Person: GAZİOĞLU, IŞIL
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Publication Metadata only Effect of roasting on antioxidant and anticholinesterase capacities of coffee(2014-01-01) Yilmaz, Pelin Koseoglu; Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILThe aim of the study was to investigate the effect of the roasting degree on total phenolic and flavonoid contents, antioxidant and anticholinesterase activities of the hexane, acetone, ethanol and water extracts of coffee. The antioxidant capacity of the water extracts prepared according to the brewing procedure of Turkish coffee was measured for the first time by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and cupric ion reducing antioxidant capacity (CUPRAC) assays. The in vitro anticholinesterase activity of the coffee extracts and caffeine was determined for the first time. Water extracts of the coffee samples exhibited the highest 2,2--azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging, DPPH free radical scavenging and CUPRAC effects. The hexane extract of coffee roasted for 25 min showed the highest acetylcholinesterase inhibitory activity among the tested extracts. Caffeine, which is known to have therapeutic effect against Alzheimer-s disease, was found to possess almost the same anticholinesterase activity as galanthamine. The roasting degree had an irregular effect on the antioxidant and anticholinesterase activities of the coffee extracts. The caffeine contents in extracts of coffee roasted for 25 min, which had strong anticholinesterase effect, were determined by HPLC, and the water extract was found to possess the highest caffeine content.Publication Metadata only Antioxidant and anticholinesterase activities of eleven edible plants(2011-03-01T00:00:00Z) Boga, Mehmet; Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILObjective: The antioxidant potential and anticholinesterase activity of eleven edible plants were investigated.Publication Open Access Screening antioxidant and anticholinesterase potential of Iris albicans extracts(2015-03-01) Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILThe antioxidant and anticholinesterase activities of the extracts prepared from the rhizomes and flowering aerial parts of Iris albicans were determined in this study. The chloroform extract of the rhizomes was rich in total phenolic contents (431.98 +/- 0.49 mu gPEs/mg), and the chloroform extract of the aerial parts in total flavonoid contents (663.05 +/- 0.32 mu gQEs/mg). Although the chloroform extract of the rhizomes exhibited the best antioxidant effect in beta-carotene bleaching and CUPRAC methods among the tested extracts at all concentrations, it was found inactive in the metal chelating assay. The methanol extract of the aerial parts indicated moderate metal chelating activity (60%) at 100 mu g/mL. The chloroform extract of the rhizomes showed moderate anticholinesterase effect at 200 mu g/mL. The chloroform extract of the aerial parts showed significantly inhibition against butyrylcholinesterase (78.44 +/- 0.51%). (C) 2012 Production and hosting by Elsevier B. V. on behalf of King Saud University.Publication Metadata only In vitro biological activities and fatty acid profiles of Pistacia terebinthus fruits and Pistacia khinjuk seeds(2015-03-04) Hacibekiroglu, IŞIL; Yilmaz, Pelin Koseoglu; Hasimi, Nesrin; Kilinc, Ersin; Tolan, Veysel; Kolak, Ufuk; GAZİOĞLU, IŞILThis study reports in vitro anticholinesterase, antioxidant and antimicrobial effects of the n-hexane, dichloromethane, ethanol and ethanol-water extracts prepared from Pistacia terebinthus L. fruits and Pistacia khinjuk Stocks seeds as well as their total phenolic and flavonoid contents, and fatty acid compositions. Ethanol and ethanol-water extracts of both species exhibited higher anticholinesterase activity than galanthamine. Among ABTS, DPPH and CUPRAC assays, the highest antioxidant capacity of the extracts was found in the last one. P. terebinthus ethanol extract being rich in flavonoid content showed the best cupric reducing effect. All extracts possessed no antimicrobial activity. The main fatty acid in P. terebinthus fruits (52.52%) and P. khinjuk seeds (59.44%) was found to be oleic acid. Our results indicate that P. terebinthus fruits and P. khinjuk seeds could be a good source of anticholinesterase compounds, and could be phytochemically investigated.Publication