Person: ÜSTÜNOVA, SAVAŞ
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Publication Metadata only Role of ischemic preconditioning and tempol in ischemia/reperfusion injury in isolated rat heart(2013-07-01T00:00:00Z) Erol, D.; Ustunova, SAVAŞ; Gurel, E.; Dedeakayogullari, H.; Demirci-Tansel, C.; ÜSTÜNOVA, SAVAŞPublication Metadata only Hexokinase cellular trafficking in ischemia-reperfusion and ischemic preconditioning is altered in type I diabetic heart(2013-07-01T00:00:00Z) Gurel, Ebru; Ustunova, SAVAŞ; Kapucu, Aysegul; Yilmazer, Nadim; EERBEEK, Otto; NEDERLOF, Rianne; HOLLMANN, Markus W.; Demirci-Tansel, Cihan; ZUURBIER, Coert J.; ÜSTÜNOVA, SAVAŞDiabetes mellitus (DM) has been reported to alter the cardiac response to ischemia-reperfusion (IR). In addition, cardioprotection induced by ischemic preconditioning (IPC) is often impaired in diabetes. We have previously shown that the subcellular localisation of the glycolytic enzyme hexokinase (HK) is causally related to IR injury and IPC protective potential. Especially the binding of HK to mitochondria and prevention of HK solubilisation (HK detachment from mitochondria) during ischemia confers cardioprotection. It is unknown whether diabetes affects HK localisation during IR and IPC as compared to non-diabetes. In this study we hypothesize that DM alters cellular trafficking of hexokinase in response to IR and IPC, possibly explaining the altered response to IR and IPC in diabetic heart. Control (CON) and type I diabetic (DM) rat hearts (65 mg/kg streptozotocin, 4 weeks) were isolated and perfused in Langendorff-mode and subjected to 35 min I and 30 min R with or without IPC (3 times 5 min I). Cytosolic and mitochondrial fractions were obtained at (1) baseline, i.e. after IPC but before I, (2) 35 min I, (3) 5 min R and (4) 30 min R. DM improved rate-pressure product recovery (RPP; 71 +/- A 10 % baseline (DM) versus 9 +/- A 1 % baseline (CON) and decreased contracture (end-diastolic pressure: 24 +/- A 8 mmHg (DM) vs 77 +/- A 4 mmHg (CON)) after IR as compared to control, and was associated with prevention of HK solubilisation at 35 min I. IPC improved cardiac function in CON but not in DM hearts. IPC in CON prevented HK solubilisation at 35 min I and at 5 min R, with a trend for increased mitochondrial HK. In contrast, the non-effective IPC in DM was associated with solubilisation of HK and decreased mitochondrial HK at early reperfusion and a reciprocal behaviour at late reperfusion. We conclude that type I DM significantly altered cellular HK translocation patterns in the heart in response to IR and IPC, possibly explaining altered response to IR and IPC in diabetes.Publication Metadata only Herbal Haemorrhoidal Cream for Haemorrhoids(2013-10-31T00:00:00Z) Gurel, Ebru; Ustunova, SAVAŞ; Ergin, Bulent; Tan, Nur; Caner, Metin; Tortum, Osman; Demirci-Tansel, Cihan; ÜSTÜNOVA, SAVAŞAlthough hemorrhoids are one of the most common diseases in the world, the exact etiology underlying the development of hemorrhoids is not clear. Many different ointments are currently used to treat hemorrhoids; however, there is little evidence of the efficacy of these treatments to support their use. The aim of this study was to compare different herbal creams used for the treatment of hemorrhoids. Twenty-eight male Wistar albino rats, 6-8 weeks old and weighing 160-180 g, were used in this study as 1-control, 2-croton oil, 3-croton oil+fig leaves+artichoke leaves+walnut husks and 4-croton oil+fig leaves+artichoke leaves+walnut husks+horse chestnut fruit. After 3 days of croton oil application, rats were treated with 0.1 ml of cream or saline twice a day for 15 days by syringe. Tissue and blood samples were collected for histological, immunohistochemical and biochemical studies. Statistical significance was determined using one-way ANOVA followed by Tukey-s multiple comparison tests. Croton oil administration resulted in severe inflammation. The third group showed partial improvement in inflammation; however, the greatest degree of improvement was seen in the fourth group, and some recovered areas were observed. Myeloperoxidase immunoreactivity was found to be decreased in the third and fourth groups compared to the second group. Additionally, biochemical analyses (Myeloperoxidase, Malondyaldehyde, nitrate/nitrite