Person: KUMAŞ, MELTEM
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Publication Metadata only Protective effects of silymarin against kidney injury caused by high dose administration of isotretinoin in mice.(2015-09-24) Kumaş, MELTEM; EŞREFOĞLU, MUKADDES; Kesgin, S; Özer, OF; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; ÖZER, ÖMER FARUKPublication Metadata only Diyabetik sıçanlarda malign serebral iskemi ve iskemik ön koşullanmanın testis dokusu üzerine uzak doku etkilerinin histopatolojik açıdan incelenmesi(2015-12-03) KUMAŞ, MELTEM; ALTINTAS, OZGE; EŞREFOĞLU, MUKADDES; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDESPublication Metadata only Relationship between regular exercise-induced cardiac hypertrophy and micro RNA(2017-09-30) PALA, MUKADDES; ALTAN, MEHMET; SÖNMEZ, OSMAN FUAT; MENGİ, MURAT; Dinçer, Şensu; AKBAŞ, FAHRİ; YILDIZ, MUSTAFA; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; METİN, GÖKHAN; AKBAŞ, FAHRİ; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDESPublication Open Access Protective Effects of Curcumin on Cadmium-Induced Renal Injury in Young and Aged Rats(2016-12-01) Kumas, MELTEM; Esrefoglu, MUKADDES; Bayindir, NİHAN; Iraz, Meryem; Ayhan, Siddika; Meydan, SEDAT; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; BAYINDIR, NİHAN; MEYDAN, SEDATObjective: We aimed to investigate the protective effects of curcumin (Cr) against cadmium (Cd) toxicity on the kidneys of both young and aged rats. Methods: Forty-eight young and aged female Spraque–Dawley rats were divided into control, Cd, Cr, and Cd+Cr groups. We investigated kidney damage using a histopathological scoring system and measured total antioxidant status (TAS) and total oxidant status (TOS) and interleukin-6 (IL-6), procalcitonin (PCT), and C-reactive protein (CRP) levels. Results: Kidney tissues of Cd groups showed acute histopathological alterations. Cr improved Cd-induced histopathological changes (p<0.05). The highest mean TAS was recorded in both the Cr groups. The highest mean TOS was recorded only in the aged Cd group. Cr decreased IL-6 levels in both the Cd+Cr groups (p<0.05). PCT levels in the Cd groups were higher than those in the control groups. Significance was detected only between the young Cd and control groups (p<0.05). PCT levels were reduced in both the Cd+Cr groups (p<0.05). CRP levels in the aged Cd group were higher than those in the other groups (p<0.05). Cr reduced CRP levels only in the aged Cd+Cr group (p<0.05). Conclusion: Our results suggest that Cr prevents Cd-induced renal oxidative damage in both young and aged rats.Publication Open Access Investigation of dose-dependent effects of berberine against renal ischemia/reperfusion injury in experimental diabetic rats(2019-01-01) Kanbay, Songül; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; Karataş, Ersin; Duymaç, Nurcihan; ERGÜN, İLYAS SAMET; ÜYÜKLÜ, MEHMET; KOÇYİĞİT, ABDÜRRAHİM; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; ERGÜN, İLYAS SAMET; ÜYÜKLÜ, MEHMET; KOÇYİĞİT, ABDÜRRAHİMBackground: Ischemia-reperfusion injury causes various severe morphological and functional changes in diabetic patients. To date, numerous antidiabetic and antioxidant agents have been used for treatment of the disease-related changes. Objectives: We aimed to examine effective therapeutic doses or doses of berberine against renal ischemia/reperfusion injury (IRI) in a streptozotocin (STZ)-induced diabetic rat model by histopathological and biochemical analysis. Methods: Thirty male Sprague Dawley rats were treated with STZ injection for the development of diabetes, and divided into the following groups: STZ-induced diabetic group (STZ); IRI-induced diabetic group (STZ+IRI); 50mg/kg berberine (BRB) treated diabetic group after inducing IRI (STZ+IRI+BRB1); 100mg/kg BRB treated diabetic group after IRI (STZ+IRI+BRB2); 150mg/kg BRB treated diabetic group after IRI (STZ+IRI+BRB3). Bilateral renal ischemia model was applied for 45min, then reperfusion was allowed for 14 days in STZ-induced diabetic rats. Renal injury was detected histopathologically. Blood urea nitrogen (BUN), creatinine and lactate dehydrogenase (LDH) levels were measured in serum using the ELISA method. Total antioxidant status (TAS) and total oxidant status (TOS) of renal tissue was studied by spectrophotometric assay. Oxidative stress index (OSI) was calculated as TOS-to-TAS ratio. Tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP), Na+/K+-ATPase (sodium pump), and Ca2+-ATPase (calcium ATPase) enzyme levels were measured in tissues using the ELISA method. Anti-apoptotic Bax and pro-apoptotic Bcl-2 protein levels were detected by Western blot analysis. All data were evaluated statistically. Results: The highest histopathological score was detected in the STZ+IRI group compared to the other group. BRB administration at the doses of 100mg/kg and 150mg/kg markedly improved renal injury. BUN and creatinine levels significantly increased in the STZ+IRI group compared to the STZ group (p<0.001). 