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GÜNEŞER, MEHMET BURAK

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MEHMET BURAK
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GÜNEŞER
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Now showing 1 - 8 of 8
  • PublicationMetadata only
    Comparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigants
    (2015-02-01T00:00:00Z) Eldeniz, Ayce Unverdi; Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; GÜNEŞER, MEHMET BURAK
    The aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.
  • PublicationMetadata only
    Evaluation of antibacterial effects of contemporary orthodontic bonding materials
    (2017-01-01) Baka, Zeliha Muge; Eldeniz, Ayce Unverdi; Guneser, MEHMET BURAK; Basciftci, Faruk Ayhan; GÜNEŞER, MEHMET BURAK
    Aim: The aim of this study was to evaluate the antibacterial effects of five orthodontic bonding materials commonly used in orthodontics. Materials and Methods: The antibacterial effects of five orthodontic bonding materials were evaluated against Streptococcus mutans and Streptococcus salivarius by direct contact test (DCT). Using the DCT technique, quintet specimens of Transbond XT, Blugloo, Grengloo, Light Bond, and Opal Bond were applied to the bottom and the walls of the five wells of a 96-microtiter plate with a height of 2 mm. Samples were prepared in two sets: 1-day samples and 7-day samples. Ten microliters of bacterial suspension were added to each well for direct contact with each material for 1h at 37 degrees C. Bacterial growth was then measured in a microplate spectrophotometer hourly at 600 nm for 24h. Five uncoated wells with identical inoculum sizes served as positive controls. The data obtained at the end of 24h was statistically analyzed with one-way ANOVA, and post hoc comparisons were done using Tamhane-s T2 test. Results: Blugloo generally showed better antibacterial activity than the other materials. Transbond XT did not show any antibacterial activity. There was a statistically significant difference between Transbond XT and Light Bond in the 1-day sample against S. mutans (p0.05). Conclusion: Blugloo had the most potent antibacterial activity against S. salivarious.
  • PublicationMetadata only
    Effect of Various Endodontic Irrigants on the Push-out Bond Strength of Biodentine and Conventional Root Perforation Repair Materials
    (2013-03-01T00:00:00Z) Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAK
    Introduction: The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fosses, France) in comparison with contemporary root perforation repair materials. Methods: Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to-be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. Results: Biodentine showed significantly higher push-out bond strength than MTA (P amalgam >= IRM >= Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. Conclusions: Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin. (J Endod 2013;39:380-384)
  • PublicationOpen Access
    Tooth discoloration effects of calcium silicate based barrier materials used in revascularization and treatment with internal bleaching
    (2017-12-01T00:00:00Z) Akbulut, Makbule Bilge; Terlemez, Arslan; Akman, Melek; Buyukerkmen, Begum; Guneser, Mehmet Burak; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAK
    Background/purpose: Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this in vitro study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration.
  • PublicationMetadata only
    Fracture resistance of roots obturated with a novel calcium silicate-based endodontic sealer (BioRoot RCS)
    (2016-01-01) Guneser, MEHMET BURAK; Akman, Melek; Kolcu, Inci Baser; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAK
    The aim of this ex vivo study was to evaluate the vertical-fracture resistance of roots obturated with a newly developed tricalcium silicate cement (BioRoot RCS; Septodont, Saint Maur Des Fosses, France) using cold lateral compaction technique (LC) or matched-taper single-cone gutta-percha technique (SC). Decoronated 82 single-rooted mandibular premolars were chemo-mechanically prepared and then randomly divided into 6 experimental groups (n = 12) and 2 control groups (n = 5): Group 1 iRoot SP-LC; Group 2 iRoot SP-SC; Group 3 MTA Fillapex-LC; Group 4 MTA Fillapex-SC; Group 5 BioRoot RCS-LC and Group 6 BioRoot RCS-SC. In the positive-control group, roots were instrumented but not filled, and in the negative-control group, roots were neither instrumented nor filled. All samples were incubated for two weeks and then subjected to vertical loading force (1 mm/min) until fracture. The force required to fracture each specimen were determined, and the data were statistically analyzed. The highest significant fracture resistance was recorded for the iRoot SP-LC, iRoot SP-SC, BioRoot RCS-LC, and BioRoot RCS-SC groups, with no significant difference among them (p > 0.05) when compared with the positive-control group (p < 0.05), whereas the lowest significant values were observed in the MTA Fillapex-LC and MTA Fillapex-SC groups (p < 0.05). The vertical fracture resistance of roots obturated with BioRoot RCS and iRoot SP sealers using either LC or SC technique was found to be similar to that of intact teeth. BioRoot RCS, newly developed tricalcium silicate cement, might have the potential to reinforce the instrumented teeth against vertical root fracture.
  • PublicationMetadata only
    Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs
    (2016-03-03) Akman, Melek; Akbulut, Makbule Bilge; Guneser, MEHMET BURAK; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAK
    To evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole-ciprofloxacin-cefaclor, Group2: a combination of metronidazole-ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P=0.00). The medications have an effect on the push-out bond strength of both materials (P=0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.
  • PublicationOpen Access
    Antibacterial effect of chlorhexidine-cetrimide combination, Salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants
    (2016-10-01) Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAK
    The aim of the present study was to compare the antimicrobial effect of sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), a CHX/cetrimide solution (CHX+CTR), octenidine hydrochloride (OCT) and Salvia officinalis plant extract against Enterococcus faecalis. Seventy decoronated single-rooted human teeth were infected and divided into 6 test (n=10) and 2 control groups (n=5) (negative, sterile samples and positive, infected samples). Following irrigants were then applied to test groups: 2.5% NaOCl, 5.25% NaOCl, CHX, CHX+CTR, S. officinalis extract and OCT. The dentin chips were obtained from inner root canal walls and analyzed by counting the number of colony forming units (CFU). The 2.5% NaOCl, 5.25% NaOCl, CHX and OCT groups presented no bacterial growth (CFU=0). S. officinalis and CHX+CTR groups reduced the number of E. faecalis cells but could not eliminate all. OCT may have potential as an endodontic irrigant in treatment of infected root canals.
  • PublicationOpen Access
    Push-out bond strength of a new post system after various post space treatments
    (2016-12-01) Akman, Melek; Eldeniz, Ayce Unverdi; Ince, Selen; Guneser, MEHMET BURAK; GÜNEŞER, MEHMET BURAK
    To assess the effect of post-space treatment with chelating agents on the push-out bond-strength of a glass fiber post-system. Fortyeight human teeth were decoronated. The roots were prepared to size 40 and obturated. The post-spaces were prepared with PeesoReamer drills. The post-spaces were irrigated with (i) NaOCl and EDTA with chlorhexidine (QMix), NaOCl and EDTA, NaOCl and Citric acid, (iv) NaOCl and saline as a control group. i-TFC glass fiber posts were then luted with i-TFC bond system. The samples were horizontally sectioned. The displacement resistance was measured. Push-out bond-strength (MPa) was calculated. Data were analyzed. NaOCl/QMix group showed highest values to dentine whereas NaOCl/Citric acid group showed lowest values. i-TFC post-system demonstrated equal bond strength values when post-space treated with either NaOCl/EDTA or NaOCl/Saline. It can be concluded that post-space could be treated with NaOCl and QMix in order to increase adhesion of i-TFC post-system to root dentine.