Person: GAZİOĞLU, IŞIL
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Publication Open Access Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines(2020-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Semen, Sevcan; Acar, Ozden Ozgun; KOLAK, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIContext: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.Publication Metadata only Anti-inflammatory Effects of UA and OPC Isolated from Capparis ovata(2019-09-27T00:00:00Z) KALE, ELİF; ÖZGÜN ACAR, ÖZDEN; Abilo Alfa, Hajarat; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI; ŞEN, ALAATTİN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only Capparis ovate ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice(2015-09-01) Ozgun, O.; Turgut, G. C.; Gazioglu, IŞIL; Kolat, U.; Ozbal, S.; Ergur, B. U.; Arslan, S.; Topcu, GÜLAÇTI; Sen, A.; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only Anticarcinogenic effect of a novel alkaloid: 1H-indole-2-hydroxy, 3-carboxylic acid(2019-09-27T00:00:00Z) ÖZGÜN ACAR, ÖZDEN; KALE, ELİF; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI; ŞEN, ALAATTİN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only Analysis of 1H-indole-2-hydroxy, 3 carboxylic acid isolated from Capparis ovata for the treatment of MS: lack of evidence for the efficacy(2017-09-08) ÖZGÜN ACAR, ÖZDEN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI; ŞEN, ALAATTİN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only Beneficial actions of dichloromethane sub-fraction of the COWE extract for multiple sclerosis: a potential therapeutic role(2016-09-01) Acar, O. Ozgun; Sen, A.; Topcu, G.; Kolak, U.; Arslan, S.; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞILPublication Metadata only Investigation of the Anti-inflammatory Potential of Glucocapparin Isolated from Capparis ovata(2017-07-05) ÖZGÜN ACAR, ÖZDEN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI; ŞEN, ALAATTİN; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only A potential therapeutic role in multiple sclerosis for stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata(2017-07-01) Acar, Ozden Ozgun; GAZİOĞLU, IŞIL; Kolak, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIMultiple sclerosis (MS) is an autoimmune disease of the human central nervous system. It is one of the most common neurological disorders around the world and there is still no complete cure for MS. Purification of a terpenoid from Capparis ovata was carried out and its structure was elucidated as stigmast-5,22-dien-3 beta-ol, myristate (3 beta, 22E-stigmasteryl myristate; SDM) by NMR and mass spectral analyses. No information regarding its any health effect is available in the literature. In the present study, we have described its effects on inflammatory factors such as the expression levels of cytokines, chemokines and adhesion molecules as well as apoptosis/infiltration and myelination in SH-SY5Y cells. The expression levels of proinflammatory or inflammatory cytokines and chemokines such as NF-.B1, CCL5, CXCL9, CXCL10 and HIF1A along with T-cell activating cytokines such as IL-6 and TGFB1 were significantly downregulated with SDM treatment. Moreover, the expression levels of the main myelin proteins such as MBP, MAG and PLP that are essential for healthy myelin architecture were significantly up-regulated. The results presented in this study strongly suggest that the SDM offers a unique possibility to be used with autoimmune diseases, including MS due to its activity on the manipulation of cytokines and the promotion of myelin formation.Publication Metadata only Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice(2016-09-15) Ozgun-Acar, Ozden; Celik-Turgut, Gurbet; Gazioglu, IŞIL; Kolak, Ufuk; Ozbal, Seda; Ergur, Bekir U.; Arslan, Sevki; Sen, Alaattin; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTISince ancient times, Capparis species have been widely used in traditional medicine to treat various diseases. Our recent investigations have suggested Capparis ovata-s potential anti-neuroinflammatory application for the treatment of multiple sclerosis (MS). The present study was designed to precisely determine the underlying mechanism of its anti-neuroinflammatory effect in a mouse model of MS. C. ovata water extract (COWE) was prepared using the plant-s fruit, buds, and flower parts (Turkish Patent Institute, PT 2012/04,093). We immunized female C57BL/6 J mice with MOG(35_55)/CFA. COWE was administered at a daily dose of 500 mg/Kg by oral gavage either from the day of immunization (T1) or at disease onset (12) for 21 days. Gene expression analysis was performed using a Mouse Multiple Sclerosis RT2 Profiler PCR Array, and further determinations and validations of the identified genes were performed using qPCR. Whole-genome transcriptome profiling was analyzed using Agilent SurePrint G3 Mouse GE 8X60K microarrays. Immunohistochemical staining was applied to brain sections of the control and treated mice to examine the degree of degeneration. COWE was further fractionated and analyzed phytochemically using the Zivak Tandem Gold Triple Quadrupole LC/MS-MS system. COWE remarkably suppressed the development of EAE in T1, and the disease activity was completely inhibited. In the T2 group, the maximal score was significantly reduced compared with that of the parallel EAE group. The COWE suppression of EAE was associated with a significantly decreased expression of genes that are important in inflammatory signaling, such as TNF alpha, IL6, NF-kappa B, CCL5, CXCL9, and CXCK10. On the other hand, the expression of genes involved in myelination/remyelination was significantly increased. Immunohistochemical analysis further supported these effects, showing that the number of infiltrating immune cells was decreased in the brains of COWE-treated animals. In addition, differential expression profiling of the transcriptome revealed that COWE treatment caused the down regulation of a group of genes involved in the immune response, inflammatory response, antigen processing and presentation, B-cell-mediated immunity and innate immune response. Collectively, these results suggest anti-neuroinflammatory mechanisms by which COWE treatment delayed and suppressed the development of EAE and ameliorated the disease in mice with persistent clinical signs. (C) 2016 Elsevier B.V. All rights reserved.Publication Metadata only Stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata having anti-inflammatory and immunomodulatory actions could be of interest for use in multiple sclerosis(2017-08-30) Sen, Alaattin; Acar, Ozden Ozgun; Gazioglu, IŞIL; Kolak, Ufuk; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI