Bozali K.Metin Guler E.KOÇYİĞİT A.2024-02-272024-02-272024-02-02Bozali K., Metin Guler E., KOÇYİĞİT A., "A Study on Thymoquinone: Antioxidant Capacity and Anticancer Activities in LoVo Colorectal Cancer Cells", CHEMISTRY & BIODIVERSITY, 20241612-1872https://hdl.handle.net/20.500.12645/39111Thymoquinone has antioxidant and anticancer effects. This study investigates the cytotoxic, genotoxic, and apoptotic effects of black seed and its active ingredient, thymoquinone on colorectal cancer cells. The antioxidant content of Black seed methanolic extracts (BSME) with different concentrations (50, 500 and 1000 mu g/mL) were determined by the photometric methods. The reactive oxygen production (iROS) of BSME and thymoquinone on colorectal cancer cells (LoVo) and normal epithelial cells (CCD18Co) were analyzed by the fluorometric methods. A luminometric glutathione kit was employed to observe the changes in intracellular glutathione (GSH) levels. Cytotoxicity was determined by the ATP method, genotoxicity was determined by Comet Assay, and the apoptosis was identified by the Acridine Orange/Ethidium Bromide (AO/EB) double dye method. The cytotoxicity was increased by BSME and thymoquinone in LoVo cells in a dose-dependent manner (p<0.001). BSME and thymoquinone also increased iROS, and induced apoptosis and DNA damage (p<0.001). High doses of BSME and thymoquinone on cancer and healthy cells have cytotoxic, genotoxic and apoptotic effects with pro-oxidant effects. Colorectal cancer cells are more sensitive than healthy cells.info:eu-repo/semantics/openAccessYaşam BilimleriMoleküler Biyoloji ve GenetikSitogenetikKimyaBiyokimyaAlkoloidlerTemel BilimlerLife SciencesMolecular Biology and GeneticsCytogeneticChemistryBiochemistryAlcaloidesNatural SciencesBİYOKİMYA VE MOLEKÜLER BİYOLOJİYaşam Bilimleri (LIFE)KİMYA, MULTİDİSİPLİNERTemel Bilimler (SCI)BIOCHEMISTRY & MOLECULAR BIOLOGYMOLECULAR BIOLOGY & GENETICSLife Sciences (LIFE)CHEMISTRY, MULTIDISCIPLINARYCHEMISTRYNatural Sciences (SCI)İlaç KeşfiKimya (çeşitli)Genel KimyaYapısal BiyolojiMoleküler BiyolojiKlinik BiyokimyaKanser AraştırmalarıYaşlanmaBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)Genel Biyokimya, Genetik ve Moleküler BiyolojiFizik BilimleriDrug DiscoveryChemistry (miscellaneous)General ChemistryStructural BiologyMolecular BiologyClinical BiochemistryCancer ResearchAgingBiochemistry, Genetics and Molecular Biology (miscellaneous)General Biochemistry, Genetics and Molecular BiologyPhysical SciencesArticleWOS:0011564648000018518374070910.1002/cbdv.20230188638308180