Person: GÜLER, ERAY METİN
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Publication Metadata only Cytotoxic, genotoxic and apoptotic activities of carvacrol from Thymus vulgaris on the human adenocarcinoma gastric cells(2015-08-01) GUNES-BAYIR, Ayse; Kiziltan, HURİYE ŞENAY; Guler, ERAY METİN; KARAKAS, Ersin; Kocyigit, ABDÜRRAHİM; GÜNEŞ BAYIR, AYŞE; KIZILTAN, HURİYE ŞENAY; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Assessment of the effectiveness of cyclosporine nasal spray in an animal model of allergic rhinitis(2018-01-01) Senturk, EROL; Yildirim, YAVUZ SELİM; Dogan, REMZİ; Ozturan, ORHAN; Guler, ERAY METİN; AYDIN, Mehmet Serif; Kocyigit, ABDÜRRAHİM; Esrefoglu, MUKADDES; Kocak, Ilker; ŞENTÜRK, EROL; YILDIRIM, YAVUZ SELİM; DOĞAN, REMZI; ÖZTURAN, ORHAN; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDESThe aim of this study is to show if cyclosporine has an antiallergic role in a rat model of ovalbumin-induced allergic rhinitis. The 54 rats were divided into six equal groups. The first group was a negative control group without induced allergic rhinitis; the second group a positive control with induced allergic rhinitis not receiving treatment. The remaining four groups, after induction of allergic rhinitis, received intranasal cyclosporine treatment in doses of 0.05, 0.1, or 0.2% or nasal steroid treatment. In the biochemical examination, on the surface of the tissue tumor necrosis factor (TNF) interferon (IFN), interleukin (IL)-5, IL-13, as well as IL-2, IL-4, IL-17A, and IgE were studied. Histologically, ciliary loss, increase of goblet cells, vascular congestion, and the degree of eosinophil infiltration were rated. In all treatment groups, on average, a significant reduction in all histological and biochemical values was found compared to the positive control group. Comparing each of the three cyclosporine-using groups with the group of nasal corticosteroid did not show any significant difference in the average scores. Cyclosporine nasal drops are effective to be used in an animal model of experimental allergic rhinitis without systemic effects.Publication Metadata only Curcumin induce DNA damage and apoptosis through generation of reactive oxygen species and reducing mitochondrial membrane potential in melanoma cancer cells(2017-01-01) Kocyigit, ABDÜRRAHİM; Guler, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİNMelanoma is the most malignant skin cancer. Curcumin has shown to have therapeutic effects when used in the treatment of malignant diseases. However, the precise molecular mechanisms of its action are not fully elucidated. In this research, we hypothesized that reactive oxygen species (ROS) play a key role in curcumin induced DNA damage, apoptosis and cell dead. To test our hypothesis, cytotoxic, genotoxic, apoptotic, ROS generating and mitochondrial membrane potential (MMP) of curcumin on mouse melanoma cancer cells (B16-F10) and fibroblastic normal cells (L-929) were investigated. Our results demonstrated that curcumin decreased cell viability and MMP and, increased DNA damage, apoptosis and ROS levels in both melanoma cancer and normal cells in a dose dependent manner and, these activities were significantly higher in melanoma cells than in normal cells with higher concentrations. There were positive strong relationships between DNA damage, apoptosis, cytotoxicity and ROS generation and MMP levels in curcumin treated melanoma and normal cells. In summary, this in vitro study provide clear evidence that curcumin induced DNA damage, apoptosis and cytotoxicity via its pro-oxidant activity in a dose dependent manner in both cancer and normal cells and these activities were higher in cancer cells than those of normal cells.Publication Metadata only Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.