Publication:
Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells

Simple item page
dc.contributor.authorAKBAŞ, FAHRİ
dc.contributor.authorOzaydin, Ahmet
dc.contributor.authorPolat, Erdal
dc.contributor.authorOnaran, Ilhan
dc.contributor.institutionauthorAKBAŞ, FAHRİ
dc.date.accessioned2020-06-02T20:59:10Z
dc.date.available2020-06-02T20:59:10Z
dc.date.issued2020-09-01T00:00:00Z
dc.description.abstractThe extract from larval Lucilia sericata is used clinically to promote wound healing and tissue regeneration. However, its effect and underlying mechanisms on fibroblast cells, which are involved in the wound healing process, are still poorly understood. This study aimed to examine the effects of larval secretions on dermal fibroblast activity and gene expression and to evaluate the wound healing potential of their major components. Primary rat fibroblasts were cultured and treated with larval secretions. Following the treatment, the cells were used to extract RNA for gene profiling. In addition, migration to the injury site was studied with the scratch healing assay. Our results showed that larval secretion accelerated the migration of the fibroblasts compared to the control cells and that several mRNAs were differentially expressed during a period of 72 h incubation. Additionally, we analyzed the chemical composition of larval secretions and showed that fumaric acid, ferulic acid, and p-coumaric acid, which were selected and identified for their major components, enhanced the migration of the fibroblasts. Therefore, these results indicate that L. sericata larval secretions could modulate the mRNA expression of some wound healing-related genes of the fibroblasts and contain the effective components for wound healing.
dc.description.sponsorshipBezmiâlem Vakıf Üniversitesi
dc.description.sponsorshipİstanbul Üniversitesi
dc.identifier.citationAKBAŞ F., Ozaydin A., Polat E., Onaran I., -Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells-, RECORDS OF NATURAL PRODUCTS, cilt.14, ss.340-354, 2020
dc.identifier.doi10.25135/rnp.180.20.03.1582
dc.identifier.scopus85084456830
dc.identifier.trdizindoi.org/10.25135/rnp.180.20.03.1582
dc.identifier.trdizindoi.org/10.25135/rnp.180.20.03.1582
dc.identifier.trdizintrdizin
dc.identifier.urihttps://hdl.handle.net/20.500.12645/18033
dc.identifier.wosWOS:000531162200004
dc.language.isoen
dc.titleLucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells
dc.typeArticle
dspace.entity.typePublication
local.avesis.idd7b2986b-d2e4-4467-8a34-a76de4aa563a
local.publication.isinternational1
relation.isAuthorOfPublication1da13c4a-283a-47d4-a763-1c242fde6919
relation.isAuthorOfPublication.latestForDiscovery1da13c4a-283a-47d4-a763-1c242fde6919
Files
Collections
Yayınlar - Eserler
TR Dizin İndeksli Yayınlar Koleksiyonu