Tekkeli, S. E. KepekciGAZIOGLU, IŞILKIZILTAS, M. V.2019-10-052019-10-052017-03-01Tekkeli S. E. K. , GAZIOGLU I., KIZILTAS M. V. , -An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization-, ACTA CHROMATOGRAPHICA, cilt.29, ss.57-65, 2017https://hdl.handle.net/20.500.12645/6969https://akjournals.com/view/journals/1326/29/1/article-p57.xmlA new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.enArticleWOS:0003943267000058501467256710.1556/1326.2017.29.1.4