Person: ÖZDER, ACLAN
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Determination of Genetic Changes of Rev-erb beta and Rev-erb alpha genes in Type 2 Diabetes Mellitus by Next-Generation Sequencing
Background: The nuclear receptors Rev-erb alpha and Rev-erb beta are transcription factors that regulate the function of genes in glucose and lipid metabolism, and they also form a link between circadian rhythm and metabolism. We evaluated the variations in Rev-erb alpha and Rev-erb beta genes together with biochemical parameters as risk factors in type 2 diabetic (T2DM) patients. Methods: Molecular analyses of Rev-erb alpha and Rev-erb beta genes were performed on genomic DNA by using next-generation sequencing in 42 T2DM patients (21 obese and 21 non-obese) and 66 healthy controls. Results: We found 26 rare mutations in the study groups, including 13 missense mutations, 9 silent mutations, 3 5'UTR variations, and a 3'UTR variation, of which 9 were novel variations (5 missense and 3 silent and 1 5'UTR). Six common variations were also found in the Rev-erb genes; Rev-erb beta Chr3:24003765 A > G, Rev-erb beta rs924403442 (Chr3:24006717) G > T, Rev-erb alpha Chr17:38253751 T > C, Rev-erb alpha rs72836608 C > A, Rev-erb alpha rs2314339 C > T and Rev-erb alpha rs2102928 C > T. Of these, Rev-erb beta Chr3:24003765 A > G was a novel missense mutation (p.Q197R), while others were identified as intronic variants. T2DM patients with Rev-erb beta rs924403442 T allele had lower body surface area (BSA) than noncarriers (GG genotype) (p = 0.039). Rev-erb alpha rs72836608 A allele and Rev-erb alpha rs2314339 CC genotype were associated with decreased serum HDL-cholesterol levels in T2DM patients (p = 0.025 and p = 0.027, respectively). In our study, different effects of Rev-erbs polymorphisms were found according to gender and presence of obesity. Rev-erb alpha rs72836608 (C > A) and rs2314339 (C > T) and Rev-erb alpha rs2102928 (C > T) were associated with low HDL-C levels in male T2DM patients. In female patients, Rev-erb alpha rs2102928 (C > T) was associated with high microalbuminuria and Rev-erb beta rs9244403442 G > T was associated with low HDL and high BSA values. In addition, Rev-erb alpha Chr17: 38,253,751 (T > C), rs72836608 (C > A), and rs2314339 (C > T) and Rev-erb beta Chr3:24003765 (A > G) were associated with increased serum GGT levels in obese T2DM patients. In non-obese patients, Rev-erbs SNPs had no effect on serum GGT levels. Conclusion: Our findings indicate that variations in the Rev-erb alpha and Rev-erb beta genes can affect metabolic changes in T2DM and these effects may vary depending on gender and obesity.
Glycosylated hemoglobin A1c predicts coronary artery disease in non-diabetic patients
Background In primary care, there is a need for simple and cost-effective tool that will allow the determination of the risk of coronary artery disease (CAD). We aimed to research the value of glycosylated hemoglobin (HbA1c) in the prediction of coronary artery disease. Methods Patients admitted to the outpatient clinic of the Cardiology for angiography were retrospectively screened. Patients with diabetes or with HbA1c of 6.5 or above were excluded. Comparative HbA1c data were obtained according to the stenosis groups. Logistic regression analysis was used to investigate the risk factors affecting stenosis positivity. Results Of the study group, 120 patients were without any stenosis in any coronary artery, 56 patients were with >50% stenosis in one coronary artery, and 71 patients were with >50% stenosis in more than one coronary artery. There was a statistically significant difference between HbA1c measurements according to the degree of stenosis (P = .001 andP < .01, respectively). The odd ratio for HbA1c was 6.260 (95% CI: 3,160-12,401). According to the stenosis positivity, the cutoff point for HbA1c was found to be 5.6 and above. In the regression analysis, HbA1c was an independent risk factor for CAD. One unit increase in HbA1c level increases the risk of stenosis up to 12.4-fold (95% CI: 5,990-25,767). Conclusion The study showed HbA1c can be used as an independent marker in determining the probability and severity of coronary artery disease in non-diabetic individuals and as a useful marker in primary care predicting CAD.
