Publication: Oligomer based real-time detection of microorganisms producing nuclease enzymes
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Date
2019-02-21T00:00:00Z
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Mozioglu, Erkan
Akyurek, Sema
Gunduz, Simay
Akgoz, Muslum
Goren, Ahmet C.
Kocagoz, Tanil
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Abstract
In this study, we provide a method using fluorescently labeled oligonucleotides for the diagnosis of microorganisms producing nucleases in real time, while growing them in culture media. The detection of such microorganisms was possible in a short period of time, as short as 10 minutes up to a maximum of 8 hours, depending on the bacterial density. We also showed the suitability of this new method for determination of minimum inhibitory concentration (MIC) in culture media in a very short period of time, compared to conventional methods. We believe that it can make a significant contribution to gain new insights for analysis of complex materials such as clinical samples, food samples and environmental samples.
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Mozioglu E., Akyurek S., Gunduz S., Akgoz M., Goren A. C. , Kocagoz T., -Oligomer based real-time detection of microorganisms producing nuclease enzymes-, ANALYST, cilt.144, ss.1379-1385, 2019