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TOPÇU, GÜLAÇTI

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Author: Kolak, Ufuk × Author: Topcu, GÜLAÇTI ×

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    Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation
    (2011-05-01T00:00:00Z) Ozturk, Mehmet; Kolak, Ufuk; Topcu, GÜLAÇTI; Oksuz, Sevil; CHOUDHARY, M. Iqbal; TOPÇU, GÜLAÇTI
    From the acetone extract of Micromeria cilicica, two new (1 and 2) and five known (3-7) compounds were obtained through radical-scavenging activity guided isolation. Structures of the compounds were identified as piperitone 7-O-beta-D-glucoside (1), isothymonin 4--methyl ether (2), sudachitin (3), isomucronulatol (4), rutin (5), ursolic acid (6) and saccharose (7), based on UV, 1D-and 2D-NMR and mass spectroscopic techniques. The antioxidant potentials of the extract and the isolated compounds were established by using three radical-scavenging assays, namely, DPPH. scavenging, O-2(center dot-) scavenging and ABTS(center dot+) scavenging, besides beta-carotene bleaching assay. Particularly, the acetone extract showed a strong inhibition of lipid peroxidation by beta-carotene bleaching assay, with a result close to that of (+)-catechin. Among the pure compounds, rutin (5) showed the strongest lipid peroxidation inhibition and antiradical activity while both sudachitin (3) and isomucronulatol (4) exhibited noticeable ABTS(center dot+) scavenging activity. The anticholinesterase activity of the compounds (1-7) was also determined. Against acetylcholinesterase, they exhibited weak inhibition while compounds 3, 4 and 6 exhibited moderate inhibition against butyrylcholinesterase. (C) 2010 Elsevier Ltd. All rights reserved.
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    Antioxidant, anticholinesterase, and antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm.
    (2014-01-01) Ertas, Abdulselam; Boga, Mehmet; Hasimi, Nesrin; Yesil, Yeter; GOREN, AHMET CEYHAN; Topcu, GÜLAÇTI; Kolak, Ufuk; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI
    This study was the first fatty acid analysis and biological activity report on an endemic Achillea species, Achillea cappadocica Hausskn. et Bornm. The major compounds in the fatty acid were identified as oleic (34.7%), palmitic (23.1%), and linoleic acids (20.6%). The methanol and water extracts indicated higher ABTS cation radical scavenging activity than that of alpha-tocopherol and BHT at 100 mu g/mL. The acetone extract showed 70.62% inhibitory activity against butyrylcholinesterase at 200 mu g/mL. The acetone and methanol extracts exhibited moderate antimicrobial activity.
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    Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice
    (2016-09-15) Ozgun-Acar, Ozden; Celik-Turgut, Gurbet; Gazioglu, IŞIL; Kolak, Ufuk; Ozbal, Seda; Ergur, Bekir U.; Arslan, Sevki; Sen, Alaattin; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI
    Since ancient times, Capparis species have been widely used in traditional medicine to treat various diseases. Our recent investigations have suggested Capparis ovata-s potential anti-neuroinflammatory application for the treatment of multiple sclerosis (MS). The present study was designed to precisely determine the underlying mechanism of its anti-neuroinflammatory effect in a mouse model of MS. C. ovata water extract (COWE) was prepared using the plant-s fruit, buds, and flower parts (Turkish Patent Institute, PT 2012/04,093). We immunized female C57BL/6 J mice with MOG(35_55)/CFA. COWE was administered at a daily dose of 500 mg/Kg by oral gavage either from the day of immunization (T1) or at disease onset (12) for 21 days. Gene expression analysis was performed using a Mouse Multiple Sclerosis RT2 Profiler PCR Array, and further determinations and validations of the identified genes were performed using qPCR. Whole-genome transcriptome profiling was analyzed using Agilent SurePrint G3 Mouse GE 8X60K microarrays. Immunohistochemical staining was applied to brain sections of the control and treated mice to examine the degree of degeneration. COWE was further fractionated and analyzed phytochemically using the Zivak Tandem Gold Triple Quadrupole LC/MS-MS system. COWE remarkably suppressed the development of EAE in T1, and the disease activity was completely inhibited. In the T2 group, the maximal score was significantly reduced compared with that of the parallel EAE group. The COWE suppression of EAE was associated with a significantly decreased expression of genes that are important in inflammatory signaling, such as TNF alpha, IL6, NF-kappa B, CCL5, CXCL9, and CXCK10. On the other hand, the expression of genes involved in myelination/remyelination was significantly increased. Immunohistochemical analysis further supported these effects, showing that the number of infiltrating immune cells was decreased in the brains of COWE-treated animals. In addition, differential expression profiling of the transcriptome revealed that COWE treatment caused the down regulation of a group of genes involved in the immune response, inflammatory response, antigen processing and presentation, B-cell-mediated immunity and innate immune response. Collectively, these results suggest anti-neuroinflammatory mechanisms by which COWE treatment delayed and suppressed the development of EAE and ameliorated the disease in mice with persistent clinical signs. (C) 2016 Elsevier B.V. All rights reserved.
