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Glioblastoma Stem Cells and Comparison of Isolation Methods.

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dc.contributor.authorKaynar, MY
dc.contributor.authorDundar, TOLGA TURAN
dc.contributor.authorHatiboglu, MA
dc.contributor.authorErgul, Z
dc.contributor.authorSozen, E
dc.contributor.authorSeyithanoglu, MH
dc.contributor.authorTuzgen, S
dc.contributor.authorKaraoz, E
dc.contributor.institutionauthorDÜNDAR, TOLGA TURAN
dc.contributor.institutionauthorHATİBOĞLU, MUSTAFA AZİZ
dc.contributor.institutionauthorSEYİTHANOĞLU, MEHMET HAKAN
dc.date.accessioned2019-10-05T22:07:40Z
dc.date.available2019-10-05T22:07:40Z
dc.date.issued2019-06-01
dc.description.abstractBackground: Glioblastoma (GBM) is the most aggressive and the most common primary brain tumor. Over the last few years, studies have identified many genetical and phenotypical molecular situations for developing new treatment modalities in patients with GBM. Nevertheless, main problem for the GBM is radio-chemotherapy resistance and relapse after the surgery. The identification of glioma stem cells and microenvironmental influences has created a paradigm shift in targets of therapy. Current studies have shown that glioma stem cell is responsible for aggressiveness, recurrence and resistance to therapy of GBM. GBM stem cell isolated from human GBM multiforme fresh tissue samples is important both for curative therapeutic options and personalized targeted therapy. The purpose of this study was to determine the most suitable isolation method of GBM stem cells (GSCs). Methods: Tumor tissue sample was obtained during the surgical resection of lesion in patients with the diagnosis of GBM. Tumor stem cell isolation from tissue was performed in three different ways: 1) GBM cell isolation with trypsin; 2) GBM cell isolation with brain tumor dissociation Kit (BTD Kit); and 3) GBM cell isolation with tumor dissociation enzyme (TDE). Results: We showed that GSCs were isolated from tumor specimen using flow cytometry and immunofluorescence staining. Our study showed that isolation with BTD Kit is the most suitable method to isolate GBM tissue-derived glial tumor stem cells. Conclusions: The development of alternative personalized therapies targeting brain tumor stem cell is urgently needed. It is important to understand the fundamental mechanisms of driving stem cells. If their life cycle mechanisms can be identified, we can control the growth of GBM.en
dc.identifier10.1016/j.braindev.2014.09.001
dc.identifier.citationDundar T. T. , Hatiboglu M., Ergul Z., Seyithanoglu M., Sozen E., Tuzgen S., Kaynar M., Karaoz E., -Glioblastoma Stem Cells and Comparison of Isolation Methods.-, Journal of clinical medicine research, cilt.11, ss.415-421, 2019
dc.identifier.doi10.14740/jocmr3781
dc.identifier.pubmed31143308
dc.identifier.trdizintrdizin
dc.identifier.urihttps://hdl.handle.net/20.500.12645/8974
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleGlioblastoma Stem Cells and Comparison of Isolation Methods.
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameBRAIN & DEVELOPMENT
local.avesis.iddf3553dd-fa06-40e2-b0b4-1ea08666bb9b
local.avesis.response8850
local.publication.isinternational1
relation.isAuthorOfPublication9efe18e9-8f9c-4993-8717-39f7347f9791
relation.isAuthorOfPublicationdb1f8830-0234-4e6a-8b53-c1176e6da242
relation.isAuthorOfPublication7dd0eaf7-a02d-4124-8747-1dca94079938
relation.isAuthorOfPublication.latestForDiscoverydb1f8830-0234-4e6a-8b53-c1176e6da242
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