Person: GÖREN, AHMET CEYHAN
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Publication Metadata only Chemical composition and biological activities of Cypriot propolis(2021-09-01T00:00:00Z) NALBANTSOY, AYŞE; SARIKAHYA, NAZLI; Ozverel, Cenk Serhan; Barlas, Ayse Bercin; Kirci, Damla; AKGÜN, İSMAİL HAKKI; Yalcin, Tansel; Duven, Gamze; KIŞLA, DUYGU; DEMİRCİ, BETÜL; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHANPropolis compositions are highly variable, depending on the geographic region and the season of collection. In this study, propolis samples from seven different regions of Cyprus were studied for the first time by means of chemical content and biological activities. Secondary metabolite composition was determined by LC-HRMS. While the major flavonoids found were isosakuranetin, naringenin, rhamnocitrin, diosmetin, chrysin and acacetin, interestingly verbascoside, a phenylethanoid glycoside, and chlorogenic acid were identified as the major compounds in the ethanol-water extracts. alpha-Pinene was detected as the major compound of propolis extracts according to the volatile compositions via GC-MS. Karaoglanoglu and Tirmen extracts, presenting different chemical profiles, exerted enormous cytotoxic activity by MTT assay (IC50: 2.36-11.56 mu g/mL; 1.44-9.33 mu g/mL, respectively). The highest iNOS inhibition potential was detected in the Karpaz extract (IC50:2.6 mu g/mL) in LPS induced RAW 264.7 cells whereas the Guzelyurt sample demonstrated remarkable antioxidant (88.82 +/- 0.10%) and antimicrobial activities (with a MIC value of 31.2 mu g/mL against S. aureus, S. epidermidis, E. faecium, and E. faecalis).Publication Metadata only Diterpenoid and phenolic contents of Sideritis hololeuca Boiss & Heldr. Apud Bentham with antioxidant and anticholinesterase activity(2020-05-01T00:00:00Z) Carikci, Sema; GÖREN, AHMET CEYHAN; Kilic, Turgut; GÖREN, AHMET CEYHANPublication Metadata only Ent-kaurene Diterpenoids from Sideritis lycia with Antiviral and Cytotoxic Activities(2020-07-01T00:00:00Z) Kilic, Turgut; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; AYDOĞMUŞ, Zeynep; KARAGÖZ, Ali; Yildiz, Yasar K.; Aslan, Irfan; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHANThe genus Sideritis (Lamiaceae) is represented by 45 species (54 taxa) in Anatolia with high endemism ratio (74%), and Turkey is one of the gene centers of the genus along with Spain. Acetone extract of the aerial parts of Sideritis lycia afforded eight known ent-kaurene diterpenoids, structures of which have been identified as linearol, isolinearol, isosidol, sidol, siderol, sideridiol, 7-epi-candicandiol and foliol through 1H NMR, 13 C NMR and mass spectroscopic analyses. Cytotoxic and antiviral activities of the acetone extract, linearol, sidol and isosidol were investigated together with insecticidal activity of species. The antiviral index of linearol, isosidol and acetone extract of S. lycia were determined as 2.31, 2.01 and 2.58, respectively, except sidol. 7-Epi-candicandiol was found to be the most active diterpene against a series of cancer cell lines with ED50 values; KB (13.3 mu g/mL), COL-2 (11.8 mu g/mL), LU1 (17.9 mu g/mL), LNCaP (14.9 mu g/mL) and A2780 (9.0 mu g/mL). Activity results of this study indicated that ent-kaurane diterpenes have potential to be considered as antiviral and cytotoxic lead compounds.Publication Metadata only Synthesis of gamma-glutamyl beta-cyanoalanine precursor(2020-10-01T00:00:00Z) Un, Sule Sahin; Altundas, Ramazan; GÖREN, AHMET CEYHAN; GÖREN, AHMET CEYHANThe synthesis of precursors of neurotoxic gamma-glutamyl beta-cyanoalanine was developed starting from L-Serine via through the preparation of beta-cyanoalanine and glutamyl units. Coupling of these two intermediates gave methyl ester of gamma-glutamyl beta-cyanoalanine precursor. (C) 2020 ACG Publications. All rights reserved.Publication Metadata only Determination of secondary metabolites of Origanum vulgare subsp. hirtum and O. vulgare subsp. vulgare by LC-MS/MS(2020-01-01T00:00:00Z) Ozer, Zuleyha; Carikci, Sema; Yilmaz, Hasibe; Kilic, Turgut; Dirmenci, Tuncay; GÖREN, AHMET CEYHAN; GÖREN, AHMET CEYHANSecondary metabolites of solvent extracts of Origanum vulgare subsp. hirtum and O. vulgare subsp. vulgare were determined using liquid