Person: KÜTÜK, NÜKHET
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Publication Metadata only Effects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells(2019-10-13) Salkin, Hasan; GÖNEN, ZEYNEP BURÇİN; ÖZCAN, SERVET; Lekesizcan, Ayca; BAHAR, DİLEK; KÜTÜK, NÜKHET; ALKAN, ALPER; KÜTÜK, NÜKHET; ALKAN, ALPERAim: The aim of this study was to evaluate the effects of standard culture medium and chondrogenic differentiation medium with PRP on chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells (rabbit DPSCs) that are transfected with transforming growth factor-beta 1 (TGF-B1) gene, based on the hypothesis of TGF- B1 and PRP can be effective on the chondrogenesis of stem cells. Materials and Methods: Rabbit DPSCs were characterized by using flow cytometry, immunofluorescent staining, quantitative Real Time Polymerase Chain Reaction (qRT-PCR) and differentiation tests. For the characterization, CD29, CD44 and CD45 mesenchymal cell markers were used. Rabbit DPSCs were transfected with TGF-B1 gene using electroporation technique in group 1; with PRP 10% in group 2; with chondrogenic medium in group 3; with both chondrogenic medium and PRP in group 4. DPSCs were cultured in medium with 10% inactive PRP in group 5, chondrogenic medium in group 6, chondrogenic medium with PRP 10% in group 7. SOX9, MMP13 and Aggrecan gene expression levels were evaluated in 3, 6, 12. and 24. days by qRT-PCR. Results: The expression levels of SOX9, MMP13 and Aggrecan were higher in group 2, 3 and group 7 in 3th day however in 24th day group 7 and group 2 were found higher. The expression levels changed by time-dependent. The extracellular matrix of the cells in experimental groups were positively stained with safranin O and toluidine blue. Conclusion: The combination in culture medium of TGF-B1 gene transfection and 10% PRP accelerates the chondrogenic differentiation of DPSCs.Publication Metadata only Vertical ridge augmentation using guided bone regeneration procedure and dental pulp derived mesenchymal stem cells with simultaneous dental implant placement: A histologic study in a sheep model(2019-06-01) Colpak, H. A.; GÖNEN, ZEYNEP BURÇİN; Ozdamar, S.; ALKAN, ALPER; KÜTÜK, NÜKHET; ALKAN, ALPER; KÜTÜK, NÜKHETObjectives: To determine the effects of bone graft and dental pulp derived mesenchymal stem cells (DPMSCs) implantation with simultaneous dental implant placement on osteointegration, newly formed bone and vertical bone height histologically and histomorphometrically in a sheep model.Publication Metadata only Evaluation of Vestibular Bone Thickness in Class I Malocclusion Treatment With Corticotomy-Assisted Rapid Orthodontics(2019-11-01T00:00:00Z) GÖNEN, ZEYNEP BURÇİN; ALKAN, Alper; Ekizer, Abdullah; KÜTÜK, Nükhet; Tasdemir, Zekeriya; ALKAN, ALPER; KÜTÜK, NÜKHETBackground: Corticotomy-assisted rapid orthodontics is a widely used method for speeding up conventional orthodontics. This study (i) evaluates the effects of corticotomy alone, corticotomy combined with bone graft, and corticotomy with platelet-rich fibrin (PRF) on vestibular alveolar bone thickness in patients with class I malocclusion; (ii) compares the treatment time with a conventional orthodontic therapy group, and (iii) investigates the periodontal health of patients who have undergone corticotomy-assisted rapid orthodontics.Publication Metadata only Kortikotomi Destekli Hızlı Ortodontik Tedavi Gören Sınıf I Çapraşıklığa Sahip Hastalarda Vestibul Kemik Kalınlığının Değerlendirilmesi(2018-12-08T00:00:00Z) GÖNEN, ZEYNEP BURÇİN; ALKAN, ALPER; EKİZER, ABDULLAH; KÜTÜK, NÜKHET; TAŞDEMİR, ZEKERİYA; ALKAN, ALPER; KÜTÜK, NÜKHETPublication Metadata only The Optimal Isolation and Characterization Conditions ofRabbit Dental Pulp-Derived Mesenchymal Stem Cells for Use inRegenerative Medicine(2019-06-01T00:00:00Z) SALKIN, HASAN; GÖNEN, ZEYNEP BURÇİN; ÖZCAN, SERVET; BAHAR, DİLEK; KÜTÜK, NÜKHET; ALKAN, ALPER; KÜTÜK, NÜKHET; ALKAN, ALPERPublication Metadata only Reliability of Panoramic Radiography in Determination of Neurosensory Disturbances Related to Dental Implant Placement in Posterior Mandible(2014-12-01T00:00:00Z) KÜTÜK, Nükhet; GÖNEN, ZEYNEP BURÇİN; Yasar, M. Taha; DEMİRBAŞ, Ahmet Emin; ALKAN, Alper; KÜTÜK, NÜKHET; ALKAN, ALPERIntroduction: During implantology procedures, one of the most serious complications is the damage of the inferior alveolar nerve, which may result in neurosensory disturbances (NSD). Panoramic radiographs have been considered for a primary evaluation to determine the bone height and implant-mandibular canal distance.Publication Metadata only Analysis of Traumatic Bone Cyst of the Jaws: A Retrospective Study(2019-03-01T00:00:00Z) DEMİRBAŞ, AHMET EMİN; ÇOLPAK, HALİS ALİ; KÜTÜK, NÜKHET; GÖNEN, ZEYNEP BURÇİN; ALKAN, ALPER; KÜTÜK, NÜKHET; ALKAN, ALPER