Rider, Paul J. F.KAMIL, MOHDYilmaz, IlknurAtmaca, Habibe N.Kalkan-Yazici, MerveZiya Doymaz, MehmetKousoulas, Konstantin G.ALY, Ahmed Sayed Ibrahım2022-03-152022-03-152022-02-01http://hdl.handle.net/20.500.12645/30452Here, we present the construction of an attenuated herpes simplex virus type-1 (HSV-1)-vectored vaccine, expressing three liver-stage (LS) malaria parasite exported proteins (EXP1, UIS3 and TMP21) as fusion proteins with the VP26 viral capsid protein. Intramuscular and subcutaneous immunizations of mice with a pooled vaccine, composed of the three attenuated virus strains expressing each LS antigen, induced sterile protection against the intravenous challenge of Plasmodium yoelii 17X-NL salivary gland sporozoites. Our data suggest that this malaria vaccine may be effective in preventing malaria parasite infection using practical routes of immunization in humans.info:eu-repo/semantics/openAccessEXP1HSV-1TMP21UIS3VC2intramuscularliver stagemalaria vaccinesubcutaneousviral vectorArticleWOS:0007623097000018512483254910.3390/vaccines1002030035214758