Person: TIRIS, GİZEM
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Publication Metadata only Çocuklarda Dikkat Eksikliği Hiperaktivite Bozukluğu Durumunda Kullanılan İlaçlar, Gıda Takviyeleri ve Fitofarmasötiklerin İncelenmesi(2021-11-17T00:00:00Z) Tekkeli, Şerife Evrim; Özbek, Emirhan; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİMPublication Metadata only High performance liquid chromatographic analysis of lercanidipine in human breast milk(2019-01-01) Tekkeli, Evrim Kepekci; GAZİOĞLU, IŞIL; TIRIS, GİZEM; Onal, Cem; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; TIRIS, GİZEMA simple, rapid, precise and accurate isocratic reversed phase HPLC method was developed and validated for the determination of lercanidipine hydrochloride in pharmaceutical tablets and spiked human breast milk. The chromatographic separation was achieved on C18 (250x4.6 mmx5 mu m) column using a mobile phase consisting of acetonitrile and phospate buffer (pH=4) (55:45, v/v) at a flow rate of 1.1 mL/min and UV detection at 237 nm. The linearity of the proposed method was investigated in the range of 1.0-40 mu g/mL (r(2)=0.9990). The method was validated in terms of accuracy, precision, reproducibility, specificity, robustness, and detection and quantification limits, in accordance with ICH guidelines. The proposed method is found as suitable for routine quantification of lercanidipine in human breast milk.Publication Metadata only A new HPLC method for the determination of Allicin and S-Allyl cysteine in garlic (Allium sativum L.) extracts(2020-09-01T00:00:00Z) BUCAK, Seden; TEKKELİ, ŞERİFE EVRİM; CEYLAN, BURHAN; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİM; TIRIS, GİZEMPublication Metadata only Evaluation of Monomer Release of Bulk Fill Composite Resins with Extended Light Curing(2022-04-22T00:00:00Z) Tırıs, Gizem; Dönmez, Nazmiye; Dalkılıç, Evrim; ÖZDUMAN, ZÜMRÜT CEREN; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİM; DÖNMEZ, NAZMİYE; DALKILIÇ, EVRIMPublication Metadata only Ultra fast liquid chromatographic analysis of nonsteroidal anti-inflammatory drugs with fluorimetric detection in tap water, urine, and pharmaceutical samples(2022-06-01T00:00:00Z) Onal, Cem; TIRIS, GİZEM; Tekkeli, Evrim Kepekci; Onal, Armagan; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİMA novel analytical method based on ultra-fast liquid chromatography using fluorimetric detector was developed and validated for determination of non-steroidal anti-inflammatory drugs (NSAIDs) (ibuprofen (IBP), etodolac (ETD), dexketoprofen (DKP), sodium diclofenac (SDCF), and naproxen (NPX) in tap water, urine and pharmaceutical samples. Precolumn derivatisation of targeted NSAIDs was carried out with 4-bromomethyl-7-methoxy coumarin (BrMmC) using dibenzo-18-crown-6-ether as reaction catalyst leading to the formation of a fluorescent compound. The obtained fluorescent compound of NSAIDs were measured at excitation wavelength as 325 nm, and emission wavelength of 395 nm. Optimum analytical conditions were carefully studied and improved. C18 column, with the dimensions of 4.0 x 100 mm and 3 mu m particle size, was used. Gradient elution with methanol: water 40:60; v/v (eluent A) and acetonitrile 100% (eluent B) were used as mobile phase and flow rate of 0.4 mL/min. The linearity range of the analytes were between 0.01-10.0 mu g mL(-1). Recovery values obtained from pharmaceutical preparations were found as 100.04%, 99.99%, 100.09%, 99.98% and 100.47% for IBP, ETO, DKP, SDCF, NPX, respectively. LOD values were found to vary between 0.00009 mu g mL(-1) and 0.00048 mu g mL(-1) in tap water, urine and pharmaceutical samples. The optimised technique was successfully applied for the determination of NSAIDs in tap water, urine, and pharmaceutical specimen. The specified NSAIDs were not found in real tap water samples.Publication Metadata only A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study(2021-09-01T00:00:00Z) TEKKELİ, ŞERİFE EVRİM; Ceylan, Burhan; TIRIS, GİZEM; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİMThis article aims to present a simple and sensitive, HPLC-UV method, which was developed to determine carnosol in human plasma samples. Chromatographic separation was achieved with C18 column (150 mm x 4.6 mm x 5 mu m), at 25 degrees C with gradient elution of the mobile phase consisting of methanol-water (2% o-phosphoric acid) at flow rate 1.2 mL/min. The analyte was detected at 230 nm by UV detector. The retention time of carnosol is 3.40 +/- 0.01 min. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 1-20 ng/mL with the correlation coefficient of 0.9942. The proposed method was applied successfully to the analysis of carnosol in spiked human plasma with good recovery as 96.4% and the precision of the method was determined by intra day and interday assays with the highest RSD % values 5.71. The method successfully applied to a pharmacokinetic study with determination of C-max, t(max), t(1/2) and AUC, by administration of carnosol to a healthy volunteer.