Person: EROL, EBRU
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Publication Metadata only Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii - A forgotten endemic angiosperm of Turkey(2016-09-01T00:00:00Z) Yildiztekin, Fatma; Nadeem, Said; Erol, EBRU; Yildiztekin, Mahmut; Tuna, Atilla L.; Ozturk, Mehmet; EROL, EBRUContext We report the first ever chemical/biochemical study on Crocus mathewii Kerndorff (Iridaceae) - a Turkish endemic angiosperm. This plant has never been explored for its phytochemistry and bioactivities.Objective This study explores C. mathewii corm and aerial parts for the chemical and biological properties of hexane, ethyl acetate, methanol and water fractions of the extracts.Material and methods Plant material (20g) was extracted by methanol (250mLx5, 3 days each) and fractioned into hexane, ethyl acetate, methanol and water. All fractions were subjected to -carotene-linoleic acid, DPPH, ABTS(+), CUPRAC, metal chelating and tyrosinase inhibition activities. Hexane fractions were submitted to GC-MS analysis.Results Ethyl acetate fractions showed excellent IC50 values in DPPH (aerial 36.210.76 and corm 33.87 +/- 0.02mg/L) and ABTS(+) (aerial 33.01 +/- 0.79 and bulb 27.87 +/- 0.33mg/L); higher than the IC50 of the standard -tocopherol (DPPH 116.25 +/- 1.97; ABTS 52.64 +/- 0.37mg/L), higher than BHA in DPPH (57.31 +/- 0.25mg/L), but slightly lower in ABTS (19.86 +/- 2.73mg/L). Methanol extract of aerial parts also showed higher activity than -tocopherol in DPPH (85.56 +/- 11.51mg/L) but slightly less (72.90 +/- 3.66mg/L) than both the standards in ABTS. Linoleic (aerial 53.9%, corm 43.9%) and palmitic (aerial 22.2%, corm 18%) were found as the major fatty acids.Discussion and conclusion Some fractions of C. mathewii showed higher antioxidant activities than the standards. There is a need to explore more about this plant.Publication Metadata only Chemical constituents of essential oil of endemic Rhanterium suaveolens Desf. growing in Algerian Sahara with antibiofilm, antioxidant and anticholinesterase activities(2016-01-01T00:00:00Z) Chemsa, Ahmed Elkhalifa; Erol, EBRU; Ozturk, Mehmet; Zellagui, Amar; Ozgur, Ceylan; Gherraf, Noureddine; Duru, Mehmet Emin; EROL, EBRUTwenty compounds were detected in the essential oil of Rhanterium suaveolens representing 98.01% of the total oil content. Perillaldehyde (45.79%), caryophyllene oxide (24.82%) and -cadinol (5.61%) were identified as the main constituents. In -carotene-linoleic acid assay, both the oil and the methanol extract exhibited good lipid peroxidation inhibition activity, with IC50 values of 17.97 +/- 5.40 and 11.55 +/- 3.39g/mL, respectively. In DPPH and CUPRAC assays, however, the methanol extract exhibited a good antioxidant activity. The highest antibiofilm activity has been found 50.30% against Staphylococcus epidermidis (MU 30) at 20g/mL for essential oil and 58.34% against Micrococcus luteus (NRRL B-4375) at 25mg/mL concentration for methanol extract. The in vitro anticholinesterase activity of methanol extract showed a moderate acetylcholinesterase inhibitory (IC50=168.76 +/- 0.62g/mL) and good butyrylcholinesterase inhibitory (IC50=54.79 +/- 1.89g/mL) activities. The essential oil was inactive against both enzymes.Publication Metadata only Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of essential oil and methanolic extract of Anthemis stiparum subsp sabulicola (Pomel) Oberpr(2018-06-01T00:00:00Z) Chemsa, Ahmed Elkhalifa; Zellagui, Amar; Ozturk, Mehmet; Erol, EBRU; Ceylan, Ozgur; Duru, Mehmet Emin; Lahouel, Mesbah; EROL, EBRUAnthemis species are traditionally used to treat infectious and inflammatory processes, among others clinical disturbances. In the current study, the chemical composition, the total phenolic and flavonoid contents, the antioxidant, anticholinesterase, antimicrobial, and antibiofilm activities of Anthemis stiparum subsp. sabulicola aerial parts methanolic extract (As-ME) and essential oil (As-EO) were investigated. The chemical composition of As-EO was established by GC-MS and GC-FID. Total phenolic and flavonoid contents of As-ME were spectrophotometrically determined. Diphenyl-1-picrylhydrazyl (DPPH center dot) radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and beta-carotene bleaching assays were applied to evaluate the antioxidant potential. The anticholinesterase activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were carried out spectrophotometrically. The antimicrobial activity was assessed by Minimal Inhibitory Concentration (MIC) using broth microdilution method against 7 ATCC(center dot) bacterial and one ATCC(center dot) yeast reference strains. The antibiofilm effect was determined quantifying the percentage of adhesion inhibition. GC-MS and GC-FID identified 72 compounds (99.02%), being As-EO predominantly constituted by germacrene D (11.13%), t-cadinol (11.01%), camphor (6.73%), spathulenol (6.50%) and isoamyl salicylate (6.45%). The total phenolic and flavonoid contents of As-ME were 13.6 +/- 0.03 and 5.9 +/- 0.04 pyrocatechol equivalents and quercetin equivalents, respectively. In beta-carotene-linoleic acid assay, As-ME showed the best lipid peroxidation inhibition activity with