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    Stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata having anti-inflammatory and immunomodulatory actions could be of interest for use in multiple sclerosis
    (2017-08-30) Sen, Alaattin; Acar, Ozden Ozgun; Gazioglu, IŞIL; Kolak, Ufuk; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI
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    Anti-neuroinflammatory effect of butanolic subextract of Capparis ovata water extract used as an alternative and complementary treatment for multiple sclerosis
    (2014-10-15) Sen, Alaattin; Topcu, GÜLAÇTI; Ozgun, Ozden; Kolak, Ufuk; Hacibekiroglu, Isil; Celik, Gurbet; Arslan, Sevki; TOPÇU, GÜLAÇTI
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    Two new indole alkaloids from Vinca herbacea L.
    (2011-12-01T00:00:00Z) Boga, Mehmet; Kolak, Ufuk; Topcu, GÜLAÇTI; Bahadori, Fatemeh; Kartal, Murat; FARNSWORTH, Norman R.; TOPÇU, GÜLAÇTI; BAHADORİ, FATEMEH; KARTAL, MURAT
    Six indole alkaloids were isolated from the aerial parts of Vinca herbacea Waldst. et Kit. Structures of the isolated compounds were elucidated as two new alkaloids, 10-methoxyraucaffrinoline (1) and 11-methoxyburnamine (2), and four known alkaloids, picrinine (3), burnamine (4), caboxine A (5) and elegantine (6) by intensive 1D-and 2D-NMR spectroscopic techniques. Antioxidant and anticholinesterase activities were investigated for the methanol, alkaloidal and non-alkaloidal extracts of V. herbacea and isolated alkaloids (1-3, 6). The alkaloidal extract was found to be active in the three tested antioxidant assays and exhibited significant lipid peroxidation inhibitory and DPPH free radical scavenging activities. The alkaloidal extract also exhibited high anticholinesterase activity against AChE and BChE enzymes similar to galanthamine. However, none of the tested alkaloids 1-3, and 6 were found to be active in the tested antioxidant and anticholinesterase systems. The alkaloids 1, 2, 4, and 6 were also evaluated for cytotoxic activity against the MCF7 and HT29 cell lines, and showed no activity. (C) 2011 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.
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    PublicationOpen Access
    Terpenoids, essential oil composition and fatty acids profile, and biological activities of Anatolian Salvia fruticosa Mill.
    (2013-01-01) Topcu, GÜLAÇTI; Ozturk, Mehmet; Kusman, TUBA; BARLA DEMIRKOZ, Ayse Asli; Kolak, Ufuk; Ulubelen, Ayhan; TOPÇU, GÜLAÇTI; KUŞMAN, TUBA
    The hexane and dichloromethane extracts, obtained by re-extraction of the methanol extract of Salvia fruticosa Mill., afforded 7 diterpenoids (carnosol, carnosic acid, carnosic acid 12-methyl ether, rosmadial, isorosmanol, ferruginol, and manool), 4 triterpenoids (alpha-amyryltetracosanoate, oleanolic acid, ursolic acid, and erythrodiol), a steroid (3-acetylsitosterol), and a flavone (salvigenin). The galls (apples) of the collected plant were separately extracted with hexane to afford fatty acids composed mainly of oleic acid (29%), palmitoleic acid (29%), and stearic acid (23.20%), which exhibited high anticholinesterase activity, particularly against BChE. The essential oil, obtained from the aerial parts of the plant, exhibited high AChE inhibitory activity, consisting of mainly 1,8-cineol (58.89%). The antioxidant activity according to 6 complementary tests and anticholinesterase potential of the methanol extracts and triterpenoids alpha-amyryltetracosanoate, oleanolic acid, ursolic acid, and sitosterol acetate were also investigated, and methanol extract exhibited the highest antioxidant and anticholinesterase activity, surpassing the other tested extracts and pure compounds.
