Publication:
Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines

Simple item page
dc.contributor.authorGAZİOĞLU, IŞIL
dc.contributor.authorSemen, Sevcan
dc.contributor.authorAcar, Ozden Ozgun
dc.contributor.authorKOLAK, Ufuk
dc.contributor.authorSen, Alaattin
dc.contributor.authorTOPÇU, GÜLAÇTI
dc.contributor.institutionauthorGAZİOĞLU, IŞIL
dc.contributor.institutionauthorTOPÇU, GÜLAÇTI
dc.date.accessioned2020-09-28T20:59:10Z
dc.date.available2020-09-28T20:59:10Z
dc.date.issued2020-01-01T00:00:00Z
dc.description.abstractContext: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tubitak )
dc.identifier.citationGAZİOĞLU I., Semen S., Acar O. O. , KOLAK U., Sen A., TOPÇU G., -Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines-, PHARMACEUTICAL BIOLOGY, cilt.58, ss.925-931, 2020
dc.identifier.doi10.1080/13880209.2020.1814356
dc.identifier.pubmed32915696
dc.identifier.scopus85090857592
dc.identifier.urihttp://hdl.handle.net/20.500.12645/19644
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/13880209.2020.1814356
dc.identifier.wosWOS:000568886900001
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject3β-pentacosanoate
dc.subjectCapparaceae
dc.subjectAnti-inflammatory
dc.subjectAnticholinesterase
dc.subjectFatty acid
dc.subjectOlean-12-en-3β, 28-diol
dc.subjectSecondary metabolites
dc.subjectSpectroscopy
dc.subjectStructure elucidation
dc.titleTriterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines
dc.typeArticle
dspace.entity.typePublication
local.avesis.idd388df2a-e170-46ea-bedb-2a798fe0005b
local.publication.isinternational1
relation.isAuthorOfPublication336380d2-70ee-496d-a002-08059f9da500
relation.isAuthorOfPublication3c94748e-c303-4294-ad67-c5a0739628e5
relation.isAuthorOfPublication.latestForDiscovery3c94748e-c303-4294-ad67-c5a0739628e5
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines.pdf
Size:
1.26 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Yayınlar - Eserler