Corrigendum to ‘A novel indicator predicts 2019 novel coronavirus infection in subjects with diabetes’. [Diab. Res. Clin. Practice 166 (2020) 108294]
A novel indicator predicts 2019 novel coronavirus infection in subjects with diabetes
Aims: Diabetes mellitus (DM) is associated with significant morbidity and mortality. The disease severity in 2019 novel coronavirus (Covid 19) infection has varied from mild self-limiting flu-like illness to fulminant pneumonia, respiratory failure and death. Since DM and Covid 19 infection are closely associated with inflammatory status, mean platelet volume (MPV) was suggested to be useful in predicting Covid infection onset. This study aimed to evaluate the diagnostic role of MPV in Covid patients with diabetes. Methods: A total of 640 subjects (160 Covid patients with type 2 diabetes, 160 healthy controls, 160 patients with non-spesific infections and 160 Covid patients without type 2 diabetes) enrolled in the study. Results: MPV was significantly higher (11.21 ± 0.61 fL) as compared to the results from the last routine visits of the the same individuals with diabetes (10.59 ± 0.96 fL) (p = 0.000). Conclusions: MPV could be used as a simple and cost-effective tool to predict the Covid infection in subjects with diabetes in primary care.
The prevalence of dysmenorrhea and its effects on female university students- quality of life: what can we do in primary care?
This study aimed to estimate the prevalence of dysmenorrhea among Turkish university students studying in the field of health sciences and to evaluate the effects of the students- socio-demographic characteristics on the condition. A cross-sectional survey of 658 students was conducted by questionnaire at a health sciences university in Turkey. A structured questionnaire was used to collect data on dysmenorrhea. Health related quality of life (HRQoL) was assessed using the generic 36-Item Short Form Health Survey (SF-36), and depressive symptoms were assessed with the BDI included in the survey. A visual analogue scale was used to assess the severity of dysmenorrhea. Data were analyzed using chi-square tests and logistic regression analysis. The prevalence of dysmenorrhea was 79.7% (524 out of 658 students). Age of menarche (P = 0.001), menstrual flow (P = 0.000), drinking coffee (P = 0.001) and having a family history of dysmenorrhea were significantly associated with dysmenorrhea in a multivariable analysis. Furthermore, 42.7% of the students with dysmenorrhea missed at least one day of school and 5.3% missed at least one exam. The high prevalence of dysmenorrhea among Turkish university students is a significant health problem requiring attention, and the need for on-the-job training of primary care physicians on the management of primary dysmenorrhea should be considered.
Neutrophil lymphocyte ratio as useful predictive tool for glycaemic control in type 2 diabetes: Retrospective, single centre study in Turkey
Abstract Objective: To evaluate the inflammatory cells in the complete blood count test and their relationship with the glycaemic control in type 2 diabetes patients. Methods: The retrospective clinical study was conducted at the Department of Family Medicine, Bezmialem Vakif University, Istanbul, Turkey, from January 2015 to June 2016, and comprised adult type 2 diabetes patients- data, like complete blood count and biochemical parameters. The data was stratified according to glycated haemoglobin levels; <7% in group 1, and >7% in group 2. Data was analysed using SPSS 21. Results: Of the 320 subjects, 172(54%) were in group 1 and 148(46%) in group 2. Overall, there were 106(33%) male and 214(67%) female subjects. The mean age of the sample was 53.6±11.9 years. White blood cell count, neutrophil count and neutrophil-lymphocyte ratios were significantly higher in group 2. Red blood cell count, mean platelet volume, fasting blood glucose, triglyceride, alanine aminotransferase levels were also significantly higher in group 2 (p <0.05). Conclusion: Simple complete blood count test can be used as a cost-effective tool to monitor glycaemic regulation in type 2 diabetes patients. Keywords: Diabetes mellitus, Type 2, HbA1c, Neutrophil, Lymphocyte. (JPMA 72: 1097; 2022) DOI: https://doi.org/10.47391/JPMA.3205
FAMILY FUNCTIONING AMONG PATIENTS WITH TYPE 2 DIABETES MELLITUS IN PRIMARY CARE IN TURKEY
Introduction: To examine the effects of family functioning on glycaemic control among adults with type 2 diabetes mellitus (T2DM) in primary health care.