Person: ULUSOY, ŞEYMA
Status
Email Address
Birth Date
Search Results
Variations in Chemical Compositions and Biological Activities of Artemisia vulgaris L. (Common Mugwort) Essential Oils at Different Growth Stages
Chemical Composition of Different Parts of the Vitex agnus-castus L. Essential Oils and Their In-Vitro Cytotoxic Activities
The essential oil (EO) compositions and chemotypes of the important ethnomedicinal plantVitex agnus-castusL. flowers, leaves, and fruits collected from Türkiye (Balıkesir and İstanbul) were identified in the present study. Different parts of theV. agnus-castusEO’sin-vitrocytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits ofV. agnus-castuswere analyzed by GC–FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of theV. agnus-castus. EOs extracted from Balıkesir region were defined as the \"α-pinene-1,8-cineole\" chemotype, while EOs extracted from İstanbul region were defined as the \"sabinene-1,8-cineole\" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze thein-vitrocytotoxic effects of flowers. This study is also the first to show thein-vitrocytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balıkesir region’s EOs were observed as more potent -especially the purple flower’s IC50 is about 4.68 µg/mL on the MCF7 cell line- than İstanbul regions, which might be attributed to the higher amount ofα-pinene, caryophyllene, and limonene content. Our results indicated that theV. agnus-castusEOs, which containα-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.
Pektinin Kimyasal Yapısı, Özellikleri ve Ekonomik Önemi: Eczacılık ve Kozmetikte Kullanımı
Development of phytoactive essential oils-composed organogels against oral pathogens
Gingivitis, one of the most common periodontal diseases, is the inflammation of the gingival tissues that causes swelling, tenderness, redness and bleeding. The treatment should contain anti-inflammatory, antibacterial, and antioxidant agents to decrease the risk of dental plaque formation and periodontal tissue damage in the inflammatory process. In line with the antibacterial, antifungal, and antioxidant effects of the geranium (Pelargonium graveolens L\"Hér.) herb, common sage (Salvia officinalis L.) herb, and mastic tree (Pistacia lentiscus L.) leaf, in this work we propose their use as active pharmaceutical ingredients of periodontal gels. The organogel formulations were produced either by microwave-assisted irradiation method or by the conventional approach (microwave-free), and further characterized for their physicochemical properties, antimicrobial efficacy and cell viability. Among them, the gel formulation containing 4 % geranium essential oil obtained by the conventional method was identified as the best candidate to be developed as an effective and safe alternative suitable for oromucosal applications.
Phytoactive essential oils-composed water-free organogels: Development, characterization and proof of antibacterial activity
The objective of this study was to develop and analyze a gel-like formulation that does not contain water, made from natural compounds found in essential oils, which are known for their antimicrobial properties and ability to reduce inflammation. Lentisk tree (Pistacia lentiscus L.), yarrow (Achillea millefolium L.) and myrtle (Myrtus communis L.) grow in the mild climate of Eurasia and are receiving particular attention for their medicinal and aromatic value. Two distinct concentrations of selected essential oils (EOs) were tested, dispersed in organogels based on 3 % (m/V) Carbopol 974P NF in PEG 400. Prior to formulation development, selected EOs were characterized by mass spectroscopy to identify the dominating bioactives. A total of 32 compounds were quantified for lentisk leaf EO, 42 for yarrow EO, and 26 for myrtle leaf EO. The best formulations were found for the highest concentration of EOs (4 %, m/m) into Carbopol/PEG400 organogels. Organogels were produced by high-speed homogenization followed by microwave heating and showed acceptable pH between 5.8 and 6.0, being compatible with the skin, with an electrical conductivity highest for yarrow EO-based organogel (YIG4%, ∼64 mV), and the lowest for myrtle EO-based organogel (MIG4%, ∼48 mV). Texture analysis showed the highest adhesion properties for lentisk EO-based organogel (LIG4%), attributed to its higher viscosity recorded for the two tested temperatures (25 °C vs 37 °C). Rheological analysis confirmed that organogels have pseudoplastic behaviour. The antibacterial activity was compared between plain EOs and EOs-based organogels, with the yarrow EO and its organogel (YIG4%) depicting the highest activity against both tested bacterial strains (S. aureus and C. acnes). On the other hand, MIG4% contributed for the highest cell proliferation rate tested in a mouse embryo fibroblast cell line (3T3-L1), whereas with LIG4% the lowest viability value was recorded.