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Fabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk

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dc.contributor.authorTIRIS G.
dc.contributor.authorGazioglu I.
dc.contributor.authorFurton K. G.
dc.contributor.authorKabir A.
dc.contributor.authorLocatelli M.
dc.contributor.institutionauthorTIRIS, GİZEM
dc.contributor.institutionauthorGAZİOĞLU, IŞIL
dc.date.accessioned2023-01-08T22:54:02Z
dc.date.available2023-01-08T22:54:02Z
dc.date.issued2023-01-01
dc.description.abstractA fast procedure obtained by the combination of fabric phase extraction (FPSE) with high performance liquid chromatography (HPLC) has been developed and validated for the quantification of favipiravir (FVP) in human plasma and breast milk. A sol-gel polycaprolactone-block-polydimethylsiloxane-block-polycaprolactone (sol-gel PCAP-PDMS-PCAP) coated on 100% cellose cotton fabric was selected as the most efficient membrane for FPSE in human plasma and breast milk samples. HPLC-UV analysis were performed using a RP C18 column under isocratic conditions. Under these optimezed settings, the overall chromatographic analysis time was limited to only 5 min without encountering any observable matrix interferences. Following the method validation pro-cedure, the herein assay shows a linear calibration curve over the range of 0.2-50 mu g/mL and 0.5-25 mu g/mL for plasma and breast milk, respectively. The method sensitivities in terms of limit of detection (LOD) and limit of quantification (LOQ), validated in both the matrices, have been found to be 0.06 and 0.2 mu g/mL for plasma and 0.15 and 0.5 mu g/mL for milk, respectively. Intraday and interday precision and trueness, accordingly to the International Guidelines, were validated and were below 3.61% for both the matrices. The herein method was further tested on real samples in order to highlight the applicability and the advantage for therapeutic drug monitoring (TDM) applications. To the best of our knowledge, this is the first validated FPSE-HPLC-UV method in human plasma and breast milk for TDM purposes applied on real samples. The validated method provides fast, simple, cost reduced, and sensitive assay for the direct quantification of favipiravir in real biological matrices, also appliyng a well-known rugged and cheap instrument configuration.
dc.identifier.citationTIRIS G., Gazioglu I., Furton K. G., Kabir A., Locatelli M., "Fabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.223, 2023
dc.identifier.doi10.1016/j.jpba.2022.115131
dc.identifier.issn0731-7085
dc.identifier.pubmed36395627
dc.identifier.urihttps://hdl.handle.net/20.500.12645/34864
dc.identifier.volume223
dc.relation.ispartofJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEczacılık
dc.subjectTemel Eczacılık Bilimleri
dc.subjectYaşam Bilimleri
dc.subjectKimya
dc.subjectAnalitik Kimya
dc.subjectSağlık Bilimleri
dc.subjectTemel Bilimler
dc.subjectPharmacology and Therapeutics
dc.subjectBasic Pharmaceutics Sciences
dc.subjectLife Sciences
dc.subjectChemistry
dc.subjectAnalytical Chemistry
dc.subjectHealth Sciences
dc.subjectNatural Sciences
dc.subjectKİMYA, ANALİTİK
dc.subjectTemel Bilimler (SCI)
dc.subjectFARMAKOLOJİ VE ECZACILIK
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectCHEMISTRY, ANALYTICAL
dc.subjectCHEMISTRY
dc.subjectNatural Sciences (SCI)
dc.subjectPHARMACOLOGY & PHARMACY
dc.subjectPHARMACOLOGY & TOXICOLOGY
dc.subjectLife Sciences (LIFE)
dc.subjectFarmakoloji
dc.subjectFarmakoloji, Toksikoloji ve Eczacılık (çeşitli)
dc.subjectGenel Farmakoloji, Toksikoloji ve Eczacılık
dc.subjectFarmakoloji (tıbbi)
dc.subjectİlaç Rehberleri
dc.subjectKimya (çeşitli)
dc.subjectGenel Kimya
dc.subjectFiltrasyon ve Ayırma
dc.subjectFizik Bilimleri
dc.subjectPharmacy
dc.subjectPharmacology
dc.subjectPharmacology, Toxicology and Pharmaceutics (miscellaneous)
dc.subjectGeneral Pharmacology, Toxicology and Pharmaceutics
dc.subjectPharmacology (medical)
dc.subjectDrug Guides
dc.subjectChemistry (miscellaneous)
dc.subjectGeneral Chemistry
dc.subjectFiltration and Separation
dc.subjectPhysical Sciences
dc.subjectHPLC
dc.subjectFavipiravir
dc.subjectFabric phase sorptive extraction
dc.subjectPlasma
dc.subjectBreast milk
dc.subjectTDM
dc.titleFabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk
dc.typearticle
dspace.entity.typePublication
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relation.isAuthorOfPublication1ab47475-d54a-4b46-a679-47fb775bc1ce
relation.isAuthorOfPublication336380d2-70ee-496d-a002-08059f9da500
relation.isAuthorOfPublication.latestForDiscovery1ab47475-d54a-4b46-a679-47fb775bc1ce
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