Publication: Chemical composition of the essential oil and hexane extract of Salvia chionantha and their antioxidant and anticholinesterase activities
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2010-11-01T00:00:00Z
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Tel, Gulsen
Ozturk, Mehmet
Mehmet, Emin Duru
Harmandar, Mansur
Topcu, GÜLAÇTI
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Abstract
The essential oil and methyl ester of hexane extract of Salvia chionantha Boiss. were analysed by GC and GC-MS. Totally, 54 components were detected in the essential oil and all of them were fully determined. Germacrene D (25.03%), beta-caryophyllene (8.71%), spathulenol (5.86%) and a-humulene (4.82%) were identified as the major compounds. In the methylated hexane extract, 3-hydroxy hexadecanoic acid (39.39%), 3-hydroxy tetradecanoic acid (12.66%) and palmitic acid (12.02%) were the major fatty acids elucidated. The antioxidant activity of the essential oil and the hexane extract was determined by using four complementary test systems: namely, beta-carotene-linoleic acid, DPPH scavenging, ABTS scavenging, and CUPRAC assays. In beta-carotene-linoleic acid assay, the extract showed 81.2 +/- 0.1% lipid peroxidation inhibition at 0.8 mg/mL concentration, while in ABTS+ assay the essential oil exhibited 77.4 +/- 0.5% inhibition at same concentration. Since, acetylcholinesterase and butyrylcholinesterase enzymes are taking place in pathogenesis of Alzheimer-s disease, in vitro anticholinesterase activity of the essential oil and the extract was also studied spectrophotometrically. At 0.5 mg/mL concentration, the essential oil showed moderate acetylcholinesterase (56.7 +/- 1.9%) and butyrylcholinesterase (41.7 +/- 2.9%) inhibitory activity, while the extract was only exhibited activity (63.1 +/- 0.8%) against butyrylcholinesterase enzyme. Hence, the essential oil may be useful as a moderate anticholinesterase agent, particularly against acetylcholinesterase. (C) 2010 Elsevier Ltd. All rights reserved.
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Tel G., Ozturk M., Mehmet E. D. , Harmandar M., Topcu G., -Chemical composition of the essential oil and hexane extract of Salvia chionantha and their antioxidant and anticholinesterase activities-, FOOD AND CHEMICAL TOXICOLOGY, cilt.48, ss.3189-3193, 2010