Publication:
Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities.

Simple item page
dc.contributor.authorERENLER, R
dc.contributor.authorMERAL, B
dc.contributor.authorSEN, O
dc.contributor.authorELMASTAS, M
dc.contributor.authorAYDIN, A
dc.contributor.authorEMINAGAOGLU, O
dc.contributor.authorTopcu, GÜLAÇTI
dc.contributor.institutionauthorTOPÇU, GÜLAÇTI
dc.date.accessioned2019-10-05T13:40:04Z
dc.date.available2019-10-05T13:40:04Z
dc.date.issued2017-12-01
dc.description.abstractContext: Origanum (Lamiaceae) has been used in food and pharmaceutical industries. Objective: Isolation and identification of bioactive compounds from Origanum rotundifolium Boiss. and investigation of their antiproliferative and antioxidant activities. Materials and methods: The aerial part of O. rotundifolium was dried and powdered (1.0 kg ±2.0 g) then extracted with hexane, ethyl acetate, methanol and water. Solvent (3 × 1 L) was used for each extraction for a week at room temperature. The aqueous extract was partitioned with ethyl acetate (3 × 1 L) to yield the water/EtOAc extract subjected to chromatography to isolate the active compounds. The structures of isolated compounds were elucidated by 1 D, 2 D NMR and LC-TOF/MS. Results: Apigenin (1), ferulic acid (2), vitexin (3), caprolactam (4), rosmarinic acid (5), and globoidnan A (6) were isolated and identified. Globoidnan A (6), vitexin (3), and rosmarinic acid (5) revealed the excellent DPPH• scavenging effect with IC50 values of 22.4, 31.4, 47.2 μM, respectively. Vitexin (3) (IC50 3.6), globoidnan A (6) (IC50 4.6), apigenin (1) (IC50 8.9) and ferulic acid (2) exhibited more ABTS•+ activity than standard Trolox (IC50 13.8 μg/mL). Vitexin (3) revealed the most antiproliferative activity against HeLa, HT29, C6 and Vero cells lines with IC50 values of 35.6, 32.5, 41.6, 46.7 (μM), respectively. Discussion and conclusion: Globoidnan A (6) has the most antioxidant effects on all assays. This has to do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent.en
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tübitak )
dc.identifier10.1016/s0731-7085(00)00514-8
dc.identifier.citationERENLER R., MERAL B., SEN O., ELMASTAS M., AYDIN A., EMINAGAOGLU O., Topcu G., -Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities.-, Pharmaceutical biology, cilt.55, ss.1646-1653, 2017
dc.identifier.doi10.1080/13880209.2017.1310906
dc.identifier.pubmed28431483
dc.identifier.scopus85032448944
dc.identifier.urihttps://hdl.handle.net/20.500.12645/2677
dc.identifier.wosWOS:000419990000001
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleBioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities.
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
local.avesis.id41803882-2fc4-48d9-b1cc-7e8f7d13bb4b
local.avesis.response2547
local.publication.isinternational1
relation.isAuthorOfPublication3c94748e-c303-4294-ad67-c5a0739628e5
relation.isAuthorOfPublication.latestForDiscovery3c94748e-c303-4294-ad67-c5a0739628e5
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
103- Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Yayınlar - Eserler
Eczacılık Fakültesi