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1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro

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dc.contributor.authorMUCUK, GÖKŞEN
dc.contributor.authorSEPET, ELİF
dc.contributor.authorERGÜVEN, MİNE
dc.contributor.authorEKMEKÇİ, ÖZLEM
dc.contributor.authorBİLİR, AYHAN
dc.contributor.institutionauthorMUCUK, GÖKŞEN
dc.date.accessioned2020-10-29T14:09:21Z
dc.date.available2020-10-29T14:09:21Z
dc.date.issued2017-01-01T00:00:00Z
dc.description.abstractBackground: 1,25-Dihydroxyvitamin D-3 (1,25-OH D-3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). Aim: This study aimed to understand the influence of various concentrations of 1,25-OH D-3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. Materials and Methods: hDPSCs were obtained from the impacted third molar teeth. Monolayer cultured cells were incubated with a differentiation medium containing different concentrations of 1,25-OH D-3 (0.001, 0.01, and 0.1 mu M). All groups were evaluated by S-phase rate [immunohistochemical (IHC) bromodeoxyuridine (BrdU) staining], STRO-1 and dentin sialoprotein (DSP)+ levels (IHC), and alkaline phosphatase (ALP, enzyme-linked immunosorbent assay (ELISA)) levels. Results: The number of cells that entered the S-phase was determined to be the highest and lowest in the control and 0.001 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M vitamin D-3 group had the highest increase in DSP+ levels. The highest Stro-1 levels were detected in the control and 0.1 mu M 1,25-OH D-3 groups, respectively. The 0.1 mu M 1,25-OH D-3 induced a mild increase in ALP activity. Conclusions: This study demonstrated that 1,25-OH D-3 stimulated odontoblastic differentiation of hDPSCs in vitro in a dose-dependent manner. The high DSP + cell number and a mild increase in ALP activity suggest that DPSCs treated with 0.1 M 1,25-OH D-3 are in the later stage of odontoblastic differentiation. The results confirm that 1,25-OH D-3-added cocktail medium provides a sufficient microenvironment for the odontoblastic differentiation of hDPSCs in vitro.
dc.identifier.citationMUCUK G., SEPET E., ERGÜVEN M., EKMEKÇİ Ö., BİLİR A., -1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro-, CONNECTIVE TISSUE RESEARCH, cilt.58, no.6, ss.531-541, 2017
dc.identifier.doi10.1080/03008207.2016.1264395
dc.identifier.scopus85009999430
dc.identifier.urihttp://hdl.handle.net/20.500.12645/25269
dc.identifier.wosWOS:000415941900005
dc.title1,25-Dihydroxyvitamin D-3 stimulates odontoblastic differentiation of human dental pulp-stem cells in vitro
dc.typeArticle
dspace.entity.typePublication
local.avesis.id1e446ca5-6eff-4124-869a-18dbff43256e
local.publication.isinternational1
relation.isAuthorOfPublication41f24a42-557b-4e9d-9bde-e9f615b54630
relation.isAuthorOfPublication.latestForDiscovery41f24a42-557b-4e9d-9bde-e9f615b54630
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