Person: ÖZTEN KANDAŞ, NUR
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Publication Metadata only Comparison of laser and ozone treatments on oral mucositis in an experimental model(2017-04-01T00:00:00Z) BAYER, SUZAN; Kazancioglu, Hakki Oguz; Acar, Ahmet Huseyin; Demirtas, Nihat; ÖZTEN KANDAŞ, NUR; BAYER, SUZAN; ÖZTEN KANDAŞ, NUROral mucositis (OM) induces severe pain and limits fundamental life behaviors such as eating, drinking, and talking for patients receiving chemotherapy or radiotherapy. In addition, through opportunistic microorganisms, OM frequently leads to systemic infection which then leads to prolonged hospitalization. Severe lesions often adversely affect curative effects in cancer cases. Therefore, the control of OM is important for oral health quality of life and prognosis. Low-level laser therapy (LLLT) and ozone may be useful to accelerate wound healing. In this study, 24 Sprague-Dawley rats were divided into three groups as control, ozone, and laser groups. All groups received 5-fluorouracil intraperitoneally and trauma to the mouth pouch with a needle. After the formation of OM in the mouth, the control group had no treatment; the ozone group was administered ozone, and the laser group, LLLT. Then, all groups were sacrificed and basic fibroblast growth factor (bFGF), transforming growth factor (TGF-beta), and platelet-derived growth factor (PDGF) were evaluated in all groups. LLLT was determined to be statistically significantly more effective than ozone on FGF and PDGF. However, in respect of TGF-beta, no statistically significant difference was observed between the groups. In conclusion, within the limitations of this study, LLLT is more effective than ozone. However, further studies on this subject are required.Publication Metadata only Mikroenkapsüle edilen paratiroid hücrelerinin in-vitro optimizasyonu(2017-12-01) YUCESAN, Emrah; GONCU, Beyza; BAŞOĞLU, HARUN; ÖZTEN KANDAŞ, NUR; ERSOY, YELİZ EMİNE; AKBAŞ, FAHRİ; AYŞAN, MUSTAFA ERHAN; BAŞOĞLU, HARUN; GÖNCÜ, BEYZA SERVET; ÖZTEN KANDAŞ, NUR; ERSOY, YELIZ EMINE; AKBAŞ, FAHRİ; AYŞAN, MUSTAFA ERHANPublication Open Access Cancer chemoprevention research with selenium in the post-SELECT era: Promises and challenges.(2016-01-01) LÜ, J; ZHANG, J; JIANG, C; DENG, Y; Özten, NUR; BOSLAND, MC; ÖZTEN KANDAŞ, NURThe negative efficacy outcomes of double-blinded, randomized, placebo-controlled Phase III human clinical trials with selenomethionine (SeMet) and SeMet-rich selenized-yeast (Se-yeast) for prostate cancer prevention and Se-yeast for prevention of non-small cell lung cancer (NSCLC) in North America lead to rejection of SeMet/Se-yeast for cancer prevention in Se-adequate populations. We identify two major lessons from the outcomes of these trials: 1) The antioxidant hypothesis was tested in wrong subjects or patient populations. 2) The selection of Se agents was not supported by cell culture and preclinical animal efficacy data as is common in drug development. We propose that next-generation forms of Se (next-gen Se), such as methylselenol precursors, offer biologically appropriate approaches for cancer chemoprevention but these are faced with formidable challenges. Solid mechanism-based preclinical efficacy assessments and comprehensive safety studies with next-gen Se will be essential to re-vitalize the idea of cancer chemoprevention with Se in the post-SELECT era. We advocate smaller mechanism-driven Phase I/II trials with these next-gen Se to guide and justify future decisions for definitive Phase III chemoprevention efficacy trials.Publication Open Access Synthesis and biological evaluation of novel coumarin-chalcone derivatives containing urea moiety as potential anticancer agents(2017-12-01) ZENGİN KURT, BELMA; ÖZTEN KANDAŞ, NUR; DAĞ, AYDAN; SÖNMEZ, FATİH; KÜÇÜKİSLAMOĞLU, MUSTAFA; ÖZTEN KANDAŞ, NURPublication Metadata only Interactive effects of 9-cis-retinoic acid and androgen on proliferation, differentiation, and apoptosis of LNCaP prostate cancer cells.