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Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

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dc.contributor.authorTEKKELİ, ŞERİFE EVRİM
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2020-10-22T18:54:55Z
dc.date.available2020-10-22T18:54:55Z
dc.date.issued2020-01-01T00:00:00Z
dc.description.abstractA sensitive method is presented for the determination of the ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride). Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate (pH 10) to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0-200.0 ng mL(-1), with a coefficient of determination of 0.9999 with a limit of detection (LOD) of 0.14 ng mL(-1) and the limit of quantitation (LOQ) of 0.46 ng mL(-1). The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.
dc.identifier.citationOnal C., TEKKELİ Ş. E. , -Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent-, JOURNAL OF RESEARCH IN PHARMACY, cilt.24, ss.765-773, 2020
dc.identifier.doi10.35333/jrp.2020.229
dc.identifier.urihttp://hdl.handle.net/20.500.12645/24346
dc.identifier.wosWOS:000570795700015
dc.titleSpectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent
dc.typeArticle
dspace.entity.typePublication
local.avesis.ida56b2d0d-6c5f-4719-97eb-32f072f68a78
local.publication.isinternational1
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery837d5047-762e-43db-b252-9f3d1399378f
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