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SELEK, ŞAHABETTİN

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ŞAHABETTİN
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SELEK
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Now showing 1 - 10 of 17
  • PublicationOpen Access
    Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis
    (2018-01-01) Koyuncu, Ismail; Kocyigit, ABDÜRRAHİM; Ozer, Ahmet; SELEK, Sahabettin; Kirmit, Adnan; Karsen, Hasan; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN
    Serologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humans
  • PublicationMetadata only
    Investigation of Oxidative Stress Markers in the Pathogenesis of the Lung, Breast and Colon Cancer
    (2017-09-23T00:00:00Z) ÖZER, ÖMER FARUK; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHBETTİN; ÇOBAN, GANİME; TÜRK, HACI MEHMET; ÖZER, ÖMER FARUK; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN; ÇOBAN, GANİME; TÜRK, HACI MEHMET
  • PublicationMetadata only
    Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters
    (2012-09-01T00:00:00Z) Erturk, Cemil; Altay, Mehmet Akif; Selek, Sahbettin; Kocyigit, ABDÜRRAHİM; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİM
    Background. The aim of this study was to investigate serum paraoxonase-1 (PON1) activity and oxidative/anti-oxidative status in knee osteoarthritis (OA), and evaluate their relationship using radiological and clinical parameters. Materials and methods. The study population comprised 127 patients with knee OA and 107 healthy volunteers. Patients with knee OA were divided into four subgroups according to the Kellgren-Lawrence (K&L) grading scale. In addition, each patient was clinically evaluated by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Serum PON1 activity was measured spectrophotometrically. Oxidative status was assessed by measuring serum lipid hydroperoxide (LOOH) and total oxidant status (TOS). Anti-oxidative status was assessed by measuring serum free sulfydryl groups (-SH = total thiol) and total antioxidant capacity (TAC). Oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods. Results. Serum PON1 activity was significantly lower in the knee OA group compared to the control group (p 0.05). The lowest and highest mean serum PON1 activities were detected in patients with grades 4 and 1, respectively (ANOVA p < 0.001). In multiple regression analysis, WOMAC score was independently associated with serum PON1 activity (beta = -0.248, p = 0.027). Conclusions. Decreased serum PON1 activity and elevated LOOH, TOS, and OSI levels may be associated with knee OA, and serum PON1 activity may be a useful adjunctive indicator of the severity of knee OA for follow-up.
  • PublicationMetadata only
    Mononuclear leukocyte DNA damage and oxidative stress: The association with smoking of hand-rolled and filter-cigarettes
    (2011-04-03T00:00:00Z) Kocyigit, ABDÜRRAHİM; Selek, ŞAHBETTİN; Celik, Hakim; Dikilitas, Murat; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN
    Cigarette smoking is a major cause of human cancer at various sites, although its carcinogenic mechanisms still remain unestablished. Based on the use of a filter, cigarette smoke can be divided into a gas phase and a tar phase. Both contain different concentrations of oxidants, free radicals and tobacco-specific carcinogens. To explore the effects of both filtered and non-filtered cigarette smoke on DNA damage and oxidative status, we measured the level of mononuclear leukocyte DNA damage by use of the single-cell gel electrophoresis (Comet) assay. We also determined malondialdehyde (MDA), protein carbonyl content (PC) and total antioxidative capacity (TAC) levels in blood plasma of smokers of manufactured filter-cigarettes and of hand-rolled cigarettes. Cotinine levels were also measured in plasma to estimate the degree of smoking. Mononuclear leukocyte DNA damage, plasma MDA, plasma PC and plasma cotinine levels were found significantly higher, while plasma TAC levels were found significantly lower in smokers of filter-cigarettes and smokers of hand-rolled cigarettes, compared with control subjects. TAC levels in hand-rolled and manufactured filter-cigarette smokers were not significantly different from each other. However, the levels of DNA damage. plasma MDA, plasma cotinine, and plasma protein oxidation were significantly higher in hand-rolled cigarette smokers than in filter-cigarette smokers. There was a significant positive correlation between MDA and DNA damage in both hand-rolled cigarette smokers and manufactured filter-cigarette smokers. This study indicates that smoking of hand-rolled cigarettes has stronger genotoxic and oxidative effects on the metabolism than smoking of manufactured filter-cigarettes. We propose that these harmful effects could be attributed to the higher level of oxidants. (C) 2011 Elsevier B.V. All rights reserved.
  • PublicationOpen Access
    Paraoxonase-1 Phenotype and Its Relationship with Mean Platelet Volume and Oxidative Stress in Coronary Artery Disease
    (2015-09-01) SELEK, ŞAHBETTİN ; ÖZER, ÖMER FARUK ; GOKTEKİN, Omer ; KOÇYİĞİT, ABDÜRRAHİM; KALINBACOGLU, Ceren; ISLEK, Irem ; ISLEK, Tuğba; ARPACI, Beyza; Erol, Neval; MEYDAN, Sedat; GÜLER, ERAY METİN; SELEK, ŞAHABETTİN; ÖZER, ÖMER FARUK; KOÇYİĞİT, ABDÜRRAHİM; MEYDAN, SEDAT; GÜLER, ERAY METİN
    Objective: Paraoxonase-1 (PON1) 192 QR polymorphism is believed to be an important protective factor for coronary artery disease (CAD); oxidative stress plays a key role in the development of atherosclerotic CAD. Mean platelet volume (MPV) is also central to the processes, including pathophysiology of CAD and endothelial dysfunction. Thus, we aimed to determine the PON1 phenotype, MPV, and oxidative stress parameters in patients with angiographically proven CAD and to compare them with those in healthy subjects. Methods: Fifty-five CAD patients were diagnosed according to the angiography results, and 37 healthy subjects were present in this study. Serum paraoxonase and arylesterase activities were spectrophotometrically measured. Phenotype distribution was evaluated by the salt-stimulated paraoxonase activity according to arylesterase activity. Oxidative stress markers were evaluated by measuring serum total oxidant status (TOS) and total antioxidant status (TAS) as well as oxidative stress index. Results: In this study, the ratio of salt-stimulated paraoxonase/ OSI levels (S-PON1/OSI) were lower in the CAD patients and the differences were statistically significant (p<0.05). Therefore, the ratio of salt-stimulated paraoxonase/MPV (S -PON1/MPV) and S- PON1/OSI level were significantly different in the CAD patients as compared with controls group (p<0.01). Conclusion: Our study has suggested that S-PON1/OSI and SPON1/ MPV may play a significant role in CAD. To the best of our knowledge, the present study is the first to study the relationship among PON1 phenotype, MPV, and OSI in CAD patients. Thus, lowering of the oxidative stress and the regulation of MPV strategies may be a promising approach for the treatment of CAD.