Metadata only Antioxidant and Anticholinesterase Constituents from the Petroleum Ether and Chloroform Extracts of Iris suaveolens(2011-04-01T00:00:00Z) Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILThe aim of this study was to investigate the phytochemical, antioxidant and anticholinesterase activities of Iris suaveolens. After determining total phenolic and flavonoid contents of the petroleum ether, chloroform and methanol extracts prepared from the rhizomes, the antioxidant capacity of the extracts was established using beta-carotene-linoleic acid and CUPRAC methods. The chloroform extract which was rich in phenolic content exhibited the highest inhibition of lipid peroxidation in the beta-carotene-linoleic acid system, and the best cupric reducing antioxidant capacity among the tested extracts. The petroleum ether extract indicated moderate anticholinesterase activity while the chloroform extract revealed significant butyrylcholinesterase inhibitory activity (75.03 +/- 1.29%). Spectroscopic methods were used for the structural elucidation of the compounds (1-13) isolated from the petroleum ether and chloroform extracts. Coniferaldehyde (6), having the highest antioxidant activity in the beta-carotene-linoleic acid assay at 25 and 50 mu g/mL, demonstrated also the best effect in the CUPRAC method among the tested compounds (1-12). 3-Hydroxyirisquinone (10) showed the best anticholinesterase activity among the tested compounds (1-4, 6-12), and coniferaldehyde exhibited almost the same butyrylcholinesterase inhibitory activity (82.60 +/- 2.33%) as galantamine (86.26 +/- 0.66%). Copyright (C) 2010 John Wiley & Sons, Ltd.Publication Metadata only A potential therapeutic role in multiple sclerosis for stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata(2017-07-01) Acar, Ozden Ozgun; GAZİOĞLU, IŞIL; Kolak, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIMultiple sclerosis (MS) is an autoimmune disease of the human central nervous system. It is one of the most common neurological disorders around the world and there is still no complete cure for MS. Purification of a terpenoid from Capparis ovata was carried out and its structure was elucidated as stigmast-5,22-dien-3 beta-ol, myristate (3 beta, 22E-stigmasteryl myristate; SDM) by NMR and mass spectral analyses. No information regarding its any health effect is available in the literature. In the present study, we have described its effects on inflammatory factors such as the expression levels of cytokines, chemokines and adhesion molecules as well as apoptosis/infiltration and myelination in SH-SY5Y cells. The expression levels of proinflammatory or inflammatory cytokines and chemokines such as NF-.B1, CCL5, CXCL9, CXCL10 and HIF1A along with T-cell activating cytokines such as IL-6 and TGFB1 were significantly downregulated with SDM treatment. Moreover, the expression levels of the main myelin proteins such as MBP, MAG and PLP that are essential for healthy myelin architecture were significantly up-regulated. The results presented in this study strongly suggest that the SDM offers a unique possibility to be used with autoimmune diseases, including MS due to its activity on the manipulation of cytokines and the promotion of myelin formation.Publication Metadata only Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice(2016-09-15) Ozgun-Acar, Ozden; Celik-Turgut, Gurbet; Gazioglu, IŞIL; Kolak, Ufuk; Ozbal, Seda; Ergur, Bekir U.; Arslan, Sevki; Sen, Alaattin; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTISince ancient times, Capparis species have been widely used in traditional medicine to treat various diseases. Our recent investigations have suggested Capparis ovata-s potential anti-neuroinflammatory application for the treatment of multiple sclerosis (MS). The present study was designed to precisely determine the underlying mechanism of its anti-neuroinflammatory effect in a mouse model of MS. C. ovata water extract (COWE) was prepared using the plant-s fruit, buds, and flower parts (Turkish Patent Institute, PT 2012/04,093). We immunized female C57BL/6 J mice with MOG(35_55)/CFA. COWE was administered at a daily dose of 500 mg/Kg by oral gavage either from the day of immunization (T1) or at disease onset (12) for 21 days. Gene expression analysis was performed using a Mouse Multiple Sclerosis RT2 Profiler PCR Array, and further determinations