İnal E.Nath E. Ö.Abudayyak M.Ulusoy Ş.İnan H. A.Cicek M.Kartal M.2023-09-182023-09-182023-12-01İnal E., Nath E. Ö., Abudayyak M., Ulusoy Ş., İnan H. A., Cicek M., Kartal M., "Chemical Composition of Different Parts of the Vitex agnus-castus L. Essential Oils and Their In-Vitro Cytotoxic Activities", RECORDS OF NATURAL PRODUCTS, cilt.17, sa.5, ss.904-917, 20231307-6167https://avesis.bezmialem.edu.tr/api/publication/03e236e7-a4df-4e55-8227-86e7ef3e777e/filehttps://hdl.handle.net/20.500.12645/38551The essential oil (EO) compositions and chemotypes of the important ethnomedicinal plantVitex agnus-castusL. flowers, leaves, and fruits collected from Türkiye (Balıkesir and İstanbul) were identified in the present study. Different parts of theV. agnus-castusEO’sin-vitrocytotoxic effects on the MCF-7 (human breast adenocarcinoma) and A549 (human lung carcinoma) human-origin cell lines were anaylzed in the current study. The composition of hydrodistiled EOs extracted from flowers, leaves, and fruits ofV. agnus-castuswere analyzed by GC–FID/MS. Monoterpene hydrocarbons and oxygenated monoterpene compounds were detected as the predominant component class of theV. agnus-castus. EOs extracted from Balıkesir region were defined as the \"α-pinene-1,8-cineole\" chemotype, while EOs extracted from İstanbul region were defined as the \"sabinene-1,8-cineole\" chemotype. Sesquiterpene hydrocarbons constituted more than 20% of the compounds in the EOs extracted from the flowers. To the best of our knowledge, this study is the first to analyze thein-vitrocytotoxic effects of flowers. This study is also the first to show thein-vitrocytotoxic effects of fruit, the most commonly used part of the plant, EO on the MCF-7 cell line. Balıkesir region’s EOs were observed as more potent -especially the purple flower’s IC50 is about 4.68 µg/mL on the MCF7 cell line- than İstanbul regions, which might be attributed to the higher amount ofα-pinene, caryophyllene, and limonene content. Our results indicated that theV. agnus-castusEOs, which containα-pinene, 1,8-cineole, caryophyllene, and limonene as major components, showed relatively high cytotoxic effects compared to the control groups on the MCF7 and A549 cell lines.info:eu-repo/semantics/openAccessEczacılıkSağlık BilimleriPharmacology and TherapeuticsHealth SciencesKlinik Tıp (MED)Yaşam Bilimleri (LIFE)Farmakoloji ve ToksikolojiClinical Medicine (MED)Life Sciences (LIFE)PHARMACOLOGY & TOXICOLOGYFarmakoloji, Toksikoloji ve Eczacılık (çeşitli)Yaşam BilimleriPharmacology, Toxicology and Pharmaceutics (miscellaneous)Life Sciencesarticle10.25135/rnp.404.2304.2761904917175