to the viral cultures with pan-enterovirus and serotype-specific primers. The presence of Enterovirus in 12 of 27 HFMD samples was shown with RT-PCR. The causative agent for three of these 12 samples was CV-A16, one of the most frequent two serotypes around the world, and the remaining nine samples was CV-A6. The findings of the study are relevant since it pertains to the molecular epidemiology of HFMD in Turkey, a gateway country where different serotypes might be circulating and transmitted. The findings also support the notion that CV-A6 cases are rising in number, which has caused more severe clinical features and widespread rashes in recent outbreaks.Publication Metadata only Erişkin Yaş Gruplarında Hepatit A Seroprevalansı(2015-01-01) IRAZ, MERYEM; GÜLTEPE, BİLGE; DOYMAZ, MEHMET ZİYA; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAPublication Metadata only Antibiotic resistance patterns of Escherichia Coli strains isolated from urine cultures(2014-01-01) IRAZ, MERYEM; GÜLTEPE, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZİYA; SÜMBÜL, BİLGE; CEYLAN, AYŞE NUR; DOYMAZ, MEHMET ZIYA