and validations of the identified genes were performed using qPCR. Whole-genome transcriptome profiling was analyzed using Agilent SurePrint G3 Mouse GE 8X60K microarrays. Immunohistochemical staining was applied to brain sections of the control and treated mice to examine the degree of degeneration. COWE was further fractionated and analyzed phytochemically using the Zivak Tandem Gold Triple Quadrupole LC/MS-MS system. COWE remarkably suppressed the development of EAE in T1, and the disease activity was completely inhibited. In the T2 group, the maximal score was significantly reduced compared with that of the parallel EAE group. The COWE suppression of EAE was associated with a significantly decreased expression of genes that are important in inflammatory signaling, such as TNF alpha, IL6, NF-kappa B, CCL5, CXCL9, and CXCK10. On the other hand, the expression of genes involved in myelination/remyelination was significantly increased. Immunohistochemical analysis further supported these effects, showing that the number of infiltrating immune cells was decreased in the brains of COWE-treated animals. In addition, differential expression profiling of the transcriptome revealed that COWE treatment caused the down regulation of a group of genes involved in the immune response, inflammatory response, antigen processing and presentation, B-cell-mediated immunity and innate immune response. Collectively, these results suggest anti-neuroinflammatory mechanisms by which COWE treatment delayed and suppressed the development of EAE and ameliorated the disease in mice with persistent clinical signs. (C) 2016 Elsevier B.V. All rights reserved.Publication Metadata only Chemical Profile of Malva Neglecta and Malvella Sherardiana by LC-MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents(2017-01-01) Hasimi, Nesrin; Ertas, Abdulselam; Oral, Elif Varhan; Alkan, Huseyin; Boga, Mehmet; Yilmaz, Mustafa Abdullah; Yener, Ismail; Gazioglu, IŞIL; Ozaslan, Cumali; AKDENIZ, Mehmet; Kolak, Ufuk; GAZİOĞLU, IŞILThe purpose of the present work was to determine the phenolic and the fatty acids profiles by LC-MS/MS and GC/MS with their antioxidant, anticholinesterase and antimicrobial activities, and aflatoxin contents of Malva neglecta and Malvella sherardiana. The phenolic contents of M. neglecta and M. sherardiana were determined by LC-MS/MS. Malic and 4-OH benzoic acids were found to be the most abundant compounds in M. neglecta and M. sherardiana, respectively. On the other hand, essential oil and fatty acid compositions were determined by GC/MS analysis. The methanol extracts of the plants showed the highest effect in all antioxidant assays in this study. The methanol extract of M. neglecta showed the highest activity among the petroleum ether, acetone, methanol and water extracts against acetyl-and butyryl-cholinesterase enzymes (53.68% and 63.95% inhibition ratio, respectively). The acetone extracts of M. neglacta and M. sherardiana exhibited moderate activity against C. albicans with 18 and 17 mm inhibion zone diameter, respectively. The results of the present study is also showed that the M. neglecta and M. sherardiana can also be used as a food source due to its high phenolic acid content and good antioxidant property. Also, the samples were aflatoxin free.Publication Metadata only Phytochemical Investigation of Leontice leontopetalum L. subsp ewersmannii with Antioxidant and Anticholinesterase Activities(2011-01-01T00:00:00Z) Kolak, Ufuk; Hacibekiroglu, IŞIL; Boga, Mehmet; Özgökçe, Fevzi; Ünal, Murat; CHOUDHARY, M. Iqbal; Ulubelen, Ayhan; GAZİOĞLU, IŞILTwo known quinolizidine alkaloids, lupanine and leontiformidine, were isolated from the tubers of L. leontopetalum subsp. ewersmannii. Lupanine having the highest inhibition of lipid peroxidation at 100 mu g/mL among the tested samples indicated almost the same ABTS cation radical scavenging activity with BHT, alpha-tocopherol and (+)-catechin at the same concentration. Lupanine and the alkaloidal extract showed almost the same butyrylcholinesterase inhibitory activity with galantamine at 200 mu g/mL.Publication Open Access Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection(2015-07-01) Gazioglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILModified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol-water (75+25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.