(2017-01-01) ESKRA, JN; KUIPER, JW; WALDEN, PD; BOSLAND, MC; Özten, NUR; ÖZTEN KANDAŞ, NURPublication Metadata only Achieving the balance: Biphasic effects of genistein on PC-3 cells(2019-08-01T00:00:00Z) Goncu, BEYZA; Yildiz, Mehmet Taha; Ozten-Kandas, Nur; GÖNCÜ, BEYZA SERVET; ÖZTEN KANDAŞ, NURThis study examined the response of PC-3 cells to physiological (0.5, 2.5, 5, 10 mu M) and pharmacological (50 mu M) concentrations of genistein which is a main bioactive compound in soy. Following 48 hr genistein treatment, cell-based assays and genome-wide microarray were performed. It was evidenced that maximal physiologically achievable concentrations of genistein (0.5-10 mu M) lead to significant increase in cell viability (p 10 mu M) appeared to have a novel mechanism of action, specifically down-regulating TGF-beta by decreasing specifically SMAD 2/3,4 which are in the downstream TGF-beta signaling cascade. Practical applications This study highlights for the first time that maximal physiologically achievable concentrations of genistein (0.5-10 mu M) have proliferative effects evidenced by alterations in global gene expression patterns of PC-3 cells. Our results particularly represent a closer examination of dietary genistein consumption for the prevention and/or treatment of cancer that maximal physiologically achievable concentrations of genistein could have detrimental effects on individuals with prostate cancer. Further studies as in vivo would be necessary to remove shadows on the effect of genistein on prostate cancer progression.Publication Metadata only Pharmacogenetics: Role of Single Nucleotide Polymorphisms(2019-01-01T00:00:00Z) YÜCESAN, Emrah; ÖZTEN KANDAŞ, NUR; ÖZTEN KANDAŞ, NURGenome sequencing methods have basically similar algorithms, although they show a few differences between the platforms. The human genome contains approximately three billion base pairs, and this amount is huge and therefore impossible to sequence at one step. However, this amount is not a problem for developed technology. Researchers break DNA into small random pieces and then sequence and reassemble. Library preparation, sequencing, bioinformatic approaches and reporting. High-quality library preparation is critical and the most important part of the next-generation sequencing workflow. Successful sequencing directly requires high-quality libraries. Sequencing is second step and all high-throughput sequencing approaches are generally based on conventional Sanger sequencing. After preparation of library and sequencing, later steps are completely computer-based (in silico) approaches called as bioinformatics.Publication Metadata only Achieving the balance: Biphasic effects of genistein on PC-3 cells(2019-06-01) TERZİOĞLU, ŞULE; Goncu, Beyza; Ozten Kandas, NUR; TERZİOĞLU, ŞULE; ÖZTEN KANDAŞ, NURPublication Open Access Differential anti-proliferative and apoptotic effects of lichen species on human prostate carcinoma cells(2020-09-01T00:00:00Z) Goncu, Beyza; SEVGİ, ECE; KIZILARSLAN HANÇER, ÇAĞLA; Gokay, Guzin; ÖZTEN KANDAŞ, NUR; GÖNCÜ, BEYZA SERVET; SEVGİ, ECE; KIZILARSLAN HANÇER, ÇAĞLA; ÖZTEN KANDAŞ, NURLichens are stable symbiotic associations between fungus and algae and/or cyanobacteria that have different biological activities. Around 60% of anti-cancer drugs are derived from natural resources including plants, fungi, sea creatures, and lichens. This project aims to identify the apoptotic effects and proliferative properties of extracts ofBryoria capillaris (Ach.) Brodo&D.Hawksw,Cladonia fimbriata (L.) Fr.,Evernia divaricata (L.) Ach.,Hypogymnia tubulosa (Schaer.) Hav.,Lobaria pulmonaria (L.) Hoffm., andUsnea florida (L.) Weber ex Wigg. lichen species on prostate cancer cells. Lichen extracts were performed by ethanol, methanol, and acetone separately by using the Soxhlet apparatus and the effects of the extracts on cell viability, proliferation, and apoptosis were measured with the utilization of MTT, LDH assay, Annexin V assay, and Western Blot. Findings