and nitrotyrosine levels and Superoxide Dismutase activity) were in agreement with the histological and immunohistochemical results. In conclusion, croton oil causes inflammation in the anal area and results in hemorrhoids. Treatment with our herbal hemorrhoid creams demonstrated anti-inflammatory and anti-oxidant effects in this model.Publication Metadata only Staphylococcus aureus-induced sepsis and Coenzyme Q10 therapy: an isolated rat heart study(2013-07-01T00:00:00Z) Ustunova, SAVAŞ; Gurun, S.; Gurel, E.; Dedeakayogullari, H.; Demirci-Tansel, C.; ÜSTÜNOVA, SAVAŞPublication Metadata only The Effects of Cichorium intybus Extract on Cerebellar Apoptosis and Oxidative Stress in Pentylenetetrazole-induced Kindling Model in Rats(2016-09-01) Meral, Ismail; Gurevin, Ebru Gurel; Ustunova, SAVAŞ; DEMIRTAS, Murat; Ozturk, Nilgun; Kiyan, Hulya Tuba; Armutak, Elif Ilkay; Sogut, Ibrahim; Erkeç, Özlem Ergül; MERAL, İSMAİL; ÜSTÜNOVA, SAVAŞPublication Metadata only Hexokinase cellular trafficking in response to ischemic preconditioning and ischemia-reperfusion is altered in the diabetic heart(2011-06-01T00:00:00Z) Gurel, E.; Ustunova, SAVAŞ; Kapucu, A.; Yilmazer, N.; EERBEEK, O.; NEDERLOF, R.; Demirci-Tansel, C.; ZUURBIER, C. J.; ÜSTÜNOVA, SAVAŞPublication Metadata only Herbal haemorrhoidal cream for the treatment of haemorrhoids induced by croton oil in rats(2011-06-01T00:00:00Z) Ustunova, SAVAŞ; Ergin, B.; Gurel, E.; Caner, M.; Tortum, O.; Demirci-Tansel, C.; ÜSTÜNOVA, SAVAŞPublication Open Access Possible effects of Phillyrea latifolia on weight loss in rats fed a high-energy diet(2016-01-01) Yazici-Tutunis, Secil; Gurel-Gurevin, Ebru; Ustunova, SAVAŞ; Demirci-Tansel, Cihan; Mericli, Filiz; ÜSTÜNOVA, SAVAŞContext Phillyrea latifolia L. (Oleaceae), commonly found in the Mediterranean region in Turkey, is used as medicinal teas for weight loss and hyperglycaemia in folk medicine.Publication Metadata only Angiotensin II type 2 receptor blocker PD123319 has more beneficial effects than losartan on ischemia/reperfusion injury and oxidative damage in isolated rat heart.(2019-07-30) Kilic, AYSU; Ustunova, SAVAŞ; Usta, C; Bulut, H; Demirci, Tansel; Gurel, Gurevin; KILIÇ, AYSU; ÜSTÜNOVA, SAVAŞ; MERAL, İSMAİLPublication Metadata only Estrogen receptor agonists alleviate cardiac and renal oxidative injury in rats with renovascular hypertension(2016-01-01) Kumral, Zarife Nigar Ozdemir; Kolgazi, Meltem; Ustunova, SAVAŞ; Cakir, Ozguer Kasimay; Cevik, Ozge Dagdeviren; ŞENER, GÖKSEL; YEGEN, BERRAK; ÜSTÜNOVA, SAVAŞAlthough endogenous estrogen is known to offer cardiac and vascular protection, the involvement of estrogen receptors in mediating the protective effect of estrogen on hypertension-induced cardiovascular and renal injury is not fully explained. We aimed to investigate the effects of estrogen receptor (ER) agonists on oxidative injury, cardiovascular and renal functions of rats with renovascular hypertension (RVH). Female Sprague-Dawley rats were randomly divided as control and RVH groups, and RVH groups had either ovariectomy (OVX) or sham-OVX. Sham-OVX-RVH and OVX-RVH groups received either ER agonist diarylpropiolnitrile (1 mg/kg/day) or ER agonist propyl pyrazole triol (1 mg/kg/day) for 6 weeks starting at the third week following the surgery. At the end of the 9(th) week, systolic blood pressures were recorded, cardiac functions were determined, and the contraction/relaxation responses of aortic rings were obtained. Serum creatinine levels, tissue malondialdehyde, glutathione, superoxide dismutase, catalase levels, and myeloperoxidase activity in heart and kidney samples were analyzed, and Na+, K+-ATPase activity was measured in kidney samples. In both sham-OVX and OVX rats, both agonists reduced blood pressure and reversed the impaired contractile performance of the heart, while ER agonist improved renal functions in both the OVX and non-OVX rats. Both agonists reduced neutrophil infiltration, lipid peroxidation, and elevated antioxidant levels in the heart, but a more ER-mediated protective effect was observed in the kidney. Our data suggest that activation of ER might play a role in preserving the function of the stenotic kidney and delaying the progression of renal injury, while both receptors mediate similar cardioprotective effects.