(2018-11-01) Günes-Bayir, AYŞE; Kocyigit, ABDÜRRAHİM; Güler, ERAY METİN; Bilgin, MEHMET GÜLTEKİN; Ergün, İLYAS SAMET; DADAK, A; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; BİLGİN, MEHMET GÜLTEKİN; ERGÜN, İLYAS SAMETPublication Metadata only Thymoquinone Against Gastric Cancer: A New Hope of Therapy(2017-10-01) TURKDOGAN, M. Kursad; Kocyigit, ABDÜRRAHİM; Guler, ERAY METİN; OZER, O. Faruk; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; ÖZER, ÖMER FARUKPublication Metadata only Synthesis of Oleanolic Acid Analogues and Their Cytotoxic Effects on 3T3 Cell Line(2018-01-01) Tuncay, Salih; Kocyigit, ABDÜRRAHİM; SEÇEN, Hasan; Ocal, Nuket; Topcu, GÜLAÇTI; Guler, ERAY METİN; ŞENOL, HALIL; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; TOPÇU, GÜLAÇTIBackground: Oleanolic acid (OA) is a known natural compound with many important biological activities. Thirteen oleanolic acid derivatives linked at C-3 and C-28 were synthesized and their structures were confirmed by H-1- and C-13 NMR and mass spectral analyses. Among them, compounds 4, 6, 8-10, 12, 13 were synthesized for the first time. They were evaluated for their cytotoxic activity. They showed proliferative effect at low concentrations while cytotoxic effect was observed at high concentrations in a dose dependent manner.Publication Metadata only Curcumin induced cytotoxicity by oxidative stress mediated DNA damage and apoptosis in mouse B16F10 melanoma cells(2015-08-01T00:00:00Z) Kocyigit, ABDÜRRAHİM; Guler, Eray M.; KARATAS, Ersin; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİNPublication Metadata only Ankaferd hemostat induces DNA damage, apoptosis and cytotoxic activity by generating reactive oxygen species in melanoma and normal cell lines(2017-01-01) Kocyigit, ABDÜRRAHİM; Guler, ERAY METİN; HAZNEDAROĞLU, İBRAHİM CELALETTİN; Malkan, Umit Yavuz; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİNAlthough, Ankaferd hemostat (Ankaferd Blood Stopper, ABS) could be utilized successfully as hemostatic agent, studies demonstrated that it has cytotoxic effects on cells. However, the mechanism(s) of this effect has not been elucidated yet. In this study, cytotoxic, genotoxic, apoptotic and reactive oxygen generating (ROS) activities of ABS were investigated in melanoma and normal cell lines. The cells were incubated with different concentrations of ABS (0.125 to 2%) for 24 h. The cell viability was assessed based on ATP cell viability assay. Intracellular accumulation of reactive oxygen species (ROS) was determined using the fluorescent probes 2-,7--dichloro-dihydrofluoresce-in-diacetate (H2DCF-DA). DNA damage was evaluated by alkaline single cell gel electrophoresis assay (Comet Assay) and, apoptosis induction was detected by Acridine Orange/Ethidium Bromide AO/EB double staining method. Our results demonstrated that ABS increases DNA damage, apoptosis and ROS levels in both melanoma and normal cell lines in a dose dependent manner, and all of these activities were significantly higher in melanoma cells than in normal cells. There was a statistically significant positive correlation between DNA damage, apoptosis and ROS levels in ABS treated cell lines. Our results revealed that although ABS commonly used as hemostatic agent, it causes DNA damage and apoptosis by generating ROS in a dose dependent manner. Therefore, it should be removed the unused ABS by cleaning once the hemostasis is achieved to minimize the postoperative side effects. These results could also contribute to the development of new treatment for cancer.Publication Metadata only Antioxidant and anticancer effect of Artemisia absinthium extract on colon and endometrium adenocarcinoma cells(2016-09-01) Koyuncu, I.; Kirmit, A.; GULER, ERAY METİN; Yuksekdag, O.; Kocyigit, ABDÜRRAHİM; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only The protective effect of newly derivatized compound naringenin-oxime and relative to naringenin against cisplatin-induced nephrotoxicity and genotoxicity in rat(2016-09-01) Koyuncu, I.; GULER, E. M.; Turkkan, B.; Yuksekdag, O.; Kocyigit, ABDÜRRAHİM; Kirmit, A.; Ozer, A.; SELEK, S.; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN