Person: GAZİOĞLU, IŞIL
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Publication Open Access An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization(2017-03-01) Tekkeli, S. E. Kepekci; GAZIOGLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞILA new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.Publication Metadata only Synthesis, antioxidant activity and SAR study of novel spiro-isatin-based Schiff bases(2018-12-01) Sönmez, Fatih; ZENGİN KURT, BELMA; Küçükislamoğlu, Mustafa; Avcı, Davut; GAZİOĞLU, IŞIL; Güneşli, Zuhal; ZENGİN KURT, BELMA; GAZİOĞLU, IŞILA new series of 21 Schiff bases of spiro-isatin was synthesized, and their DPPH, CUPRAC and ABTS cation radical scavenging abilities were investigated for antioxidant activity. The results showed that all the synthesized compounds exhibited antioxidant activity for each assay. 5 & x30d;-(2,3-Dihydroxybenzylideneamino)spiro[[1,3] dioxolane-2,3 & x30d;-indoline]-2 & x30d;-on (5c) (IC50 = 4.49 mu M, for DPPH; IC50 = 0.39 mu M, for ABTS(.+); and A(0.50) = 0.42 mu M, for CUPRAC) showed significantly better ABTS, CUPRAC and DPPH radical scavenging ability than quercetin (IC50 = 8.69 mu M, for DPPH; IC50 = 15.49 mu M, for ABTS(.+); and A(0.50) = 18.47 mu M, for CUPRAC), which is used as a standard. SAR study showed that the synthesized compounds had higher ABTS(.+) activity than DPPH and CUPRAC activities. Moreover, the compounds (5c and 5d), containing two hydroxyl groups, exhibited the highest antioxidant activities for all assays. Quantum chemical calculations were also carried out to support SAR results.Publication Metadata only Synthesis, anticholinesterase activity and molecular modeling study of novel carbamate-substituted thymol/carvacrol derivatives(2017-02-15) KURT, Belma Zengin; Gazioglu, IŞIL; Dag, AYDAN; Salmas, Ramin Ekhteiari; Kayik, Gulru; Durdagi, Serdar; Sonmez, Fatih; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; DAĞ, AYDANNew thymol and carvacrol derivatives with the carbamate moiety were synthesized and their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated. 5-isopropyl-2-methylphenyl(3-fluorophenyl)carbamate (29) was found to be the most potent AChE inhibitor with IC50 values of 2.22 mu M, and 5-isopropyl-2-methylphenyl (4-fluorophenyl)carbamate (30) exhibited the strongest inhibition against BuChE with IC50 value of 0.02 mu M. Additionally, the result of H4IIE hepatoma cell toxicity assay for compounds 18, 20, 29, 30 and 35 showed negligible cell death at 0.07-10 mu M. Moreover in order to better understand the inhibitory profiles of these molecules, molecular modeling studies were applied. Binding poses of studied compounds at the binding pockets of AChE and BuChE targets were determined. Predicted binding energies of these compounds as well as structural and dynamical profiles of molecules at the target sites were estimated using induced fit docking (IFD) algorithms and post-processing molecular dynamics (MD) simulations methods (i.e., Molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) approaches). (C) 2016 Elsevier Ltd. All rights reserved.Publication Metadata only Identification of Biotransformed Products of Some Terpenoids by Aspergillus flavus, A. niger and Cunnignhamella elegans(2018-04-28) GAZİOĞLU, IŞIL; GAZİOĞLU, IŞILPublication Open Access Design, synthesis and docking study of novel coumarin ligands as potential selective acetylcholinesterase inhibitors(2017-01-01) Sonmez, Fatih; KURT, Belma Zengin; Gazioglu, IŞIL; BASILE, Livia; Dag, AYDAN; CAPPELLO, Valentina; GINEX, Tiziana; Kucukislamoglu, Mustafa; GUCCIONE, Salvatore; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; DAĞ, AYDANNew coumaryl-thiazole derivatives with the acetamide moiety as a linker between the alkyl chains and/or the heterocycle nucleus were synthesized and in vitro tested as acetylcholinesterase (AChE) inhibitors. 2-(diethylamino)-N-(4-(2-oxo-2H-chromen-3-yl)thiazol-2-yl)acetamide (6c, IC50 value of 43 nM) was the best AChE inhibitor with a selectivity index of 4151.16 over BuChE. Kinetic study of AChE inhibition revealed that 6c was a mixed-type inhibitor. Moreover, the result of H4IIE hepatoma cell toxicity assay for 6c showed negligible cell death. Molecular docking studies were also carried out to clarify the inhibition mode of the more active compounds. Best pose of compound 6c is positioned into the active site with the coumarin ring wedged between the residues of the CAS and catalytic triad of AChE. In addition, the coumarin ring is anchored into the gorge of the enzyme by H-bond with Tyr130.Publication Metadata only Comparison of antioxidant, anticholinesterase, and antidiabetic activities of three curcuminoids isolated from Curcuma longa L.(2017-01-01) Kalaycıoğlu, Zeynep; Gazioglu, IŞIL; Erim, F. Bedia; GAZİOĞLU, IŞILAntioxidant, anticholinesterase and antidiabetic activities of three curcuminoids isolated from the Curcuma longa were simultaneously tested and compared in this study. The highest antioxidant power was detected for curcumin with the applied methods. The drug potentials of curcuminoids for Alzheimer-s disease were controlled. Bisdemethoxycurcumin (BDMC) showed substantial inhibitory activity. The activity of demethoxycurcumin (DMC) followed BDMC, whereas curcumin showed very little acetylcholinesterase inhibition activity. Antidiabetic activity of curcuminoids was evaluated by their -glucosidase inhibitory activities. All curcuminoids show activities with decreasing order as BDMC>curcumin>DMC. The significant activities of BDMC compared to its isomers and examination of chemical structures of isomers might be a starting point in designing new drugs for Alzheimer-s and Diabetes Mellitus.Publication Metadata only Microbial Biotransformation of a Tricyclic Antidepressant Protriptyline(2018-09-20) GAZİOĞLU, IŞIL; GAZİOĞLU, IŞILPublication Metadata only Antioxidant activities of novel eugenol derivatives substituted carbamate(2015-11-29) Yanıkoğlu, RABİA SARE; ZENGİN KURT, BELMA; GAZİOĞLU, IŞIL; Sonmez, Fatih; YANIKOĞLU, RABİA SARE; ZENGİN KURT, BELMA; GAZİOĞLU, IŞILPublication Open Access Screening antioxidant and anticholinesterase potential of Iris albicans extracts(2015-03-01) Hacibekiroglu, IŞIL; Kolak, Ufuk; GAZİOĞLU, IŞILThe antioxidant and anticholinesterase activities of the extracts prepared from the rhizomes and flowering aerial parts of Iris albicans were determined in this study. The chloroform extract of the rhizomes was rich in total phenolic contents (431.98 +/- 0.49 mu gPEs/mg), and the chloroform extract of the aerial parts in total flavonoid contents (663.05 +/- 0.32 mu gQEs/mg). Although the chloroform extract of the rhizomes exhibited the best antioxidant effect in beta-carotene bleaching and CUPRAC methods among the tested extracts at all concentrations, it was found inactive in the metal chelating assay. The methanol extract of the aerial parts indicated moderate metal chelating activity (60%) at 100 mu g/mL. The chloroform extract of the rhizomes showed moderate anticholinesterase effect at 200 mu g/mL. The chloroform extract of the aerial parts showed significantly inhibition against butyrylcholinesterase (78.44 +/- 0.51%). (C) 2012 Production and hosting by Elsevier B. V. on behalf of King Saud University.Publication Metadata only Capparis ovate ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice(2015-09-01) Ozgun, O.; Turgut, G. C.; Gazioglu, IŞIL; Kolat, U.; Ozbal, S.; Ergur, B. U.; Arslan, S.; Topcu, GÜLAÇTI; Sen, A.; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI