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ÖZMAN, ZEYNEP

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ZEYNEP

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ÖZMAN

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Now showing 1 - 10 of 10
  • Publication
    Circulating furin, IL-6, and presepsin levels and disease severity in SARS-CoV-2-infected patients
    (2021-06-01T00:00:00Z) Kocyigit, Abdurrahim; Sogut, Ozgur; Kanimdan, Ebru; Guler, Eray Metin; Kaplan, Onur; Eren, Canan; Ozman, Zeynep; Yasar, Oznur; KOÇYİĞİT, ABDÜRRAHİM; BALKAN, EZGİ; KANIMDAN, EBRU; YENİGÜN, VILDAN BETÜL; ÖZMAN, ZEYNEP
    Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a vast number of infections and deaths that deeply affect the world. When the virus encounters the host cell, it binds to angiotensin-converting enzyme 2, then the S protein of the virus is broken down by the transmembrane protease serine 2 with the help of furin, allowing the virus to enter the cell. The elevated inflammatory cytokines suggest that a cytokine storm, also known as cytokine release syndrome, may play a major role in the pathology of COVID-19. Therefore, the aim of this study is to investigate the relationship between circulating furin levels, disease severity, and inflammation in patients with SARS-CoV-2. A total of 52 SARS-CoV-2 patients and 36 healthy control participants were included in this study. SARS- CoV-2 patients were scored by the disease activity score. Serum furin, presepsin, and interleukin-6 (IL-6) levels were assessed using an enzyme-linked immunosorbent assay. The mean furin, presepsin, and IL-6 levels were significantly higher in the peripheral blood of SARS-CoV-2 compared to the controls (p < 0.001). There were close positive relationship between serum furin and IL-6, furin and presepsin, and furin and disease severity (r = 0.793, p < 0001; r = 0.521, p < 0.001; and r = 0,533, p < 0.001, respectively) in patients with SARS-CoV-2. These results suggest that furin may contribute to the exacerbation of SARS-CoV-2 infection and increased inflammation, and could be used as a predictor of disease severity in COVID-19 patients.
  • Publication
    Verbascum Siniatum Ekstraktının Osteosarkom Hücreleri Üzerindeki Sitotoksik veApoptotik Etkilerinin İncelenmesi
    (2022-12-16) ÖZMAN Z.; BAŞARICI M. T.; DURMUŞ E.; KOÇYİĞİT A.; ÖZMAN, ZEYNEP; BALKAN, EZGİ; KOÇYİĞİT, ABDÜRRAHİM
  • Publication
    Role of Propolis in Cancer Chemoprevention
    (2024-03-03) KOÇYİĞİT A.; DURMUŞ E.; ÖZMAN Z.; KOÇYİĞİT, ABDÜRRAHİM; BALKAN, EZGİ; ÖZMAN, ZEYNEP
  • Publication
    \"PROPOLİSİN KOLOREKTAL KANSERDE 5-FLUOROURASİLİN ETKİNLİĞİNİ ARTIRMASI VE YAN ETKİLERİNİ DÜŞÜRMESİ ÜZERİNE OLAN IN VITRO VE IN VIVO BİR ÇALIŞMA\"
    (2022-12-01) Balkan E.; Özman Z.; Koçyiğit A.; BALKAN, EZGİ; ÖZMAN, ZEYNEP; KOÇYİĞİT, ABDÜRRAHİM