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    Characterization of the Chemical Profile of Euphorbia Species from Turkey by Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), and Liquid Chromatography-Ion Trap-Time-of-Flight-Mass Spectrometry (LC-IT-TOF-MS) and Chemometric Analysis
    (2019-05-03) Yener, Ismail; Ertas, Abdulselam; Yılmaz, Mustafa Abdullah; Olmez, Ozge Tokul; Yilmaz, Pelin Koseoglu; Yesil, Yeter; Kaplaner, Erhan; Ozturk, Mehmet; Temel, Hamdi; Kolak, Ufuk; Topcu, GÜLAÇTI; TOPÇU, GÜLAÇTI
    The Euphorbiaceae family comprises of about 300 genera and 5000 species primarily distributed in America and tropical Africa. The Euphorbia genus is represented by 105 species and locally named as -Sutlegen- and -Xasil- in Turkey. The present study aimed to determine the chemical constituents of E. aleppica, E. eriophora, E. macroclada, E. grisophylla, E. seguieriana subsp. seguieriana, E. craspedia, E. denticulata, E. falcata, and E. fistulosa, and classify them by utilizing the chemometric techniques of principal component analysis (PCA) and hierarchical cluster analysis (HCA). Linoleic acid, 17-tetratriacontane, palmitic acid, and hexatriacontane were the major fatty acids from the gas chromatography-mass spectrometry (GC/MS) analyses. Characterization of 268 constituents of the studied species was achieved by liquid chromatography-ion trap-time-of-flight-mass spectrometry (LC-IT-TOF-MS). Furthermore, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous quantitative determination of 11 compounds (quinic acid, protocatechuic acid, rutin, hesperidin, eugenol, p-coumaric acid, piceatannol, scopoletin, dl-kavain, chrysophanic acid, and resiniferatoxin) in these species. The developed method was validated for the linearity, limit of detection, limit of quantification, repeatability, and recovery.
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    PublicationOpen Access
    Chemical profile and biological activities of Veronica thymoides subsp pseudocinerea
    (2015-03-01) Ertas, Abdulselam; Boga, Mehmet; Kizil, Murat; Ceken, Bircan; GOREN, AHMET CEYHAN; Hasimi, Nesrin; Demirci, Serpil; Topcu, GÜLAÇTI; Kolak, Ufuk; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI
    Context: In Turkey, Veronica species (Plantaginaceae) have been used as a diuretic and for wound healing in traditional medicine. Objective: To examine the fatty acid and essential oil profiles, the antioxidant, anticholinesterase, antimicrobial, and DNA damage effects of Veronica thymoides P.H. Davis subsp. pseudocinerea M.A. Fischer as a potential source of natural active compounds. Materials and methods: GC/MS was used to analyze essential oil and fatty acid obtained from whole plant. The antioxidant activity was evaluated by the beta-carotene-linoleic acid test system, DPPH-free and ABTS cation radicals scavenging, and cupric reducing antioxidant capacity assays. The anticholinesterase and antimicrobial activities were determined by Ellman and broth macrodillution methods, respectively. The effect of the methanol extract on DNA cleavage was investigated. Results: Hexatriacontene (21.0%) was found to be the main constituent in essential oil, and linoleic acid (25.2%) and palmitic acid (20.6%) in fatty acid. Methanol extract demonstrated the best IC50 values in lipid peroxidation (49.81 +/- 0.31 mu g/ml) and DPPH-free radical scavenging activity (15.32 +/- 0.17 mu g/ml). Methanol and water extracts possessed strong ABTS cation radical scavenging activity with IC50 values 9.15 +/- 0.28 and 8.90 +/- 0.14 mu g/ml, respectively. The acetone extract exhibited moderate butyrylcholinesterase inhibitory activity. The highest antimicrobial activity was determined in methanol extract against Escherichia coli with 31.25 mu g/ml MIC value. Inhibition of methanol extract on plasmid DNA cleavage by OH radicals was found to be 93.32% at 500 mu g/ml. Conclusion: The methanol extract having strong antioxidant and DNA damage effects could be investigated phytochemically to find natural active compounds.