Publication: Mass spectrometry-based untargeted metabolomics study of non-obese individuals with non-alcoholic fatty liver disease
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Date
2023-06-01
Authors
Demirel M.
Köktaşoğlu F.
Özkan E.
Dulun Ağaç H.
Gül A. Z.
Sharifov R.
Sarıkaya U.
Başaranoğlu M.
Selek Ş.
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Abstract
Objectives: Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by the accumulation of excessive fat in the liver, which can lead to fibrosis and has an increasing prevalence. NAFLD requires non-invasive diagnostic biomarkers. While typically observed in overweight individuals, it can also occur in non-obese/non-overweight individuals. Comparative studies on non-obese NAFLD patients are scarce. This study aimed to conduct a using liquid chromatography-high resolution mass spectrometry (LC-MS/MS)-based metabolic profiling of non-obese NAFLD patients and healthy controls.
Materials and methods: The patient group consisted of 27 individuals with NAFLD, while the healthy control group included 39 individuals. Both groups were between 18 and 40 years old, had a BMI of less than 25 and had alcohol consumption less than 20 g/week for men and 10 g/week for women. Serum samples were collected and analyzed using LC-MS/MS. The data were analyzed using the TidyMass and MetaboAnalyst.
Results: The LC-MS/MS analyses detected significant changes in D-amino acid metabolism, vitamin B6 metabolism, apoptosis, mTOR signaling pathway, lysine degradation, and phenylalanine metabolism pathways in non-obese NAFLD patients. Significant changes were also observed in the metabolites D-pantothenic acid, hypoxanthine, citric acid, citramalic acid, L-phenylalanine, glutamine, and histamine-trifluoromethyl-toluidide, β-hydroxymyristic acid, DL-Lactic acid, and 3-methyl-2-oxopentanoic. Overall, the study provides valuable insights into the metabolic changes associated with non-obese NAFLD patients and can contribute to the development of non-invasive diagnostic biomarkers for NAFLD.
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Keywords
Metabolomics, metabolites, nafld, non-alcoholic fatty liver disease, non-obese
Citation
Demirel M., Köktaşoğlu F., Özkan E., Dulun Ağaç H., Gül A. Z., Sharifov R., Sarıkaya U., Başaranoğlu M., Selek Ş., "Mass spectrometry-based untargeted metabolomics study of non-obese individuals with non-alcoholic fatty liver disease", SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, cilt.0, 2023