    Giriş ve amaç: Kolorektal kanser tedavisinde kullanılan geleneksel yöntemler yetersiz olduğundan alternatif tedavi arayışları devam etmektedir. Bu çalışma, 5-florourasil (5-FU) ve Anadolu propolis ekstraktının (PE) kombine tedavisinin CRC üzerindeki anti-tümör etkilerini ve etki mekanizmasını hem in vitro hem de in vivo çalışmalarla incelemeyi amaçlamaktadır. Deneysel prosedür: Lusiferaz ile transfekte edilmiş LoVo hücreleri (LoVo-Luc) ve sağlıklı kolon hücreleri (CCD-18Co), 24 saat boyunca 5-FU ve PE\"nin farklı konsantrasyonlarına ve bunların kombinasyonlarına maruz bırakıldı. İnkübasyondan sonra genotoksik, apoptotik sitotoksik etkiler ve hücre içi reaktif oksijen türleri (iROS) seviyeleri değerlendirildi. İn vivo anti-tümör etkilerini görselleştirmek için LoVo hücreleri, lusiferaz geni (LoVo-Luc) ile transfekte edildi ve çıplak farelerde kanser, kserografik olarak indüklendi. Tümör oluşumundan sonra, çıplak fareler PE, 5-FU ve bunların kombinasyonları ile tedavi edildi. Anti-tümör etkileri canlı hayvan görüntüleme sistemi, histopatolojik ve biyokimyasal yöntemlerle analiz edildi. Sonuçlar: İn vitro bulgular, PE\"nin LoVo ve CCD-18Co hücrelerinde 5-FU\"nun genotoksik, apoptotik ve sitotoksik etkilerini güçlendirdiğini ve bu aktivitelerin bu ajanın Iros oluşturucu etkileri ile ilişkili olduğunu gösterdi. Kanser hücreleri, özellikle daha yüksek dozlarda, bu kombinasyon tedavisine sağlıklı hücrelerden daha duyarlıydı. Fare ksenograft modelinden elde edilen in vivo veriler, PE&#39;nin intraperitoneal (IP) uygulamasının, yan etkilerini azaltarak 5-FU&#39;nun kolon CRC&#39;sine karşı anti-tümör etkinliğini arttırdığını açıkça göstermiştir. IP uygulamasıyla karşılaştırıldığında, oral yoldan verilen PE&#39;nin, büyük olasılıkla absorpsiyon başarısızlığına bağlı olarak daha az etkili olduğu gösterilmiştir. Sonuç: Sonuçlar, PE\"nin tümörde doza bağımlı bir şekilde iROS oluşturucu etki ile 5-FU&#39;nun etkinliğini arttırırken yan etkileri azalttığını göstermektedir. Bu nedenle, CRC için ek tedavi olarak kabul edilebilir.
  • Publication
    Olive Leaf Extract Downregulates the Protein Expression of Key SARS-CoV-2 Entry Enzyme ACE-2, TMPRSS2, and Furin.
    (2024-06-04) Kocyigit A.; Kanimdan E.; Yenigun V. B.; Ozman Z.; Balıbey F. B.; Durmuş E.; Yasar O.; KOÇYİĞİT, ABDÜRRAHİM; YAŞAR, ÖZNUR; KANIMDAN, EBRU; YENİGÜN, VILDAN BETÜL; ÖZMAN, ZEYNEP; BABALI BALIBEY, FATMANUR
    Severe acute respiratory syndrome coronavirus 2 poses ongoing global health challenges due to its propensity for mutations, which can undermine vaccine efficacy. With no definitive treatment available, urgent research into affordable and biocompatible therapeutic agents is extremely urgent. Angiotensin converting enzyme-2 (ACEII), transmembrane protease serine subtype 2 (TMPRSS2), and Furin enzymes, which allow the virus to enter cells, are particularly important as potential drug targets among scientists. Olive leaf extract (OLE) has garnered attention for its potential against COVID-19, yet its mechanism remains understudied. In this study, we aimed to investigate the effects of OLE on ACEII, TMPRSS2, and Furin protein expressions by cell culture study. Total phenol, flavonoid content, and antioxidant capacity were measured by photometric methods, and oleuropein levels were measured by liquid LC-HR-MS. Cell viability was analyzed by ATP levels using a luminometric method. ACEII, TMPRSS2, and Furin expressions were analyzed by the Western Blotting method. ACEII, TMPRSS2, and Furin protein expression levels were significantly lower in dose dependent manner and the highest inhibition was seen at 100 ug/ml OLE. The results showed that OLE may be a promising treatment candidate for COVID-19 disease. However, further studies need to be conducted in cells co-infected with the virus.