an IC50 = 9.96 mu g/mL followed by As-EO with an IC50 = 619.98 mu g/mL. In contrast, in DPPH assay, As-ME and As-EO showed moderate to low activity with an IC50 = 92.69 mu g/mL for As-ME and 917.69 mu g/mL for As-EO. While in CUPRAC assay, As-EO and As-ME indicated a less to moderate reducing activity. As-ME inhibited AChE (IC50 = 490.46 mu g/mL) and BChE (IC50 = 142.07 mu g/mL), while As-EO was inactive against AChE and revealed a discreet inhibitory action against BChE (IC50 = 212.14 mu g/mL). As-ME displayed better antimicrobial activity than As-EO, being active against Staphylococcus aureus (ATCC(center dot) 25923) and Bacillus subtilis (ATCC(center dot) 6633), with MIC of 1.56 mg/mL. An expressive fungal adhesion inhibition (80.02%) on Candida albicans (ATCC(center dot) 10239) was detected with As-ME at 6.25 mg/mL. These results showed that A. stiparum subsp. sabulicola is a natural source of active compounds with antibiotic and antibiofilm effects against S. aureus and B. subtilis, and C. albicans, respectively, and also presents antioxidant and anticholinesterase properties.Publication Metadata only Berberis Plants-Drifting from Farm to Food Applications, Phytotherapy, and Phytopharmacology(2019-10-01T00:00:00Z) Salehi, Bahare; Selamoglu, Zeliha; Sener, Bilge; Kilic, Mehtap; Jugran, Arun Kumar; de Tommasi, Nunziatina; Sinisgalli, Chiara; Milella, Luigi; Rajkovic, Jovana; Morais-Braga, Maria Flaviana B.; Bezerra, Camila F.; Rocha, Janaina E.; Coutinho, Henrique D. M.; Ademiluyi, Adedayo Oluwaseun; Shinwari, Zabta Khan; Jan, Sohail Ahmad; Erol, EBRU; Ali, Zulfiqar; Ostrander, Elise Adrian; Sharifi-Rad, Javad; de la Luz Cadiz-Gurrea, Maria; Taheri, Yasaman; Martorell, Miguel; Segura-Carretero, Antonio; Cho, William C.; EROL, EBRUThe genus Berberis includes about 500 different species and commonly grown in Europe, the United States, South Asia, and some northern areas of Iran and Pakistan. Leaves and fruits can be prepared as food flavorings, juices, and teas. Phytochemical analysis of these species has reported alkaloids, tannins, phenolic compounds and oleanolic acid, among others. Moreover, p-cymene, limonene and ocimene as major compounds in essential oils were found by gas chromatography. Berberis is an important group of the plants having enormous potential in the food and pharmaceutical industry, since they possess several properties, including antioxidant, antimicrobial, anticancer activities. Here we would like to review the biological properties of the phytoconstituents of this genus. We emphasize the cultivation control in order to obtain the main bioactive compounds, the antioxidant and antimicrobial properties in order to apply them for food preservation and for treating several diseases, such as cancer, diabetes or Alzheimer. However, further study is needed to confirm the biological efficacy as well as, the toxicity.Publication Metadata only Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC-ESI-MS/MS with chemometric approach(2021-01-01T00:00:00Z) Mokaddem-Daroui, Habiba; Mostefa, Maya Belhadj; Aydogmus-Ozturk, Fatma; Erol, EBRU; Ozturk, Mehmet; Ertas, Abdulselam; Duru, Mehmet Emin; Kabouche, Ahmed; Kabouche, Zahia; EROL, EBRUThe fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The beta-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 mu g/mL), b-carotene-linoleic acid test (IC50: 18.6 mu g/mL) and CUPRAC (A(0.5): 33.8 mu g/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 mu g analyte/g extract), hyperoside (353.0-2045.5 mu g/g), vanillin (1488.9-1529.9 mu g/g), tannic acid (1281.6-1416.8 mu g/g), rosmarinic acid (1063.1-1149.3 mu g/g), coumarin (683.0-772.6 mu g/g), quercetin (881.0 mu g/g), and catechin (277.7-400.2 mu g/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially.Publication Metadata only Inhibition of iNOS Induction and NF-kappa B Activation by Taste Compounds from the Edible Mushroom Tricholoma caligatum (Viv.) Ricken(2020-01-01T00:00:00Z) Erol, EBRU; Ali, Zulfiqar; Ozturk, Mehmet; Khan, Shabana; Khan, Ikhlas A.; EROL, EBRUTricholoma caligatum (Viv.) Ricken is an edible mushroom that belongs to matsutake group. The first chemical investigation of the three different extracts of Tricholoma caligatum resulted in two new compounds, 8-demethoxylascivol (1) and 8-epi-lascivol (2) and six known compounds, lascivol (3), trametenolic acid (4), ergosterol (5), ergosterol peroxide (6), 5 alpha, 6 alpha-epoxyergosterol (7), and cerebroside B (8). Their structures were elucidated by spectroscopic analyses including 1D and 2D NMR data. The biological activities of all the compounds were evaluated toward multiple targets related to inflammation and metabolic disorder such as NF-kappa B, iNOS and ROS. The findings of this study reveal that the edible mushroom Tricholoma caligatum could be a potential source for anti-inflammatory bioactive metabolites.Publication Metadata only Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica(2017-01-01T00:00:00Z) Ferhat, Maria; Erol, EBRU; Beladjila, Khadidja Aya; Cetintas, Yunus; Duru, Mehmet Emin; Ozturk, Mehmet; Kabouche, Ahmed; Kabouche, Zahia; EROL, EBRUContext: Stachys guyoniana Noe ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine.