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Now showing 1 - 8 of 8
  • PublicationMetadata only
    Investigation the fatty acid profile of commercial black cumin seed oils and seed oil capsules: Application to real samples
    (2019-07-01T00:00:00Z) Isik, Selin; Erdem, Sinem Aslan; KARTAL, MURAT; KARTAL, MURAT
    Medicinal and aromatic plants are widely used around the world. Today, Black cumin ( Nigella sativa L.), one of the most popular medicinal plants, offers numerous benefits for human health. Black cumin oil has known to be used for the treatment of asthma, hypertension, cancer, rheumatism, and headaches over the course of many years. In this study, ten different black cumin seed oils and seed oil capsules, which have been obtained from local markets in Turkey, were investigated for their fatty acid profiles as percentages via gas chromatography and mass spectroscopy (GC-MS). According to the results obtained, the major fatty acids found in capsules and oil samples were similar with small differences in terms of amount; linoleic, oleic and palmitic acids were found as major compounds; 35.27-58.15%, 25.06-51.83%, 7.29-12.88 % in oil samples; 36.67-56.61%, 24.93-46.71%, and 7.90-12.71% in seed oils capsule samples, respectively. This is the first study determining the fatty acid percentages of commercial black cumin seed oil and seed oil capsules.
  • PublicationMetadata only
    Contractile Effects of Eryngium kotschyi Boiss. on Rat Isolated Ileum and Detrussor Muscle
    (2014-09-01T00:00:00Z) Baydan, Emine; Kartal, MURAT; Yurdakok, Begum; Erdem, Sinem Aslan; Ince, Sinan; EKICI, Husamettin; Alp, Harun; KARTAL, MURAT
    The pharmacological activity of the aerial (EKA) and root (EKR) parts of the endemic plant, Eryngium kotschyi Boiss., on rat isolated ileum and detrusor muscle was investigated. Plant extracts alone and with the presence of agonist (acethylcholine) and antagonist (atropin, verapamile, oxybutinine-detrusor muscle, and papaverine-ileum) drugs, along with Ca2+ applications on calcium-free medium, were applied. Plant extracts induced contraction in ileum and detrusor muscle where the contractions were concentration dependant for EKA and EKR single dose applications in detrusor muscle and concentration-free contractions were observed in cumulative applications for both tissues. Aerial and root parts of Eryngium extracts induced contractions in dose, tissue and protocol dependent manner where the contractions were affected by the tested antagonists, which could be attributed to non-specific pathways including calcium ions and calcium channel stimulations.
  • PublicationMetadata only
    Effect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds
    (2018-07-01T00:00:00Z) Rezaei, Fereshteh; Isik, Selin; KARTAL, MURAT; Erdem, Sinem Aslan; KARTAL, MURAT
    The aim of this study is to analyze the changes on thymoquinone content, which is the major constituent of black cumin (Nigella sativa L.) seeds, by using priming methods. For the priming studies, seeds placed in distilled water (18 - 20 hours), PEG (Polyethylene glycol 10%, 20%) and mannitol (4% and 6%) for 20 hours and subsequently dried for 24 hours on blotting paper at room temperature (24 degrees C +/- 1 degrees C). The best priming results were obtained in the 4% of mannitol treatment. Control (not primed) Nigella seeds and 4% of mannitol primed seeds were planted in both greenhouse and farm conditions. Seed extracts were analyzed with HPLC to compare the amount of thymoquinone. The results reveal that seed priming with 4% of mannitol increases the amount of thymoquinone in Nigella sativa. In addition, the effects of plant growth hormones on callus regeneration of these primed seeds were investigated herein. (C) 2018 ACG Publications. All rights reserved.
  • PublicationMetadata only
    Exploration of cholinesterase and tyrosinase inhibitory, antiprotozoal and antioxidant effects of Buxus sempervirens L. (boxwood)
    (2012-11-01T00:00:00Z) Orhan, Ilkay Erdogan; Erdem, Sinem Aslan; Senol, Fatma Sezer; Kartal, MURAT; Sener, Bilge; KARTAL, MURAT
    The petroleum ether, chloroform, ethyl acetate, and methanol extracts of the aerial parts of Buxus sempervirens growing naturally in Turkey were investigated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as well as tyrosinase (TYRO) enzymes for their inhibitory activity using ELISA microplate reader. Antiprotozoal activity of the extracts was tested against the parasites; Plasmodium falciparum (malaria) and Trypanosoma brucei rhodesiense (human African trypanosomiasis) at 0.81 and 4.85 mu g/mL concentrations. As antioxidant activity contributes to antimalarial activity, the extracts were also tested for their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, iron-chelating capacity, and ferric-reducing antioxidant power (FRAP). Total phenol and flavonoid contents in the extracts were determined spectrophotometrically. The alkaloid fraction of the chloroform extract afforded two major alkaloids named (+)-buxabenzamidienine (1) and (+)-buxamidine (2). Our results showed that, except for the petroleum ether extract, the extracts displayed high antimalarial and anti-BChE effects as well as quite high iron-chelation capacity. Therefore, we suggest that the active extracts in the antimalarial tests might be showing their effects through the mechanism of BChE inhibition and additionally iron-chelation ability. (C) 2012 Elsevier B.V. All rights reserved.