  • Publication
    Investigation of the Anti-inflammatory Effect of Liquidambar orientalis Leaf Extract on RAW 264.7 Macrophage Cells
    (2024-03-02) Şahin H.; Özman Z.; Koçyiğit A.; ÖZMAN, ZEYNEP; KOÇYİĞİT, ABDÜRRAHİM
  • Publication
    Verbascum sinuatum extract induces cell death in K7M2 osteosarcoma cells by its immunomodulatory effect on LPS-stimulated RAW 264.7 cells
    (2023-05-18) ÖZMAN Z.; DURMUŞ E.; BAŞARICI M. T.; KOÇYİĞİT A.; ÖZMAN, ZEYNEP; BALKAN, EZGİ; KOÇYİĞİT, ABDÜRRAHİM
  • Publication
    PÜREN VE MANUKA BALLARININ MİDE KANSER HÜCRELERİ ÜZERİNDEKİ SİTOTOKSİK ETKİLERİNİN ARAŞTIRILMASI
    (2022-12-16) Özman Z.; Babalı Balıbey F.; Balkan E.; Koçyiğit A.; ÖZMAN, ZEYNEP; BABALI BALIBEY, FATMANUR; BALKAN, EZGİ; KOÇYİĞİT, ABDÜRRAHİM
  • Publication
    Comparative cell viability of dentin-bonding adhesive systems on human dental pulp stem cells: time-dependent analysis
    (2024-12-01) Kazak M.; Sarialioglu Gungor A.; ÖZMAN Z.; Donmez N.; ÖZMAN, ZEYNEP
    Background: Restorative materials are in prolonged contact with living tissues such as oral mucosa, dentin, pulp, periodontal, and periapical tissues. Therefore, the potentially harmful effects of these materials and their components on oral tissues should be evaluated before clinical use. This study aimed to compare the cell viability of different adhesive systems (ASs) on human dental pulp stem cells (hDPSCs). Methods: Three ASs that combining methacryloyloxydecyl dihydrogen phosphate (MDP) monomer with new hydrophilic amide monomers [Clearfil Universal Bond Quick(CUBQ), Kuraray Noritake], self-reinforcing 3D monomer [Bond Force II(BFII), Tokuyama)], and dual-cure property [Futurabond DC(FBDC), VOCO] were used. Three (n = 3) samples were prepared for each group. Dental pulp stem cells were isolated from ten patients' extracted third molar teeth. Samples were incubated in Dulbecco's modified Eagle's medium (DMEM) for 24 h (h), 72 h, and 7 days (d) to obtain extracts. For the control group, cells were cultured without DBA samples. Cell viability of ASs extracts was measured using a cell proliferation detection kit (WST-1, Roche). Statistical analysis was performed using two-way ANOVA and post-hoc (Duncan) tests (p < 0.05). Results: At 24 and 72 h statistically significant differences were determined between control and BFII, control and FBDC groups (p < 0.05), while no differences between control and CUBQ groups (p > 0.05). On the 7th d, statistically significant differences were found between the control and experimental groups (p < 0.05), while no differences between experimental groups (p > 0.05). A statistically significant difference was detected for the BFII group over the three-time interval (p < 0.05). The lowest cell viability was observed for the FBDC group at 24 h, and the difference was statistically significant when compared with 72 h and 7th d (p < 0.05). Conclusion: All ASs showed different cell viability values at various exposure times. It should be taken into consideration that pH values, as well as the contents of ASs, have a significant effect on the cell viability.
  • Publication
    KRİSİNİN MCF-7 VE MDA-MB-231 MEME KANSERİ HÜCRELERİNE OLAN İMMÜN SİSTEM ARACILI SİTOTOKSİK YANIT ÜZERİNDEKİ ETKİSİNİN ARAŞTIRILMASI
    (2023-12-04) Durmuş E.; Özman Z.; Ceyran İ. H.; Koçyiğit A.; BALKAN, EZGİ; ÖZMAN, ZEYNEP; KOÇYİĞİT, ABDÜRRAHİM