Publication:
Spectrofluorimetric determination of benidipine in pharmaceutical preparation and spiked plasma samples using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole

No Thumbnail Available
Date
2019-01-01T00:00:00Z
Authors
TEKKELİ, ŞERİFE EVRİM
Onal, Cem
Journal Title
Journal ISSN
Volume Title
Publisher
Research Projects
Organizational Units
Journal Issue
Abstract
A new spectrofluorimetric technique was produced for the analysis of benidipine (BDP) in tablets and spiked plasma samples. The developed method was based on coupling between Benidipine, (a secondary amine group), and 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) forming fluorescent dervatived as a NBD-Benidipine. The reaction was occured by using pH 8.5 buffer solution to form fluorescent derivatives that are measured lambda(em): 550 nm and lambda(ex): 465 nm. A variable parameters effecting on the derivatization process were studied. The calibration graph was linear in the range of 10-200 ng mL(-1). LOD and LOQ values were a 0.445 ng mL(-1) and 1.348 ng mL(-1), respectivelly. The developed method was applied to BDP in commercially available film tablets. An average recovery was found as 99.80% without interference from the available excipients. Besides, the fluorimetric technique was also succesfully applied to spiked human plasma.
Description
Keywords
Citation
Onal C., TEKKELİ Ş. E. , -Spectrofluorimetric determination of benidipine in pharmaceutical preparation and spiked plasma samples using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole-, JOURNAL OF RESEARCH IN PHARMACY, cilt.23, ss.1060-1066, 2019
Sustainable Development Goals