  • PublicationMetadata only
    Tyrosinase and Cholinesterase Inhibitory Potential and Flavonoid Characterization of Viola odorata L. (Sweet Violet)
    (2015-09-01T00:00:00Z) Orhan, Ilkay Erdogan; Senol, Fatma Sezer; Erdem, Sinem Aslan; Tatli, I. Irem; Kartal, MURAT; Alp, Şevket; KARTAL, MURAT
    Inhibitory potential of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Viola odorata L. (VO) was investigated against tyrosinase (TYR) and cholinesterases by microplate assays. The antioxidant activity was tested using six in vitro assays. Only the ethanol extract inhibited TYR (80.23 +/- 0.87% at 100 mu gmL(-1)), whereas none of them were able to inhibit cholinesterases. The extracts were more able to scavenge NO radical (31.98 +/- 0.53-56.68 +/- 1.10%) than other radicals tested, and displayed low to moderate activity in the rest of the assays. HPLC analysis revealed that the aqueous extract of VO contained a substantial amount of vitexin (18.81 +/- 0.047mgg(-1) extract), while the ethanol extract also possessed rutin (1.31 +/- 0.013mgg(-1) extract) and vitexin (4.65 +/- 0.103mgg(-1) extract). Furthermore, three flavonoids (rutin, isovitexin, and kaempferol-6-glucoside) were isolated from the ethanol extract. This is the first report on TYR inhibitory activity of VO as well as presence of vitexin and isovitexin in this species. Copyright (c) 2015 John Wiley & Sons, Ltd.
  • PublicationMetadata only
    Antinociceptive Effects of Turkish Endemic Eryngium kotschyi Boiss. Roots by Bioactivity Guided Fractionation
    (2016-01-01T00:00:00Z) Erdem, Sinem Aslan; ARIHAN, OKAN; MITAINE-OFFER, Anne-Claire; İSKİT, ALPER BEKTAŞ; Kartal, MURAT; LACAILLE-DUBOIS, Marie-Aleth; KARTAL, MURAT
    Eryngium species (Apiaceae) are well known plants in ethnobotanical culture throughout world and also in Turkey. They are used as antitussive, diuretic as well as for analgesic and antiinflammatory purposes in traditional medicine. This study aimed to evaluate the antinociceptive activity of endemic Eryngium kotschyi Boiss. root extracts by bioguided fractionation. The antinociceptive activity of the extracts/fractions/compound was studied in mice using acetic acid induced writhing test and and hot plate test. The methanolic extract was sequentially partitioned with hexane, dichloromethane and water saturated n-butanol. Among the fractions, the n-BuOH fraction showed the most significant results in both hot plate test (n-butanol 18,83 +/- 1.81 s p<0,05 versus control: 8.33 +/- 0.67 s) and acetic acid induced writhing test (n-butanol 19,17 +/- 2,41 p < 0,005 versus control 32,67 +/- 2,23) and was chosen for further bioguided purifications by column chromatography and MPLC yielding a pure known triterpene saponin which was characterized as a derivative of A1-barrigenol, showing a moderate antinociceptive activity by hot-plate test (14,33 +/- 0,33 s, P < 0.05 versus control). These findings contribute to the justification of the ethnobotanical use and relevance of Eryngium species. The results suggest there may be a synergy of compounds in the selected activity and support the usage of bioguided fractionation in the search for responsible compounds in plants for antinociceptive activity. Further studies are needed to elucidate the mechanism for action and the structure of compounds which might be responsible of the effect.
  • PublicationMetadata only
    Sage tea-thyme-peppermint hydrosol oral rinse reduces chemotherapy-induced oral mucositis: A randomized controlled pilot study
    (2016-08-01T00:00:00Z) Yayla, Ezgi Mutluay; İZGÜ, NUR; ÖZDEMİR, LEYLA; Erdem, Sinem Aslan; Kartal, MURAT; KARTAL, MURAT
    Objective: This pilot study aimed to investigate the preventive effect of sage tea-thyme-peppermint hydrosol oral rinse used in conjunction with basic oral care on chemotherapy-induced oral mucositis.
  • PublicationMetadata only
    Evaluation of Cholinesterase Inhibitory and Antioxidant Activities of Wild and Cultivated Samples of Sage (Salvia fruticosa) by Activity-Guided Fractionation
    (2011-11-01T00:00:00Z) Senol, Fatma Sezer; Orhan, Ilkay Erdogan; Erdem, Sinem Aslan; Kartal, Murat; Sener, Bilge; Kan, Yuksel; Celep, Ferhat; Kahraman, Ahmet; DOĞAN, MUSA; KARTAL, MURAT
    In European folk medicine, Salvia species have traditionally been used to enhance memory. In our previous study of 55 Salvia taxa, we explored significant anticholinesterase activity of cultivated S. fruticosa. In this study, we compared the inhibitory activity of dichloromethane, ethyl acetate, and ethanol extracts of 3 wild-grown samples and 1 cultivated sample of S. fruticosa against acetylcholinesterase and butyrylcholinesterase enzymes (which are associated with pathogenesis of Alzheimer-s disease) by using the spectrophotometric Ellman method. Antioxidant activities were assessed by determining 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, iron-chelating capacity, and ferric-reducing antioxidant power. The dichloromethane extract of the cultivated sample was then subjected to fractionation by using open column chromatography and medium-pressure liquid chromatography to obtain the most active fraction by activity-guided fractionation. All fractions and subfractions were tested in the same manner, and inactive subfractions were discarded. The essential oil of the cultivated sample was analyzed by gas chromatography-mass spectrometry.