Publication: Comprehensive evaluation of phytoestrogen accumulation in plants and in vitro cultures of Medicago sativa L. -Elci- and natural tetraploid Trifolium pratense L.
No Thumbnail Available
Date
2014-01-01T00:00:00Z
Authors
Authors
Colgecen, Hatice
Caliskan, Ufuk Koca
Kartal, MURAT
Buyukkartal, Hatice Nurhan
Journal Title
Journal ISSN
Volume Title
Publisher
Metrics
Abstract
The main goal of this study was to establish callus cell suspension cultures of Medicago sativa L. -Elci- and natural tetraploid Trifolium pratense L. and to compare the isoflavone production of the cultures to the original plants. The callus culture was transferred to liquid Murashige and Skoog media (MS3 and MS5) in order to establish the cell suspension cultures. The extracts were then analyzed by liquid chromatography-mass spectroscopy (LC-MS) for their isoflavones (phytoestrogen), mainly formononetin, biochanin A, daidzein, and genistein. The production of daidzein and formononetin was higher in cell suspension culture than in callus and herba of M. sativa L. -Elci,- while biochanin A and genistein content could not be detected. On the other hand, the production of phytoestrogens was more successful in the herba of T. pratense L. than in both of the cultures. It might be suggested that T. pratense L. can be grown in larger fields, whereas M. sativa L. can be utilized to establish in vitro cultures in order to produce isoflavone compounds for pharmaceutical purposes.
Description
Keywords
Citation
Colgecen H., Caliskan U. K. , Kartal M., Buyukkartal H. N. , -Comprehensive evaluation of phytoestrogen accumulation in plants and in vitro cultures of Medicago sativa L. -Elci- and natural tetraploid Trifolium pratense L.-, TURKISH JOURNAL OF BIOLOGY, cilt.38, ss.619-627, 2014