Person: GÜNEŞER, MEHMET BURAK
15 results
Search Results
Now showing 1 - 10 of 15
Publication Metadata only Comparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigants(2015-02-01T00:00:00Z) Eldeniz, Ayce Unverdi; Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; GÜNEŞER, MEHMET BURAKThe aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.Publication Metadata only Evaluation of antibacterial effects of contemporary orthodontic bonding materials(2017-01-01) Baka, Zeliha Muge; Eldeniz, Ayce Unverdi; Guneser, MEHMET BURAK; Basciftci, Faruk Ayhan; GÜNEŞER, MEHMET BURAKAim: The aim of this study was to evaluate the antibacterial effects of five orthodontic bonding materials commonly used in orthodontics. Materials and Methods: The antibacterial effects of five orthodontic bonding materials were evaluated against Streptococcus mutans and Streptococcus salivarius by direct contact test (DCT). Using the DCT technique, quintet specimens of Transbond XT, Blugloo, Grengloo, Light Bond, and Opal Bond were applied to the bottom and the walls of the five wells of a 96-microtiter plate with a height of 2 mm. Samples were prepared in two sets: 1-day samples and 7-day samples. Ten microliters of bacterial suspension were added to each well for direct contact with each material for 1h at 37 degrees C. Bacterial growth was then measured in a microplate spectrophotometer hourly at 600 nm for 24h. Five uncoated wells with identical inoculum sizes served as positive controls. The data obtained at the end of 24h was statistically analyzed with one-way ANOVA, and post hoc comparisons were done using Tamhane-s T2 test. Results: Blugloo generally showed better antibacterial activity than the other materials. Transbond XT did not show any antibacterial activity. There was a statistically significant difference between Transbond XT and Light Bond in the 1-day sample against S. mutans (p0.05). Conclusion: Blugloo had the most potent antibacterial activity against S. salivarious.Publication Metadata only Effect of Various Endodontic Irrigants on the Push-out Bond Strength of Biodentine and Conventional Root Perforation Repair Materials(2013-03-01T00:00:00Z) Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAKIntroduction: The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fosses, France) in comparison with contemporary root perforation repair materials. Methods: Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to-be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. Results: Biodentine showed significantly higher push-out bond strength than MTA (P amalgam >= IRM >= Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. Conclusions: Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin. (J Endod 2013;39:380-384)Publication Metadata only Effects of fruit vinegars on root dentin microhardness and roughness.(2019-01-01) AKBULUT, MB; Guneser, MEHMET BURAK; ELDENIZ, AU; GÜNEŞER, MEHMET BURAKPublication Metadata only Comparison of Conventional Syringe, CanalBrush, EndoActivator, Photon-Induced Photoacoustic Streaming, and Manual Instrumentation in Removing Orange-Brown Precipitate: An In Vitro Study(2017-06-01) Dincer, ASİYE NUR; Guneser, MEHMET BURAK; ARSLAN, Dilara; GÜNEŞER, MEHMET BURAK; DİNÇER, ASİYE NURObjective: The aim of this in vitro study was to compare the various techniques for removing precipitate formed after irrigation with sodium hypochlorite (NaOCl) and chlorhexidine (CHX). Background data: It is still unclear whether different irrigant activation systems can effectively remove precipitate that is firmly attached to the root canal walls. Methods: Eighty-two extracted single-rooted human teeth were instrumented by using the ProTaper rotary system. The roots were irrigated with 5% NaOCl and then 2% CHX to form orange-brown precipitate on the root canal walls. The teeth were sectioned longitudinally, and the amount of precipitate on the canal walls was measured under a stereomicroscope at 15xmagnification before the root halves were reassembled. Then, conventional syringe irrigation (CSI), CanalBrush (CB), EndoActivator (EA) system, photon-induced photoacoustic streaming (PIPS), and manual instrumentation (MI) techniques were used to remove the precipitate, during which 5mL of distilled water was used for 1min. The amount of precipitate remaining on the canal walls was measured, and the percentage of precipitate removed was calculated. Data were analyzed by using the Kruskal-Wallis and Mann-Whitney U tests (p=0.05). Results: All experimental groups contained residual precipitate. The precipitate was more effectively removed in the MI group than in the other groups (p0.05). Conclusions: None of the techniques used was able to completely remove the orange-brown precipitate from the root canal surfaces.Publication Open Access Do the intracanal medicaments affect the marginal adaptation of calcium silicate-based materials to dentin?