Publication: LC-MS quantification of parthenolide and cholinesterase inhibitory potential of selected Tanacetum L. (Emend. Briq.) taxa
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2015-03-01T00:00:00Z
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Orhan, Ilkay Erdogan
Tosun, Fatma
Gulpinar, Ali Rifat
Kartal, MURAT
Duran, Ahmet
Mihoglugil, Feyyaz
Akalgan, Demet
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Abstract
The acetonitrile extracts of various Tanacetum L. (Emend. Briq.) taxa from Turkey as well as parthenolide, a sesquiterpene lactone found in Tanacetum species as active substance were investigated for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the key enzymes in pathogenesis of Alzheimer-s disease, at 100 mu g mL(-1) using ELISA microplate assay. Most of the extracts displayed a remarkable AChE inhibition where the leaf of Tanacetum argenteum subsp. flabellifolium had the highest inhibition (96.68 +/- 0.35%). The extracts had moderate inhibition toward BChE, among which the stem of Tanacetum argyrophyllum var. argyrophyllum-1 exerted the best inhibition (63.81 +/- 3.64%). However, parthenolide exhibited low inhibition against both of the enzymes. Total flavonoid content of the extracts was determined spectrophotometrically. Parthenolide, a sesquiterpene lactone, was quantified in these taxa by LC-MS and the leaf of T. argenteum subsp. argenteum possessed the richest parthenolide amount (2.261 +/- 0.002%), while most of the species screened were found to contain the required percentage (0.2% minimum) by European Pharmacopeia. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
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Orhan I. E. , Tosun F., Gulpinar A. R. , Kartal M., Duran A., Mihoglugil F., Akalgan D., -LC-MS quantification of parthenolide and cholinesterase inhibitory potential of selected Tanacetum L. (Emend. Briq.) taxa-, PHYTOCHEMISTRY LETTERS, cilt.11, ss.347-352, 2015