Guclu, Zeynep AsliHurt, Andrew PaulDonmez, NAZMİYEColeman, Nichola Jayne2019-10-052019-10-052018-07-01Guclu Z. A. , Hurt A. P. , Donmez N., Coleman N. J. , -Effect of Er:YAG laser enamel conditioning and moisture on the microleakage of a hydrophilic sealant-, ODONTOLOGY, cilt.106, ss.225-231, 2018https://hdl.handle.net/20.500.12645/3851For a given sealant, successful pit and fssure sealing is principally governed by the enamel conditioning technique and the presence of moisture contamination. A new generation of hydrophilic resin sealants is reported to tolerate moisture. This study investigates the impact of Er:YAG laser pre-conditioning and moisture contamination on the microleakage of a recent hydrophilic sealant. Occlusal surfaces of extracted human molars were either acid etched (n = 30), or successively lased and acid etched (n = 30). Ten teeth from each group were either air-dried, water-contaminated, or saliva-contaminated prior to sealing with UltraSeal XT® hydro™. Samples were inspected for penetration of fuchsin dye following 3000 thermocycles between 5 and 50 °C, and the enamel–sealant interfaces were observed by scanning electron microscopy (SEM). Signifcant diferences in microleakage were evaluated using the Mann–Whitney U test with Bonferroni adjustment (p = 0.05). Laser pre-conditioning signifcantly reduced dye penetration irrespective of whether the enamel surface was moist or dry. Microleakage of water-contaminated acid etched teeth was signifcantly greater than that of their air-dried or saliva-contaminated counterparts. SEM analysis demonstrated good adaptation in all groups with the exception of water-contaminated acid etched teeth which exhibited relatively wide gaps. In conclusion, this hydrophilic sealant tolerates the presence of saliva, although water was found to impair its sealing ability. Laser pre-conditioning signifcantly decreases microleakage in all cases.eninfo:eu-repo/semantics/openAccessArticleWOS:0004349824000018503801763210.1007/s10266-017-0323-429236177