(2019-06-01T00:00:00Z) Dincer, ASİYE NUR; Ozturk, TY; Guneser, MEHMET BURAK; Taschieri, S; Maddalone, M; Venino, PM; Del, Fabbro; GÜNEŞER, MEHMET BURAK; DİNÇER, ASİYE NURPublication Metadata only Pulp tissue dissolution capacity of QMix 2in1 irrigation solution.(2015-07-01) ARSLAN, D; Guneser, MEHMET BURAK; KUSTARCI, A; ER, K; SISO, SH; GÜNEŞER, MEHMET BURAKPublication Metadata only Are dentin posts biomechanically intensive?: A laboratory and FEA study(2014-12-17) Belli, Sema; Celik, Kezban; Akbulut, Makbule Bilge; Guneser, MEHMET BURAK; Eraslan, Oguz; Eskitaccioglu, Gurcan; GÜNEŞER, MEHMET BURAKThe goal of this laboratory and finite elemental stress analysis (FEA) study was to compare fracture strength of dentin post (DP) with stainless steel and glass fiber posts. Single-rooted teeth were decoronated and restored with ParaPost (PP), i-TFC (TFC), and DPs (n=10). Resin cores were created and loaded until failure (1.0mm/min). The data were analyzed (ANOVA, Tukey, Chi-Square tests). Three-dimensional FEA models of the posts were created and the stress distributions were calculated using Solid Works/Cosmos works structural analysis program. A significant difference was found among the groups (p<0.001). TFC group showed the highest and the DP group showed the lowest fracture resistance (p<0.001). PP created more stress at the root dentin and inside the post. Restoration of roots with glass fiber and DPs may reduce stresses within the core material and the remaining root. However, a fracture-resistant restoration cannot be achieved by using a DP.Publication Open Access The effect of gelatinase production of <i>Enterococcus faecalis</i> on adhesion to dentin after irrigation with various endodontic irrigants.(2016-11-15) Guneser, MEHMET BURAK; ELDENIZ, AU; GÜNEŞER, MEHMET BURAKObjectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E. faecalis. After incubation of the discs with the bacterial suspensions aerobically for 48 h, XTT assay was conducted for bacterial adherence evaluation. Data were statistically analyzed by ANOVA and Tukey's HSD tests (p = .05). Results: Gelatinase-producing E. faecalis adhered to dentin was significantly more than gelatinase-deficient E. faecalis in all test groups (p < .05). Adherence to CHX-treated dentin was lower than to the surfaces treated with other irrigants, alone or in combination (p < .05). These differences were significant except for comparisons with QMix for gelatinase-producing bacteria (p < .05). Conclusions: Gelatinase production of E. faecalis may be an important factor for bacterial adhesion. The addition of CHX to the irrigation regimen resulted in fewer adhered bacteria to dentin. QMix was not as effective as CHX in terms of bacterial adhesion prevention.Publication Open Access Compressive strength, surface roughness, and surface microhardness of principle tricalcium silicate-based endodontic cements after universal adhesive application(2022-03-01T00:00:00Z) Olcay, K; Guneser, MEHMET BURAK; Dincer, A N; Uyan, H M; GÜNEŞER, MEHMET BURAKAims and background: It was aimed to evaluate compressive strength (CS), surface roughness, and microhardness of mineral trioxide aggregate (ProRoot MTA) and Biodentine (BD) after adhesive application. Materials and methods: Tests was carried out according to international ISO standard. ProRoot MTA and BD were prepared in Teflon molds according to manufacturer's instructions: n = 210 for CS; n = 210 for microhardness. Samples were incubated for 7 days at 37°C in 100% humidity. Surfaces were smoothed with up to 2000 grits of silicon-carbide sandpaper on abrasive device at 150 rpm, randomly divided into seven groups (n = 15). Clearfil Universal Bond, All Bond Universal, and Single Bond Universal (SBU) were applied in both total-etch and self-etch (SE) modes. Adhesives were applied according to manufacturers' recommendations (no adhesive used in control). CS was performed at speed of 1 mm/min, microhardness at 100 gr for 15 s. The surface roughness of the samples was analyzed with atomic force microscopy. Two-way analysis of variance and post hoc Tukey tests were used for the evaluation of the data. Results: Man CS and microhardness values between ProRoot MTA and BD were as follows: 24.9 N, 72.6 HV; 59.8 N, 59.0 HV, respectively. In CS, BD was higher than ProRoot MTA (P < 0.05). In other comparisons except for SBU SE group (P < 0.05), BD and ProRoot MTA showed similar results (P > 0.05). However, ProRoot MTA was found higher than BD regarding microhardness (P < 0.05). As a result of the adhesive application in both BD and ProRoot MTA groups, a decrease in surface roughness was observed compared to the control group. Conclusion: BD exhibited better results than ProRoot MTA regarding CS. However, ProRoot MTA was found to be more successful than BD in terms of microhardness. BD and ProRoot MTA showed similar physical properties in terms of surface roughness. To improve regenerative procedures, besides the selection of bioceramic cements, the interaction between cements and materials applied during coronal restoration should be considered.