Person: ALİM TORAMAN, GÜLBAHAR ÖZGE
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Centaurea nemecii NAB. Ekstrelerinin Antikolinesteraz İnhibitör Aktivitesi
GC-MS and LC-MS Analyses of a Brown Algae Cladostephus spongiosus Extracts with Bioactivity Studies
Salvia sericeo-tomentosa var. sericeo-tomentosa ve Salvia sericeo-tomentosa var. hatayica taksonlarının polar ekstrelerinin kimyasal içeriği ve antidiyabetik aktivitelerinin belirlenmesi
Di-, and Triterpenoids Isolation and LC-MS Analysis of Salvia marashica Extracts with Bioactivity Studies
In this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salvia marashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of these extracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidant activity was determined by four complementary methods including inhibition of lipid peroxidation (by beta-carotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity and CUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity tests were carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. The triterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone and methanol extracts isolated by chromatographic methods. Their structures were determined based on spectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone or methanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate, lup-12, 20(29)-diene, lup-20(29)-ene, alpha-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroid beta-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene (2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in the present study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MS analysis.
Yeşil Alg Caulerpa cylindracea Sonder-den İzole Edilen Bis-İndol Alkaloidlerin SARS-CoV-2-ye Karşı in vitro Antiviral Aktivitesi
Caulerpaceae familyasına ait olanCaulerpa cylindraceaSonder yeşil bir deniz yosunudur. Gelibolu yarımadası kıyılarından SCUBA dalışlatoplananCaulerpacylindracea-nın builk kimyasal araştırması ile caulerpin1, mono metil caulerpinat2, beta-sitosterol ve palmitik asit izole edildi. İzole edilen bileşiklerin kimyasal yapıları, 1D- ve 2D-NMR ve kütle spektroskopik analizlerle aydınlatıldı. Caulerpin ve mono metil caulerpinatınin vitroanti-viral aktivitesi, Spike proteini ve main proteaza (3CL) karşı incelendi. Bu çalışmanın bulguları, caulerpin ve mono metil caulerpinatın Spike ve ACE2 etkileşimini önemli ölçüde inhibe ettiğini ortaya koymaktadır. Ayrıca, izole edilen bileşiklerin sitotoksik aktivitesi, sağlıklı fibroblast hücre hattı CCD-1079Sk ve iki meme kanseri hücre hattı; MDA-MB-231 ve MCF-7’ye karşı incelendi.
Isoquinoline alkaloids isolated from Glaucium corniculatum var. corniculatum and Glaucium grandiflorum subsp. refractum var. torquatum with bioactivity studies
Context: The genus Glaucium Mill., one of the important Papaveraceae family plants, is rich in isoquinoline alkaloids and distributed worldwide. Objective: Isolation and identification of bioactive alkaloids from Glaucium grandiflorum Boiss. & Huet. subsp. refractum (Nabelek) Mory var. torquatum (Cullen) Mory and G. corniculatum (L.) Rudolph var. corniculatum (Aslan 2012), and investigation of their antioxidant and anticholinesterase activities. Materials and methods: The aerial parts of each plant were dried, powdered, and percolated with methanol, then each extract was fractionated between 50% aqueous acetic acid and petroleum. Their aqueous acidic layer was adjusted to pH 7-8 with NH4OH and extracted with chloroform, the extract was subjected to CC separation and isolation. Structures of the isolated alkaloids were elucidated by 1D and 2D-NMR and mass spectral analyses. The alkaloid extracts and their pure alkaloids were tested for anti-cholinesterase (AChE and BuChE) and antioxidant (ABTS, CUPRAC, β-carotene linoleic acid tests) activities in vitro. Results: Methanol extracts of Glaucium grandiflorum subsp. refractum var. torquatum and G. corniculatum var. corniculatum afforded a novel compound glauciumoline and seven known isoquinoline alkaloids three of which have an aporphine-type and the other five have a protopine-type skeleton. Among them, trans-protopinium (7) and cis-protopinium (8) were isolated from a Glaucium species for the first time. Tertiary amine extracts (TAEs) of both plants showed very strong acetylcholinesterase inhibitory activity. The TAE of the plants also showed strong antioxidant activity while the isolated alkaloids showed no meaningful activity in the anticholinesterase and antioxidant tests. Discussion and conclusions: Glaucium species are considered promising therapeutic agents in the treatment of Alzheimer's disease.