Sag, SefaİMAMOĞLU, MUSTAFASARIHAN, HALUKYULUĞ, ESİNALVER, AHMETGEZE SAATÇI, ŞÜKRANÇAY, Ali2020-10-222020-10-222020-07-01Sag S., İMAMOĞLU M., SARIHAN H., YULUĞ E., ALVER A., GEZE SAATÇI Ş., ÇAY A., -Effects of carbon dioxide pneumoperitoneum on exocrine and endocrine functions, and oxidative state of rat pancreas-, BIOTECHNIC & HISTOCHEMISTRY, 2020http://hdl.handle.net/20.500.12645/23640We investigated the effects of increased intra-abdominal pressure during laparoscopy on the endocrine and exocrine functions, oxidative stress and histopathology of the pancreas in rats. We established three experimental groups of eight animals. Group 1 was the untreated control. Forth other two groups, pneumoperitoneum with CO(2)was established for 60 min at 6 mm Hg for group 2 and 12 mm Hg for group 3; groups 2 and 3 animals were allowed to re-perfuse for 30 min. Amylase, glucagon and insulin levels were analyzed in blood samples and insulin:glucagon ratio was calculated. Histopathology and malondialdehyde assay were performed on pancreatic tissue samples. Histological damage scores for vasocongestion were increased significantly in groups 2 and 3 compared to group 1. Histological damage scores for inflammatory cell infiltration were increased significantly in group 3 compared to group 1. Malondialdehyde levels were increased significantly in group 3 compared to group 1. We found no significant differences among groups for serum amylase levels or histological damage scores for hemorrhage. Insulin and glucagon levels, and the insulin:glucagon ratio was increased significantly in group 3 compared to groups 1 and 2. We found that in rats routine laparoscopy caused increased serum insulin and glucagon levels, and histopathological changes that indicated ischemia-reperfusion injury to the pancreas.ArticleWOS:0005470867000018508784235410.1080/10520295.2020.178922432643433