of our study revealed a positive correlation between the elevation of cell sensitivity and the increase in the treatment doses of the extract in that higher doses applied reverberate to higher cell sensitivity. A similar correlation was also identified between cell sensitivity elevation and the duration of the treatment. Evidence in our study have shown the existence of an anti-proliferative effect in the extracts ofBryoria capillaris,Evernia divaricata (L.) Ach.,Hypogymnia tubulosa (Schaer.) Hav.,Lobaria pulmonaria (L.) Hoffm., andUsnea florida (L.) Weber ex Wigg., while a similar effect was not observed in the extracts ofCladonia fimbriata.Evernia divaricatainduced anti-proliferative and apoptotic effects in PC-3 cells,whichinduced apoptotic cell death by both extrinsic and intrinsic pathways.Hypogymnia tubulosahas been shown to have anti-proliferative and apoptotic effects in all extractions methods and our findings identified that both the percentage of the apoptotic cells and apoptotic protein expressions recorded an increase at lower treatment concentrations. AlthoughLobaria pulmonariais known to have significant cytotoxic effects, we did not observe a decrease in cell proliferation. Indeed, proliferation marker proliferating cell nuclear antigen (PCNA) protein expression levels have shown an increase in all extracts, whileUsnea florida exhibitedapoptosis induction and slight proliferation reduction in extract treatments with lower concentrations. We tested 18 extracts of six lichen species during our study. Of these,Evernia divaricataandHypogymnia tubulosademonstrated significant apoptotic activity on prostate cancer cells including at low concentrations, which implies that it is worth pursuing the biologically active lead compounds of these extracts on prostate cancerin vitro. Further corroboratory studies are needed to validate the relative potential of these extracts as anti-metastatic and anti-tumorigenic agents.Publication Metadata only Comparison of the efficacy of low-level laser therapy and photodynamic therapy on oral mucositis in rats(2019-09-01T00:00:00Z) Alinca, Suzan Bayer; Saglam, Ebru; ÖZTEN KANDAŞ, NUR; Okcu, Oguzhan; YILMAZ, NERGİZ; Doğan, Mehmet Ali; SAĞLAM, EBRU; ÖZTEN KANDAŞ, NUR; GÖNCÜ, BEYZA SERVETCancer treatment with chemotherapy or radiotherapy is associated with some side effects including in the oral cavity. One of the more significant oral complications is oral mucositis (OM) which induces severe pain and limits fundamental life behaviors such as eating, drinking, and talking. Although advancements in cancer treatment improved the survival rate, severe OM and opportunistic infection affect treatment adversely. Therefore, the control of OM is important for oral health quality of life and prognosis. Low-level laser therapy (LLLT) and photodynamic therapy (PT) are noninvasive methods that reduce inflammation and pain during wound healing. The aim of this study is to evaluate immunohistochemical and histological examination of the OM region of the PT comparing LLLT. In this study, 24 Sprague-Dawley rats were divided into three groups as control, LLLT, and PT groups. All groups received 5-fluorouracil intraperitoneally and a linear trauma to the mouth pouch with a needle. After the formation of OM in the mouth, the control group had no treatment; the LLLT group was administered LLLT, and the PT group had LLLT after indocyanine green application. Then all groups were sacrificed, and histological analyses and protein level detection of basic fibroblast growth factor (bFGF), transforming growth factor (TGF-beta), and platelet-derived growth factor (PDGF-BB) were evaluated in all groups. PT was determined to be more statistically significantly than LLLT with bFGF and PDGF-BB. However, regarding TGF-beta, no statistically significant difference was observed between the groups. Within the limitations of this study, indocyanine green may accelerate the LLLT effect. However, further studies on this subject are required.