100mg/kg and 150mg/kg BRB administration significantly decreased those levels (p<0.01). The highest TOS and the lowest TAS levels were detected in the STZ+IRI group (p<0.001). IRI markedly aggravated inflammation via increasing levels of TNF-α and CRP (<0.001), and caused apoptosis via inducing Bcl-2 protein, and suppressing Bax protein (p<0.001). BRB administration at the doses of 100mg/kg and 150mg/kg showed anti-oxidant, anti-inflammatory and anti-apoptotic effects (p<0.01). The LDH enzyme, was used as a necrosis marker, was higher in the STZ+IRI group than other groups. BRB administration at all of the doses, resulted in the decline of LDH enzyme level (p<0.001). Ca2+-ATPase and Na+/K+-ATPase enzyme activities decreased in the STZ+IRI group compared to the STZ group (p<0.001), while BRB administration at the doses of 100mg/kg and 150mg/kg significantly increased those of enzyme activities, respectively (p<0.05). Conclusion: Ischemia with diabetes caused severe histopathological and biochemical damage in renal tissue. The high doses of berberine markedly improved histopathological findings, regulated kidney function via decreasing BUN and creatinine levels, and rearranged intercellular ion concentration via increasing Na+/K+-ATPase and Ca2+- ATPase levels. Berberine showed anti-oxidant, anti-apoptotic, and anti-inflammatory effects. According to these data, we suggest that berberine at the doses of 100 and 150mg may be used as a potential therapeutic agent to prevent renal ischemic injury.Publication Metadata only Relationship between regular exercise-induced cardiac hypertrophy and micro RNA(2017-09-01) Pala, M.; Altan, M.; Sonmez, O. F.; Mengi, M.; Dincer, S.; AKBAS, FAHRİ; Yildiz, M.; KUMAS, MELTEM; Esrefoglu, MUKADDES; Metin, G.; AKBAŞ, FAHRİ; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDESPublication Metadata only Ratlarda Kurkuminin Kadmiyum ile İndüklenen Karaciğer Apopitozu Üzerine Koruyucu Etkisi(2016-12-01) BAYINDIR, NİHAN; EŞREFOĞLU, MUKADDES; KUMAŞ, MELTEM; Iraz, Meryem; Ayhan, Sıddıka; Kılıç, Elif; BAYINDIR, NİHAN; EŞREFOĞLU, MUKADDES; KUMAŞ, MELTEMPublication Metadata only Farelerde yüksek doz isotretinoin uygulamasının yol açtığı kalp dokusu hasarına karşı silymarinin olası etkilerinin araştırılması.(2016-06-01) KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; Özer , ÖMER FARUK; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; ÖZER, ÖMER FARUKPublication Metadata only Protective effects of silymarin against isotretinoin induced liver and kidney injury in mice(2018-03-01) Kumas, MELTEM; Esrefoglu, MUKADDES; Guler, ERAY METİN; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES; GÜLER, ERAY METİNIsotretinoin (ISR), the common therapeuticagent for acne vulgaris, when used long term, leads to various side effects viz., oxidative toxicity, renal and hepatic dysfunction, depression, congenital abnormalities, aortic art defects, microcephaly, etc. Here, we explored the effects of silymarin (SLY), a flavonolignan from the seeds of the milk thistle Silybum marianum (L.) which has potential to protect the liver against chemical and environmental toxins and increase proliferation rate of tubule cells, against ISR induced liver and kidney injury. Thirty-two male Balb/c mice (3 months of age) were divided into four groups: control, isotretinoin (ISR, 40 mg/kg/day), silymarin (SLY, 200 mg/kg/day), and ISR+SLY group. We investigated liver and kidney injury by histopathological scoring system, and apoptotic cells labelled by TUNEL method. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured in serum samples biochemically. ALT and AST levels were increased in ISR group (P = 0.025, P = 0.003, respectively). SLY decreased those levels in ISR+SLY group (P = 0.002, P = 0.013, respectively). Liver tissues of ISR group showed interstitial edema and necrosis, alteration in shape and size of nuclei, mononuclear and kuppfer cell infiltration. Kidney tissues of ISR group showed tubular degeneration, necrosis, glomerular collapse, mononuclear cell infiltration, and hemorrhage. SLY improved those histopathological changes and suppressed apoptotic cell death. We suggest that silymarin might be beneficial to some extent by preventing the side effects induced by chronic ISR therapy in patients with acne vulgaris.Publication Metadata only Protective effect of ıschemic preconditioning on myocardium as remote tissue ınjury following transient focal cerebral ıschemia in diabetic and non-diabetic rats.(2016-05-12) KUMAŞ, MELTEM; altıntaş, özge; EŞREFOĞLU, MUKADDES; KUMAŞ, MELTEM; EŞREFOĞLU, MUKADDES