Person: TOPÇU, GÜLAÇTI
Search Results
Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines
2020-01-01T00:00:00Z, GAZİOĞLU, IŞIL, Semen, Sevcan, Acar, Ozden Ozgun, KOLAK, Ufuk, Sen, Alaattin, TOPÇU, GÜLAÇTI, GAZİOĞLU, IŞIL, TOPÇU, GÜLAÇTI
Context: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.
Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption
2019-01-01T00:00:00Z, Sen, Alaattin, Ayar, Buket, Yilmaz, Anil, Acar, Ozden Ozgun, Turgut, Gurbet Celik, TOPÇU, GÜLAÇTI, YILMAZ, ANIL, TOPÇU, GÜLAÇTI
Teucrium species have been used in folk medicine as antidiabetic, antiinflammatory, antiulcer, and antibacterial agents. We have explored in vitro antidiabetic impacts of 2 natural diterpenoids, alysine A and alysine B, isolated from Teucrium alyssifolium. The lactate dehydrogenase (LDH) cytotoxicity assay, glucose uptake test, glucose utilization (glycogen content) test, glucose transport test, glucose absorption (a-glucosidase activity) test, insulin secretion test, RNA isolation and cDNA synthesis assay, qPCR quantification assays, and statistical analyses were carried out in the present study. Alysine A exerted the following effects at non-cytotoxic doses:
Investigation of Anticholinesterase Activity of a Series of Salvia Extractsand the Constituents of Salvia staminea
2013-03-01, Topçu, Gülaçtı, Boğa, Mehmet, Kolak, Ufuk, Öztürk, Mehmet, Dirmenci, Tuncay, Bahadori, Fatemeh, Bütün, Burcu, TOPÇU, GÜLAÇTI, BAHADORİ, FATEMEH, BÜTÜN, BURCU
Preclinical studies evaluating the therapeutic efficacy of novel natural compound olean-12-en-28-ol, 3β pentacosanoate for multiple sclerosis
2022-09-26, Şenol H., Özgün-Acar Ö., Dağ A., Eken A., Acar B., Aktaş Pepe N., Güner H., Aykut Z. G., Çevik Kaplan S., Ayaz Güner Ş., et al., ŞENOL, HALIL, DAĞ, AYDAN, TOPÇU, GÜLAÇTI
Effect of a combination in situ gel-forming nasal delivery system on nasal mucosa damage repair in rabbits
2017-10-17, AKSOY, FADLULLAH, DOĞAN, REMZİ, OZTURAN, ORHAN, ALTUNTAŞ, EBRU, YENER, FATMA GÜLGÜN, TOPÇU, GÜLAÇTI, GÜLER, BERİL, AKSOY, FADLULLAH, DOĞAN, REMZI, ÖZTURAN, ORHAN, TOPÇU, GÜLAÇTI, GÜLER, BERIL
Isolation and Structure Elucidation of the Constituents of Some Anatolian Nepeta Species with Bioactivities
2013-09-02T00:00:00Z, Topçu, Gülaçtı, Yılmaz, Anıl, TOPÇU, GÜLAÇTI
Synthesis of New Oleanane Triterpenoids Including Fatty Acids Esters and Investigation of Their in vitro Cytotoxic Effects on 3T3 Fibroblast and PC3 Prostate Cancer Cell Lines
2019-12-19, Dağ, Aydan, Topçu, Gülaçtı, Atasoy, Sezen, Aktaş, Asude Sena, Şenol, Halil, ŞENOL, HALIL, DAĞ, AYDAN, ATASOY, SEZEN, TOPÇU, GÜLAÇTI
Biological Activities and Chemical Composition of Ferulago idaea
2015-06-12, Aslan, Esin, DİNCEL, DEMET, Çakar, Begüm, TOPÇU, GÜLAÇTI, DİNCEL, DEMET, TOPÇU, GÜLAÇTI
Evaluation of fruit extracts of six Turkish Juniperus species for their antioxidant, anticholinesterase and antimicrobial activities
2011-03-30T00:00:00Z, Ozturk, Mehmet, Tumen, Ibrahim, Ugur, Aysel, Aydogmus-Ozturk, Fatma, Topcu, GÜLAÇTI, TOPÇU, GÜLAÇTI
BACKGROUND: Juniperus L. (Cupressaceae) species are mostly spread out in the Northern Hemisphere of the world, and some of them are used as folkloric medicines. The fruits of some species are eaten. Since oxidative stress is one of the reasons for neurodegeneration and is associated with the Alzheimer-s disease (AD), the extracts prepared from the fruits of six Juniperus species were screened for their antioxidant activity. Therefore, the extracts were also evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), which are chief enzymes in the pathogenesis of AD. In addition, antimicrobial activity was also evaluated.
Ursolik Asit ve Oleanolik Asitten Hareketle alfa-Amirin ve beta-Amirin Sentezi için Yeni ve Etkin Bir Yöntem
2017-04-29, SEÇEN, HASAN, ŞENOL, HALİL, TOPÇU, GÜLAÇTI, TUNCAY, SALİH, ÖCAL, NÜKET, ŞENOL, HALIL, TOPÇU, GÜLAÇTI