  • PublicationMetadata only
    Raftlin, presepsin levels and thiol-disulfide homeostasis in acute appendicitis: A pilot study
    (2018-11-01) KOÇYİĞİT, ABDÜRRAHİM; ERSOY, YELİZ EMİNE; SELEK, ŞAHBETTİN; SÜMBÜL, BİLGE; ÖZER, ÖMER FARUK; GÜLER, ERAY METİN; MERAL, İSMAİL; YİĞİT, MEHMET; ÖZER, ÖMER FARUK; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; MERAL, İSMAİL; SÜMBÜL, BİLGE; SELEK, ŞAHABETTİN; ERSOY, YELIZ EMINE
    Objective: To investigate some of the new inflammatory and oxidative stress markers in acute appendicitis.
  • PublicationOpen Access
    Phyotherapeutic Properties of Urfa Pistachio Nuts (Pistacia vera L.)
    (2018-07-01) Koyuncu, Ismail; KOÇYİĞİT, ABDÜRRAHİM; Dikme, Resat; SELEK, ŞAHBETTİN; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN
    According to recent studies, the Urfa pistachio nut (Pistacia vera L.) is a rich source of phenolic components, with a high level of the three antioxidant substances of gallic acid, catechin, and epicatechin present together. The pistachio nut, which is in the top 50 foodstuffs with the highest antioxidant potential, is the only edible nut containing anthocyanin, which is the richest and has most variations of water and fat-soluble antioxidants. The pistachio nut also contains important bioactive polyphenol components, such as transreservatrol and isoflavones, which have anti-cancer potential. Some forms of the Pistachio species, including the Urfa pistachio nut, are used by the general population to improve the gastrointestinal, liver, urinary tract, and respiratory tract disorders and for various other purposes such as aphrodisiac, antiseptic, and antihypertensive effects. In some studies, it has been determined that the pistachio nut is beneficial in the continuation of mechanisms that provide maintenance of body health, such as anti-inflammatory activity, glycemic control, and endothelial functions, and it has also been shown to prevent the oxidation of low-density lipoprotein cholesterol and to have a protective role against chronic diseases such as cancer and cardiovascular disease. With a potassium content of 10.05 mg/gram, which is higher than other types of peanut, the sodium content of the pistachio nut may play a role in balancing the blood pressure by reducing the need for salt. This review is of importance in respect of the evaluation of the positive effect on several diseases of the kernel and particularly the shell extract of the pistachio nut.
  • PublicationMetadata only
    Peripheral DNA damage in active pulmonary tuberculosis
    (2012-05-01T00:00:00Z) Selek, Sahbettin; ASLAN, Mehmet; Horoz, Mehmet; Celik, Hakim; Cosar, Niyet; Gunak, Faruk; Kocyigit, ABDÜRRAHİM; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİM
    In pulmonary tuberculosis patients, little is known about peripheral DNA damage, although increased oxidative stress is a well documented entity. Therefore, we aimed to investigate DNA damage along with oxidative status parameters in pulmonary tuberculosis patients. Twenty-seven pulmonary tuberculosis patients and 26 controls were included. DNA damage was assessed by comet assay. Total oxidant and antioxidant status, and oxidative stress index were determined. DNA damage, total oxidant status and oxidative stress index were higher in pulmonary tuberculosis patients than controls (all P < 0.05), while total antioxidant status was lower (P < 0.05). DNA damage was correlated with total oxidant and antioxidant status, and oxidative stress index (r = 0.69, P < 0.05; r = 0.48, P < 0.05, r = -0.47, P < 0.05; respectively) in pulmonary tuberculosis patients. Oxidative stress and DNA damage are increased in pulmonary tuberculosis patients. Increased oxidative stress associated DNA damage may be one of the pathogenetic mechanisms involved in the disorders suggested to be associated with pulmonary tuberculosis. (C) 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012.
  • PublicationMetadata only
    Raftlin, Presepsin Levels and Thiol-Disulfide Homeostasis in Acute Appendicitis
    (2017-09-23) Özer, Ömer Faruk; Güler, Eray Metin; Koçyiğit, Abdürrahim; Selek, Şahbettin; Gultepe, Bs; Yigit, Mehmet; Ersoy, Ye; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN
  • PublicationMetadata only
    Protective effect of beta-glucan on renal ischemia and reperfusion injury in young and aged rats.
    (2014-09-12) EŞREFOĞLU, MUKADDES; TOK, OLGU ENİS; AYDIN, M. S.; IRAZ, M.; SELEK, ŞAHBETTİN; ÖZER, ÖMER FARUK; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDES; TOK, OLGU ENİS; SELEK, ŞAHABETTİN; ÖZER, ÖMER FARUK; KOÇYİĞİT, ABDÜRRAHİM