chromatography-mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Rosmarinic acid was determined as the main compound of studied extracts together with other phenolic acid derivatives. In LC-MS/MS analyses, relative standard deviations (RSD %) ranged between 0.11-9.47. The correlation values were found to be greater than 0.97 for each investigated analyte.Publication Metadata only Composition of the essential oil of Satureja metastasiantha: a new species for the flora of Turkey(2020-01-01T00:00:00Z) Carikci, Sema; GÖREN, AHMET CEYHAN; Dirmenci, Tuncay; Yalcinkaya, Burhanettin; Erkucuk, Aysen; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTIThe aerial parts of Satureja metastasiantha were hydrodistilled for 3 h using a Clevenger-type apparatus. The essential oils were analyzed by gas chromatography/flame ionization detector and gas chromatography/mass spectrometry, simultaneously, the main compounds of which were characterized as p-cymene (22.3%), thymol (21.0%), carvacrol (18.45%), and gamma-terpinene (12.1%). Antioxidant capacity, acetylcholinesterase and butyryl-cholinesterase inhibition effects, and antimicrobial and antifungal properties of the species were evaluated. The anticholinesterase activity of the essential oil of S. metastasiantha was observed with 30% inhibition at 200 mu g/mL. The essential oil of the species showed activity against Staphylococcus aureus with 128 mu g/mL minimum inhibitory concentration value.Publication Metadata only Identification of natural compounds of Jurinea species by LC-HRMS and GC-FID and their bioactivities(2021-08-01T00:00:00Z) SARIKAHYA, NAZLI; Okkali, Gaye Sumer; Celenk, Veysel U.; Mertoglu, Elif; Pekmez, Murat; Arda, Nazli; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHANThe Jurinea Cass. is one of the most important genera within Asteraceae and it comprises about 250 species in total. This genus is known for its numerous biological activities such as antioxidant, antimi-crobial, antilipid peroxidation, anticholinesterase, antileishmanial activities. The aim of this study was to determine chemical composition and biological activities of ethanol and n-hexane extracts of three differ-ent Jurinea species. For this purpose, different parts of J. mollis, J. cadmea and J. pontica were extracted and totally six n-hexane and six ethanol extracts were obtained. Fatty acid content of n-hexane extracts was determined by GC-FID whereas phenolic and flavonoid content of ethanol extracts by LC-HRMS. Palmitic acid (16:0) was detected as the most abundant fatty acid in all n-hexane extracts with the rates rang -ing from 42.16%-55.08%, except flowers of J. mollis (JMF) and J. cadmea (JCF). LC-HRMS analysis showed the rutin content of all extracts was higher than other flavonoids, except of J. cadmea flowers, whereas apigenin-7-glucoside was found the most abundant in JCF. Cytotoxic effects of the extracts on HeLa and HEK-293 cells were determined by MTT method, and antioxidant activities were evaluated by DPPH and CUPRAC assays. Ethanol extract ofJ. mollis flowers significantly inhibited cancerous HeLa cells, with the IC50 value of 9.683 mu g/mL while it was more less toxic on healthy HEK-293 cells. Ethanol extracts of J. mollis flowers and J. mollis steams-leaves (JMSL) showed the highest antioxidant activity by a DPPH inhibition % of 45.516 +/- 2.497 and 56.671 +/- 1.496, respectively. JMF and JMSL have also the highest CUPRAC values (0.880 +/- 0.067 and 1.085 +/- 0.152 mmol TR/g DWE, respectively). Total flavonoid content was determined using aluminum chloride colorimetric assay while total tannin and phenolic content by Folin Chiocalteu & rsquo;s reagent. Results showed that JMSL has the highest total phenolic (108.359 +/- 6.241 mg GAE/ G DWE) and flavonoid (32.080 +/- 4.385 mg QE/ g DWE) contents whereas JMF has the highest tannin content (121.333 +/- 17.889 mg TAE/ g DWE). In the light of these results, various parts of Jurinea species may be regarded as alternative sources for cytotoxic and/or antioxidant flavonoids, phenolics and unsaturated fatty acids that can arouse the interest of pharmaceutical, food and cosmetic industries. (C) 2021 Elsevier B.V. All rights reserved.Publication Metadata only Antiproliferative effects of Turkish pomegranate (Punica granatum L.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS(2021-08-01T00:00:00Z) Eroglu Ozkan, Esra; SEYHAN, MEHMET FATİH; Kurt Sirin, Ozlem; Yilmaz- Ozden, Tugba; Ersoy, Ezgi; Hatipoglu Cakmar, Seda Damla; Goren, Ahmet Ceyhan; Yilmaz Aydogan, Hulya; Ozturk, Oguz; GÖREN, AHMET CEYHANIn this study, eight different pomegranate (Punica granatum L.) cultivars from Turkey were evaluated for their antioxidant and cytotoxic effects on the MCF-7 breast cancer cell lines and MCF-10A breast fibrocystic epithelial cell lines with a focus on their chemical compositions by LC-MS/MS. Cell lines were treated with pomegranate juice extracts in different doses at selected time intervals (24th, 48th, and 72nd hour). Afterwards, WST-1 cell proliferation assay was performed to investigate the cytotoxicity of the extracts. Accordingly, all extracts decreased the cell viability of MCF-7 breast cancer cell lines and had no cytotoxic effect on the cell viability of MCF-10A cell lines. Among eight extracts, P7 (Izmir 1513), which was rich in anthocyanins such as cyanidin chloride (69.76 +/- 8.02 mu g/g extract), cyanidin-3-O-glucoside (903.66 +/- 101.89 mu g/g extract), and punicalagin (992.09 +/- 174.53 mu g/g extract), was found to demonstrate the strongest cytotoxic activity on MCF-7 breast cancer cell lines by decreasing the cell viability in half at 24th hour with an IC50 value of 49.08 mu g/ml. Practical applications Eight commercially valuable pomegranate (Punica granatum) cultivars from Turkey were examined. Pelargonidin, cyanidin, cyanidin-3-O-gl, callistephin, and delphinidin-3-O-gl were quantified. Two cultivars (P1 and P3) showed comparatively higher antioxidant effects. A cultivar (P7) showed strongest cytotoxic activity against MCF-7 breast cancer cell line. The cultivars have potential to be used as natural antioxidant and anticancer agents.Publication Metadata only Determination of dihydrocapsaicin adulteration in dietary supplements using LC-MS/MS(2020-01-01T00:00:00Z) DİNCEL, DEMET; Olgan, Hatice; Canbaloglu, Zeynep; Yalcin, Sule; Erkucuk, Aysen; TIRIS, GİZEM; GÖREN, AHMET CEYHAN; DİNCEL, DEMET; TIRIS, GİZEM; GÖREN, AHMET CEYHANAdulterations of dietary supplements are serious threats to public health in all around of world. Nowadays, in order to obtain the intended effect from natural remedies, the adulteration of natural products via artificial or harmful chemicals are increasing. A rapid LC-MS/MS method for determination of capsaicin and dihydrocapsaicin in dietary supplements via Fortis C18 (150 x 3 mm; 3 mu m) column at 25 degrees C was developed and validated in a mixture of mobile phase A (1 % formic acid solution and 0.1 % ammonium formate in water) and B (1 % formic acid solution and 0.1 % ammonium formate in methanol) with gradient program at a flow rate of 0.35 mL/min. The recoveries of developed methods weytre obtained in the range of % 95.4-106.3 for capsaicin and % 96.3-108.9 for dihydrocapsaicin. This study shows that synthetic compound dihydrocapsaicin is widely use in dietary supplements products for adulteration to increase the effects of product without any declaration.Publication Metadata only Phytochemical Screening, Antioxidant and Anticholinesterase Activity of Origanum amanum Post Endemic to Turkey(2020-01-01T00:00:00Z) Carikci, Sema; GÖREN, AHMET CEYHAN; Kilic, Turgut; Dirmenci, Tuncay; GÖREN, AHMET CEYHANThe phenolic and essential oil profiles and, antioxidant and anticholinesterase activities (AChE and BChE inhibitory activities) of Origanum amanum Post endemic to Turkey were investigated. The oil was obtained by applying hydrodistillation method and analyzed by GC-MS/MS. The plant was extracted with chloroform (C), acetone (Ac) and methanol (M). The extracts were analyzed through LC-MS/MS and the phenolic compounds were determined in quantitative amounts. Inhibition of lipid peroxidation, Cu2+ and DPPH scavenging and anticholinesterase activity of the extracts were analyzed. The main compounds of the essential oil of O. amanum were determined as cymene, carvacrol and gamma-terpinene. Rosmarinic, t-ferulic and caffeic acids were determined as the main compounds of the extracts. While the extracts did not inhibit AChE, Ac extract inhibited BChE moderately. Moreover, acetone and methanol extracts of O. amanum showed good antioxidant activity.