Person: EROL, EBRU
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Publication Metadata only Di-, and Triterpenoids Isolation and LC-MS Analysis of Salvia marashica Extracts with Bioactivity Studies(2021-11-01T00:00:00Z) Toraman, Gulbahar O. Alim; Aydin, Sibel Kiran; Ertas, Abdulselam; Boga, Mehmet; EROL, EBRU; Saygi, Tuba Kusman; Halfon, Belkis; TOPÇU, GÜLAÇTI; EROL, EBRU; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTIIn this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salvia marashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of these extracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidant activity was determined by four complementary methods including inhibition of lipid peroxidation (by beta-carotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity and CUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity tests were carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. The triterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone and methanol extracts isolated by chromatographic methods. Their structures were determined based on spectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone or methanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate, lup-12, 20(29)-diene, lup-20(29)-ene, alpha-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroid beta-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene (2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in the present study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MS analysis.Publication Metadata only Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii - A forgotten endemic angiosperm of Turkey(2016-09-01T00:00:00Z) Yildiztekin, Fatma; Nadeem, Said; Erol, EBRU; Yildiztekin, Mahmut; Tuna, Atilla L.; Ozturk, Mehmet; EROL, EBRUContext We report the first ever chemical/biochemical study on Crocus mathewii Kerndorff (Iridaceae) - a Turkish endemic angiosperm. This plant has never been explored for its phytochemistry and bioactivities.Objective This study explores C. mathewii corm and aerial parts for the chemical and biological properties of hexane, ethyl acetate, methanol and water fractions of the extracts.Material and methods Plant material (20g) was extracted by methanol (250mLx5, 3 days each) and fractioned into hexane, ethyl acetate, methanol and water. All fractions were subjected to -carotene-linoleic acid, DPPH, ABTS(+), CUPRAC, metal chelating and tyrosinase inhibition activities. Hexane fractions were submitted to GC-MS analysis.Results Ethyl acetate fractions showed excellent IC50 values in DPPH (aerial 36.210.76 and corm 33.87 +/- 0.02mg/L) and ABTS(+) (aerial 33.01 +/- 0.79 and bulb 27.87 +/- 0.33mg/L); higher than the IC50 of the standard -tocopherol (DPPH 116.25 +/- 1.97; ABTS 52.64 +/- 0.37mg/L), higher than BHA in DPPH (57.31 +/- 0.25mg/L), but slightly lower in ABTS (19.86 +/- 2.73mg/L). Methanol extract of aerial parts also showed higher activity than -tocopherol in DPPH (85.56 +/- 11.51mg/L) but slightly less (72.90 +/- 3.66mg/L) than both the standards in ABTS. Linoleic (aerial 53.9%, corm 43.9%) and palmitic (aerial 22.2%, corm 18%) were found as the major fatty acids.Discussion and conclusion Some fractions of C. mathewii showed higher antioxidant activities than the standards. There is a need to explore more about this plant.Publication Metadata only Berberis Plants-Drifting from Farm to Food Applications, Phytotherapy, and Phytopharmacology(2019-10-01T00:00:00Z) Salehi, Bahare; Selamoglu, Zeliha; Sener, Bilge; Kilic, Mehtap; Jugran, Arun Kumar; de Tommasi, Nunziatina; Sinisgalli, Chiara; Milella, Luigi; Rajkovic, Jovana; Morais-Braga, Maria Flaviana B.; Bezerra, Camila F.; Rocha, Janaina E.; Coutinho, Henrique D. M.; Ademiluyi, Adedayo Oluwaseun; Shinwari, Zabta Khan; Jan, Sohail Ahmad; Erol, EBRU; Ali, Zulfiqar; Ostrander, Elise Adrian; Sharifi-Rad, Javad; de la Luz Cadiz-Gurrea, Maria; Taheri, Yasaman; Martorell, Miguel; Segura-Carretero, Antonio; Cho, William C.; EROL, EBRUThe genus Berberis includes about 500 different species and commonly grown in Europe, the United States, South Asia, and some northern areas of Iran and Pakistan. Leaves and fruits can be prepared as food flavorings, juices, and teas. Phytochemical analysis of these species has reported alkaloids, tannins, phenolic compounds and oleanolic acid, among others. Moreover, p-cymene, limonene and ocimene as major compounds in essential oils were found by gas chromatography. Berberis is an important group of the plants having enormous potential in the food and pharmaceutical industry, since they possess several properties, including antioxidant, antimicrobial, anticancer activities. Here we would like to review the biological properties of the phytoconstituents of this genus. We emphasize the cultivation control in order to obtain the main bioactive compounds, the antioxidant and antimicrobial properties in order to apply them for food preservation and for treating several diseases, such as cancer, diabetes or Alzheimer. However, further study is needed to confirm the biological efficacy as well as, the toxicity.Publication Metadata only Inhibition of iNOS Induction and NF-kappa B Activation by Taste Compounds from the Edible Mushroom Tricholoma caligatum (Viv.) Ricken(2020-01-01T00:00:00Z) Erol, EBRU; Ali, Zulfiqar; Ozturk, Mehmet; Khan, Shabana; Khan, Ikhlas A.; EROL, EBRUTricholoma caligatum (Viv.) Ricken is an edible mushroom that belongs to matsutake group. The first chemical investigation of the three different extracts of Tricholoma caligatum resulted in two new compounds, 8-demethoxylascivol (1) and 8-epi-lascivol (2) and six known compounds, lascivol (3), trametenolic acid (4), ergosterol (5), ergosterol peroxide (6), 5 alpha, 6 alpha-epoxyergosterol (7), and cerebroside B (8). Their structures were elucidated by spectroscopic analyses including 1D and 2D NMR data. The biological activities of all the compounds were evaluated toward multiple targets related to inflammation and metabolic disorder such as NF-kappa B, iNOS and ROS. The findings of this study reveal that the edible mushroom Tricholoma caligatum could be a potential source for anti-inflammatory bioactive metabolites.Publication Open Access Anti-SARS-CoV-2 and cytotoxic activity of two marine alkaloids from green alga Caulerpa cylindracea Sonder in the Dardanelles(2022-10-01T00:00:00Z) Erol, Ebru; Alim Toraman, Gulbahar Ozge; Orhan, Muge Didem; Avsar, Timucin; Akdemir, Atilla; Okudan, Emine Sukran; Topcu, Gulacti; EROL, EBRU; AKDEMİR, ATİLLA; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTICaulerpa cylindracea Sonder is a green alga belonging to the CauIerpaceae family. This is the first chemical investigation of C. cylindracea in the Dardanelles which resulted in the isolation of four compounds, caulerpin (1), monomethyl caulerpinate (2), beta-sitosterol (3), and palmitic acid (4). Their structures were elucidated by spectroscopic analyses including 1D- and 2D NMR and mass. The isolated compounds 1 and 2 were tested against the SARS-CoV-2 viral targets spike protein and main protease (3CL) enzyme, and both compounds significantly inhibit the interaction of spike protein and ACE2, while the main protease activity was not significantly reduced. Docking studies suggested that compounds 1 and 2 may bind to the ACE2 binding pocket on spike, and compound 2 may aka bind to an allosteric site on spike. As such, these compounds may inhibit the spike-ACE2 complex formation competitively and/or allosterically and have the potential to be used against SARS-CoV-2 virus infection. In addition, compounds 1 and 2 showed at [east two-fold higher cytotoxicity against breast cancer cell Lines MCF7 and MDA-MB-231 compared to the CCD fibroblast control cell LinePublication Metadata only Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica(2017-01-01T00:00:00Z) Ferhat, Maria; Erol, EBRU; Beladjila, Khadidja Aya; Cetintas, Yunus; Duru, Mehmet Emin; Ozturk, Mehmet; Kabouche, Ahmed; Kabouche, Zahia; EROL, EBRUContext: Stachys guyoniana Noe ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine.