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03 - Sağlık ve Kaliteli Yaşam

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AMAÇ 3: SAĞLIKLI BİREYLER Çocuk ölüm oranlarının azaltılması, anne sağlığının iyileştirilmesi, HIV/AIDS, sıtma ve diğer hastalıklar ile mücadelede büyük aşama kaydetmiş durumdayız. 1990 yılından bu yana, önlenebilir çocuk ölümlerinde dünya genelinde %50’yi aşan azalma olmuştur. Anne ölümleri de dünya genelinde %45 azalmıştır. 2000 ile 2013 arasında HIV/AIDS bulaşma oranı %30 azalmış, 6,2 milyonu aşkın insan sıtmadan kurtarılmıştır. Bu ölümler; önleme ve tedavi, eğitim, aşı kampanyaları, cinsel ve üreme sağlığı hizmetleri vasıtasıyla önlenebilir. Sürdürülebilir Kalkınma Amaçları; AIDS, verem, sıtma ve diğer bulaşıcı hastalık salgınlarını 2030 yılına kadar ortadan kaldırmaya yönelik cesur bir taahhüttür. Amaç, herkesin genel sağlık hizmeti, güvenli ve erişilebilir ilaç ve aşıya kavuşmasını sağlamaktır. Aşı araştırma ve geliştirmelerinin desteklenmesi, bu sürecin vazgeçilmez bir parçasıdır.

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Author: KOÇYİĞİT, ABDÜRRAHİM × End date: 2024 × Start date: 2020 × Rights: info:eu-repo/semantics/openAccess ×

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Now showing 1 - 10 of 12
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    Handbook of Oxidative Stress in Cancer
    (2022-01-01T00:00:00Z) Güler, Eray Metin; KOÇYİĞİT, ABDÜRRAHİM
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    PublicationOpen Access
    Inhibition of Carbonic Anhydrase IX Promotes Apoptosis through Intracellular pH Level Alterations in Cervical Cancer Cells
    (2021-06-01T00:00:00Z) Temiz, Ebru; Koyuncu, Ismail; Durgun, Mustafa; Caglayan, Murat; Gonel, Ataman; Güler, Eray Metin; KOÇYİĞİT, ABDÜRRAHİM; Supuran, Claudiu T.; KOÇYİĞİT, ABDÜRRAHİM
    Carbonic anhydrase IX (CAIX) is a hypoxia-related protein that plays a role in proliferation in solid tumours. However, how CAIX increases proliferation and metastasis in solid tumours is unclear. The objective of this study was to investigate how a synthetic CAIX inhibitor triggers apoptosis in the HeLa cell line. The intracellular effects of CAIX inhibition were determined with AO/EB, AnnexinV-PI, and γ-H2AX staining; measurements of intracellular pH (pHi), reactive oxygen species (ROS), and mitochondrial membrane potential (MMP); and analyses of cell cycle, apoptotic, and autophagic modulator gene expression (Bax, Bcl-2, caspase-3, caspase-8, caspase-9, caspase-12, Beclin, and LC3), caspase protein level (pro-caspase 3 and cleaved caspase-3, -8, -9), cleaved PARP activation, and CAIX protein level. Sulphonamide CAIX inhibitor E showed the lowest IC50 and the highest selectivity index in CAIX-positive HeLa cells. CAIX inhibition changed the morphology of HeLa cells and increased the ratio of apoptotic cells, dramatically disturbing the homeostasis of intracellular pHi, MMP and ROS levels. All these phenomena consequent to CA IX inhibition triggered apoptosis and autophagy in HeLa cells. Taken together, these results further endorse the previous findings that CAIX inhibitors represent an important therapeutic strategy, which is worth pursuing in different cancer types, considering that presently only one sulphonamide inhibitor, SLC-0111, has arrived in Phase Ib/II clinical trials as an antitumour/antimetastatic drug.
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    PublicationOpen Access
    Circulating furin, IL-6, and presepsin levels and disease severity in SARS-CoV-2-infected patients
    (2021-06-01T00:00:00Z) Kocyigit, Abdurrahim; Sogut, Ozgur; Kanimdan, Ebru; Guler, Eray Metin; Kaplan, Onur; Eren, Canan; Ozman, Zeynep; Yasar, Oznur; KOÇYİĞİT, ABDÜRRAHİM; BALKAN, EZGİ; KANIMDAN, EBRU; YENİGÜN, VILDAN BETÜL; ÖZMAN, ZEYNEP
    Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a vast number of infections and deaths that deeply affect the world. When the virus encounters the host cell, it binds to angiotensin-converting enzyme 2, then the S protein of the virus is broken down by the transmembrane protease serine 2 with the help of furin, allowing the virus to enter the cell. The elevated inflammatory cytokines suggest that a cytokine storm, also known as cytokine release syndrome, may play a major role in the pathology of COVID-19. Therefore, the aim of this study is to investigate the relationship between circulating furin levels, disease severity, and inflammation in patients with SARS-CoV-2. A total of 52 SARS-CoV-2 patients and 36 healthy control participants were included in this study. SARS- CoV-2 patients were scored by the disease activity score. Serum furin, presepsin, and interleukin-6 (IL-6) levels were assessed using an enzyme-linked immunosorbent assay. The mean furin, presepsin, and IL-6 levels were significantly higher in the peripheral blood of SARS-CoV-2 compared to the controls (p < 0.001). There were close positive relationship between serum furin and IL-6, furin and presepsin, and furin and disease severity (r = 0.793, p < 0001; r = 0.521, p < 0.001; and r = 0,533, p < 0.001, respectively) in patients with SARS-CoV-2. These results suggest that furin may contribute to the exacerbation of SARS-CoV-2 infection and increased inflammation, and could be used as a predictor of disease severity in COVID-19 patients.
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    \"PROPOLİSİN KOLOREKTAL KANSERDE 5-FLUOROURASİLİN ETKİNLİĞİNİ ARTIRMASI VE YAN ETKİLERİNİ DÜŞÜRMESİ ÜZERİNE OLAN IN VITRO VE IN VIVO BİR ÇALIŞMA\"
    (2022-12-01) Balkan E.; Özman Z.; Koçyiğit A.; BALKAN, EZGİ; ÖZMAN, ZEYNEP; KOÇYİĞİT, ABDÜRRAHİM
    Giriş ve amaç: Kolorektal kanser tedavisinde kullanılan geleneksel yöntemler yetersiz olduğundan alternatif tedavi arayışları devam etmektedir. Bu çalışma, 5-florourasil (5-FU) ve Anadolu propolis ekstraktının (PE) kombine tedavisinin CRC üzerindeki anti-tümör etkilerini ve etki mekanizmasını hem in vitro hem de in vivo çalışmalarla incelemeyi amaçlamaktadır. Deneysel prosedür: Lusiferaz ile transfekte edilmiş LoVo hücreleri (LoVo-Luc) ve sağlıklı kolon hücreleri (CCD-18Co), 24 saat boyunca 5-FU ve PE\"nin farklı konsantrasyonlarına ve bunların kombinasyonlarına maruz bırakıldı. İnkübasyondan sonra genotoksik, apoptotik sitotoksik etkiler ve hücre içi reaktif oksijen türleri (iROS) seviyeleri değerlendirildi. İn vivo anti-tümör etkilerini görselleştirmek için LoVo hücreleri, lusiferaz geni (LoVo-Luc) ile transfekte edildi ve çıplak farelerde kanser, kserografik olarak indüklendi. Tümör oluşumundan sonra, çıplak fareler PE, 5-FU ve bunların kombinasyonları ile tedavi edildi. Anti-tümör etkileri canlı hayvan görüntüleme sistemi, histopatolojik ve biyokimyasal yöntemlerle analiz edildi. Sonuçlar: İn vitro bulgular, PE\"nin LoVo ve CCD-18Co hücrelerinde 5-FU\"nun genotoksik, apoptotik ve sitotoksik etkilerini güçlendirdiğini ve bu aktivitelerin bu ajanın Iros oluşturucu etkileri ile ilişkili olduğunu gösterdi. Kanser hücreleri, özellikle daha yüksek dozlarda, bu kombinasyon tedavisine sağlıklı hücrelerden daha duyarlıydı. Fare ksenograft modelinden elde edilen in vivo veriler, PE&#39;nin intraperitoneal (IP) uygulamasının, yan etkilerini azaltarak 5-FU&#39;nun kolon CRC&#39;sine karşı anti-tümör etkinliğini arttırdığını açıkça göstermiştir. IP uygulamasıyla karşılaştırıldığında, oral yoldan verilen PE&#39;nin, büyük olasılıkla absorpsiyon başarısızlığına bağlı olarak daha az etkili olduğu gösterilmiştir. Sonuç: Sonuçlar, PE\"nin tümörde doza bağımlı bir şekilde iROS oluşturucu etki ile 5-FU&#39;nun etkinliğini arttırırken yan etkileri azalttığını göstermektedir. Bu nedenle, CRC için ek tedavi olarak kabul edilebilir.
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    PublicationOpen Access
    Relationship between diabetic polyneuropathy, serum visfatin, and oxidative stress biomarkers
    (2020-07-01T00:00:00Z) GÜLER, Eray Metin; BÜYÜKAYDIN, BANU; Karaaslan, Tahsin; OLGAÇ, ATİLLA; ZORLU, MEHMET; KISKAÇ, MUHARREM; KOÇYİĞİT, ABDÜRRAHİM; BÜYÜKAYDIN, BANU; GÜLER, ERAY METİN; OLGAÇ, ATİLLA; ZORLU, MEHMET; KISKAÇ, MUHARREM; KOÇYİĞİT, ABDÜRRAHİM
    Background: Diabetic polyneuropathy is a very common complication of diabetes. Numerous studies are available in terms of pathogenesis. But examination methods with low reliability are still not standardized and generally time consuming. High-sensitive, easy-to-access methods are expected. Biochemical markers are one of the subjects of research. We aimed to discover a potential biomarker that can be used for this purpose in patients with diabetes who have not yet developed symptoms of neuropathy. Aim: To determine the place and availability of visfatin and thiol-disulfide homeostasis in this disorder. Methods: A total of 392 patients with type 2 diabetes mellitus were included in the study. The polyneuropathy clinical signs were evaluated with the Subjective Peripheral Neuropathy Screen Questionnaire and Michigan Neuropathy Screening Instrument questionnaire and examination. The biochemical parameters, oxidative stress markers, visfatin, and thiol-disulfide homeostasis were analyzed and correlated with each other and clinical signs. Results: Subjective Peripheral Neuropathy Screen Questionnaire and Michigan Neuropathy Screening Instrument questionnaire with examination scores were correlated with each other and diabetes duration (P < 0.005). Neuropathy related symptoms were present in 20.7% of the patients, but neuropathy related findings were observed in 43.9% of the patients. Serum glucose, glycated hemoglobin, and visfatin were positively correlated with each other. Also, these parameters were positively correlated with the total oxidative stress index. Total and native thiol was positively correlated with total antioxidant status and negatively with oxidant status. Inversely thiol-disulfide positively correlated with higher glucose and oxidant status and negatively with total antioxidant status (P < 0.005). There was no correlation between visfatin and thiol-disulphide (P = 0.092, r = 0.086). However, a significant negative correlation was observed between visfatin and total with native thiol (P < 0.005, r = -0.338), (P < 0.005, r = -0.448). Conclusion: Diagnosis of neuropathy is one of the issues studied in patients with diabetes. Visfatin and thiol-disulfide balance were analyzed for the first time in this study with inspiring results.
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    Integrase inhibitor-based antiretroviral treatments decrease oxidative stress caused by HIV infection.
    (2020-12-01T00:00:00Z) Okay, G; Akkoyunlu, YASEMİN; Kocyigit, A; Guler, E M; Aslan, T; KOÇYİĞİT, ABDÜRRAHİM; OKAY, GÜLAY
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    PublicationOpen Access
    In Vitro Hormetic Effect Investigation of Thymol on Human Fibroblast and Gastric Adenocarcinoma Cells.
    (2020-07-17T00:00:00Z) Günes-Bayir, A; Kocyigit, A; Guler, Eray Metin; Dadak, A; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN
    The concept of hormesis includes a biphasic cellular dose-response to a xenobiotic stimulus defined by low dose beneficial and high dose inhibitory or toxic effects. In the present study, an attempt has been made to help elucidate the beneficial and detrimental effects of thymol on different cell types by evaluating and comparing the impact of various thymol doses on cancerous (AGS) and healthy (WS-1) cells. Cytotoxic, genotoxic, and apoptotic effects, as well as levels of reactive oxygen species and glutathione were studied in both cell lines exposed to thymol (0-600 mu M) for 24 h. The results showed significant differences in cell viability of AGS compared to WS-1 cells exposed to thymol. The differences observed were statistically significant at all doses applied (P <= 0.001) and revealed hormetic thymol effects on WS-1 cells, whereas toxic effects on AGS cells were detectable at all thymol concentrations. Thymol at low concentrations provides antioxidative protection to WS-1 cells in vitro while already inducing toxic effects in AGS cells. In that sense, the findings of the present study suggest that thymol exerts a dose-dependent hormetic impact on different cell types, thereby providing crucial information for future in vivo studies investigating the therapeutic potential of thymol.
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    PublicationOpen Access
    Integrase inhibitor-based antiretroviral treatments decrease oxidative stress caused by HIV infection
    (2020-12-01T00:00:00Z) AKKOYUNLU, YASEMİN; KOÇYİĞİT, ABDÜRRAHİM; OKAY, GÜLAY; Guler, E. M.; ASLAN, TURAN; AKKOYUNLU, YASEMİN; KOÇYİĞİT, ABDÜRRAHİM; ASLAN, TURAN
    OBJECTIVE: Several chronic illnesses, including HIV infection are associated with oxidative stress. In addition to HIV itself, some antiretrovirals also increase oxidative stress while decreasing viral replication. To investigate the alterations in oxidative stress parameters and thiol-disulphide homeostasis in people living with HIV who were receiving integrase inhibitor-based antiretroviral therapy.