Person: TEKKELİ, ŞERİFE EVRİM
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Publication Open Access An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization(2017-03-01) Tekkeli, S. E. Kepekci; GAZIOGLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞILA new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.Publication Metadata only SIMULTANEOUS DETERMINATION OF FEBUXOSTAT AND MONTELUKAST IN HUMAN PLASMA BY USING FABRIC PHASE SORPTIVE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(2021-09-17T00:00:00Z) GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM; Kabir, Abuzar; Aslan, Ceylin; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİMPublication Metadata only High performance liquid chromatographic analysis of lercanidipine in human breast milk(2019-01-01) Tekkeli, Evrim Kepekci; GAZİOĞLU, IŞIL; TIRIS, GİZEM; Onal, Cem; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; TIRIS, GİZEMA simple, rapid, precise and accurate isocratic reversed phase HPLC method was developed and validated for the determination of lercanidipine hydrochloride in pharmaceutical tablets and spiked human breast milk. The chromatographic separation was achieved on C18 (250x4.6 mmx5 mu m) column using a mobile phase consisting of acetonitrile and phospate buffer (pH=4) (55:45, v/v) at a flow rate of 1.1 mL/min and UV detection at 237 nm. The linearity of the proposed method was investigated in the range of 1.0-40 mu g/mL (r(2)=0.9990). The method was validated in terms of accuracy, precision, reproducibility, specificity, robustness, and detection and quantification limits, in accordance with ICH guidelines. The proposed method is found as suitable for routine quantification of lercanidipine in human breast milk.Publication Metadata only HPLC Method for Moxifloxacin in Breast Milk By Fluorimetric Detection with Precolumn Derivatization(2015-10-17) TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞILPublication Open Access Development and validation of a HPLC method for the determination of benzo(a)pyrene in human breast milk(2017-04-01) Tekkeli, Serife Evrim Kepekci; Gazioglu, IŞIL; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİMA simple analytical procedure was developed for the quantitation of benzo(a)pyrene in human breast milk using solid phase extraction (SPE) combined with high performance liquid chromatography. Before the chromatographic process, SPE, including C18 functional groups in silicagel cartridges, was conducted for sample preparation. A C18 column (100×4.6 mm id, 3 μm particle size) was used with acetonitrile:water (80:20) as the mobile phase at a flow rate 1mL/min at 30°C. Fluorimetric detection was performed for excitation and emission at 290 and 406 nm, respectively. It was observed that the calibration curve was linear over the range of 0.5-80 ng/mL. The limit of detection and limit of quantitation were found to be 0.5 and 1.07 ng/mL, respectively. Intraday and interday relative standard deviation values were less than 5.15%. Moreover, the newly developed method provides a fast, simple, cost effective, and sensitive assay to detect an important carcinogen substance, benzo(a)pyrene, in human breast milk.Publication Metadata only Development of sol-gel phenyl/methyl/poly (dimethylsiloxane) sorbent coating for fabric phase sorptive extraction and its application in monitoring human exposure to selected polycyclic aromatic hydrocarbons using high performance liquid chromatography-fluorescence detection(2021-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Kabir, Abuzar; Zengin, Ozge S.; Tekkeli, Evrim Kepekci; Furton, Kenneth G.; Tartaglia, Angela; Locatelli, Marcello; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİMFollowing the convenient, yet very powerful pathway to create designer extraction sorbent using sol-gel chemistry, a novel sol-gel phenyl/methyl/poly(dimethylsiloxane) sorbent coating was created on polyester fabric substrate for fabric phase sorptive extraction (FPSE) and was subsequently applied to monitor human exposure to selected polycyclic aromatic hydrocarbons (PAHs) including pyrene, chrysene, and benzo[a]pyrene in plasma samples obtained from tobacco smoker volunteers using high performance liquid chromatography fluorescence detector (HPLC-FLD). A rapid FPSE-HPLC-FLD method was developed that adequately resolved the PAHs chromatographically, after their successful extraction from human plasma using fabric phase absorption extraction (FPSE) and subsequently analysed in the liquid chromatographic system by means of an analytical column (InterSustain C-18 column 150 x 4.6 mm, 5 mu m) using acetonitrile (ACN) and water as mobile phases in gradient elution mode. With the optimized conditions, the retention times were found to be 6.168, 7.214, and 10.404 min for pyrene, chrysene, and benzo[a]pyrene, respectively. The total chromatographic runtime was limited to 12.5 min.Publication Metadata only Simultaneous determination of febuxostat and montelukast in human plasma using fabric phase sorptive extraction and high performance liquid chromatography-fluorimetric detection.(2021-12-05T00:00:00Z) Evrim Kepekci Tekkeli, S; Tartaglia, Angela; Aslan, Ceylin; Locatelli, Marcello; Kabir, Abuzar; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM