Person: TOPÇU, GÜLAÇTI
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Publication Metadata only Investigation of Anticholinesterase Activity of a Series of Salvia Extractsand the Constituents of Salvia staminea(2013-03-01) Topçu, Gülaçtı; Boğa, Mehmet; Kolak, Ufuk; Öztürk, Mehmet; Dirmenci, Tuncay; Bahadori, Fatemeh; Bütün, Burcu; TOPÇU, GÜLAÇTI; BAHADORİ, FATEMEH; BÜTÜN, BURCUPublication Metadata only Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation(2011-05-01T00:00:00Z) Ozturk, Mehmet; Kolak, Ufuk; Topcu, GÜLAÇTI; Oksuz, Sevil; CHOUDHARY, M. Iqbal; TOPÇU, GÜLAÇTIFrom the acetone extract of Micromeria cilicica, two new (1 and 2) and five known (3-7) compounds were obtained through radical-scavenging activity guided isolation. Structures of the compounds were identified as piperitone 7-O-beta-D-glucoside (1), isothymonin 4--methyl ether (2), sudachitin (3), isomucronulatol (4), rutin (5), ursolic acid (6) and saccharose (7), based on UV, 1D-and 2D-NMR and mass spectroscopic techniques. The antioxidant potentials of the extract and the isolated compounds were established by using three radical-scavenging assays, namely, DPPH. scavenging, O-2(center dot-) scavenging and ABTS(center dot+) scavenging, besides beta-carotene bleaching assay. Particularly, the acetone extract showed a strong inhibition of lipid peroxidation by beta-carotene bleaching assay, with a result close to that of (+)-catechin. Among the pure compounds, rutin (5) showed the strongest lipid peroxidation inhibition and antiradical activity while both sudachitin (3) and isomucronulatol (4) exhibited noticeable ABTS(center dot+) scavenging activity. The anticholinesterase activity of the compounds (1-7) was also determined. Against acetylcholinesterase, they exhibited weak inhibition while compounds 3, 4 and 6 exhibited moderate inhibition against butyrylcholinesterase. (C) 2010 Elsevier Ltd. All rights reserved.Publication Metadata only Antioxidant, anticholinesterase, and antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm.(2014-01-01) Ertas, Abdulselam; Boga, Mehmet; Hasimi, Nesrin; Yesil, Yeter; GOREN, AHMET CEYHAN; Topcu, GÜLAÇTI; Kolak, Ufuk; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTIThis study was the first fatty acid analysis and biological activity report on an endemic Achillea species, Achillea cappadocica Hausskn. et Bornm. The major compounds in the fatty acid were identified as oleic (34.7%), palmitic (23.1%), and linoleic acids (20.6%). The methanol and water extracts indicated higher ABTS cation radical scavenging activity than that of alpha-tocopherol and BHT at 100 mu g/mL. The acetone extract showed 70.62% inhibitory activity against butyrylcholinesterase at 200 mu g/mL. The acetone and methanol extracts exhibited moderate antimicrobial activity.Publication Metadata only A potential therapeutic role in multiple sclerosis for stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata(2017-07-01) Acar, Ozden Ozgun; GAZİOĞLU, IŞIL; Kolak, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIMultiple sclerosis (MS) is an autoimmune disease of the human central nervous system. It is one of the most common neurological disorders around the world and there is still no complete cure for MS. Purification of a terpenoid from Capparis ovata was carried out and its structure was elucidated as stigmast-5,22-dien-3 beta-ol, myristate (3 beta, 22E-stigmasteryl myristate; SDM) by NMR and mass spectral analyses. No information regarding its any health effect is available in the literature. In the present study, we have described its effects on inflammatory factors such as the expression levels of cytokines, chemokines and adhesion molecules as well as apoptosis/infiltration and myelination in SH-SY5Y cells. The expression levels of proinflammatory or inflammatory cytokines and chemokines such as NF-.B1, CCL5, CXCL9, CXCL10 and HIF1A along with T-cell activating cytokines such as IL-6 and TGFB1 were significantly downregulated with SDM treatment. Moreover, the expression levels of the main myelin proteins such as MBP, MAG and PLP that are essential for healthy myelin architecture were significantly up-regulated. The results presented in this study strongly suggest that the SDM offers a unique possibility to be used with autoimmune diseases, including MS due to its activity on the manipulation of cytokines and the promotion of myelin formation.Publication Metadata only Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice(2016-09-15) Ozgun-Acar, Ozden; Celik-Turgut, Gurbet; Gazioglu, IŞIL; Kolak, Ufuk; Ozbal, Seda; Ergur, Bekir U.; Arslan, Sevki; Sen, Alaattin; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTISince ancient times, Capparis species have been widely used in traditional medicine to treat various diseases. Our recent investigations have suggested Capparis ovata-s potential anti-neuroinflammatory application for the treatment of multiple sclerosis (MS). The present study was designed to precisely determine the underlying mechanism of its anti-neuroinflammatory effect in a mouse model of MS. C. ovata water extract (COWE) was prepared using the plant-s fruit, buds, and flower parts (Turkish Patent Institute, PT 2012/04,093). We immunized female C57BL/6 J mice with MOG(35_55)/CFA. COWE was administered at a daily dose of 500 mg/Kg by oral gavage either from the day of immunization (T1) or at disease onset (12) for 21 days. Gene expression analysis was performed using a Mouse Multiple Sclerosis RT2 Profiler PCR Array, and further determinations and validations of the identified genes were performed using qPCR. Whole-genome transcriptome profiling was analyzed using Agilent SurePrint G3 Mouse GE 8X60K microarrays. Immunohistochemical staining was applied to brain sections of the control and treated mice to examine the degree of degeneration. COWE was further fractionated and analyzed phytochemically using the Zivak Tandem Gold Triple Quadrupole LC/MS-MS system. COWE remarkably suppressed the development of EAE in T1, and the disease activity was completely inhibited. In the T2 group, the maximal score was significantly reduced compared with that of the parallel EAE group. The COWE suppression of EAE was associated with a significantly decreased expression of genes that are important in inflammatory signaling, such as TNF alpha, IL6, NF-kappa B, CCL5, CXCL9, and CXCK10. On the other hand, the expression of genes involved in myelination/remyelination was significantly increased. Immunohistochemical analysis further supported these effects, showing that the number of infiltrating immune cells was decreased in the brains of COWE-treated animals. In addition, differential expression profiling of the transcriptome revealed that COWE treatment caused the down regulation of a group of genes involved in the immune response, inflammatory response, antigen processing and presentation, B-cell-mediated immunity and innate immune response. Collectively, these results suggest anti-neuroinflammatory mechanisms by which COWE treatment delayed and suppressed the development of EAE and ameliorated the disease in mice with persistent clinical signs. (C) 2016 Elsevier B.V. All rights reserved.Publication Metadata only Antioxidant Activities of Salvia heldreichiana Boiss. Ex Bentham’s Extracts(2011-09-11) Sındırgılı, Burcu; BÜTÜN, BURCU; Kolak, Ufuk; Başkan, Tuba; TOPÇU, GÜLAÇTI; BÜTÜN, BURCU; TOPÇU, GÜLAÇTIPublication Metadata only Stigmast-5,22-dien-3 beta-ol myristate isolated from Capparis ovata having anti-inflammatory and immunomodulatory actions could be of interest for use in multiple sclerosis(2017-08-30) Sen, Alaattin; Acar, Ozden Ozgun; Gazioglu, IŞIL; Kolak, Ufuk; Topcu, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTIPublication Metadata only Anticholinesterase and Antioxidant Activities of Natural Abietane Diterpenoids with Molecular Docking Studies(2020-01-01T00:00:00Z) TOPÇU, GÜLAÇTI; AKDEMİR, ATİLLA; Kolak, Ufuk; Ozturk, Mehmet; Boga, Mehmet; BAHADORİ, FATEMEH; Cakmar, Seda Damla Hatipoglu; TOPÇU, GÜLAÇTI; AKDEMİR, ATİLLA; BAHADORİ, FATEMEHBackground: Alzheimer-s Disease (AD) is one of the most prevalent causes of dementia in the world, and no drugs available that can provide a complete cure. Cholinergic neurons of the cerebral cortex of AD patients are lost due to increased activity of cholinesterase enzymes.Publication Metadata only Anti-neuroinflammatory effect of butanolic subextract of Capparis ovata water extract used as an alternative and complementary treatment for multiple sclerosis(2014-10-15) Sen, Alaattin; Topcu, GÜLAÇTI; Ozgun, Ozden; Kolak, Ufuk; Hacibekiroglu, Isil; Celik, Gurbet; Arslan, Sevki; TOPÇU, GÜLAÇTIPublication Metadata only Two new indole alkaloids from Vinca herbacea L.(2011-12-01T00:00:00Z) Boga, Mehmet; Kolak, Ufuk; Topcu, GÜLAÇTI; Bahadori, Fatemeh; Kartal, Murat; FARNSWORTH, Norman R.; TOPÇU, GÜLAÇTI; BAHADORİ, FATEMEH; KARTAL, MURATSix indole alkaloids were isolated from the aerial parts of Vinca herbacea Waldst. et Kit. Structures of the isolated compounds were elucidated as two new alkaloids, 10-methoxyraucaffrinoline (1) and 11-methoxyburnamine (2), and four known alkaloids, picrinine (3), burnamine (4), caboxine A (5) and elegantine (6) by intensive 1D-and 2D-NMR spectroscopic techniques. Antioxidant and anticholinesterase activities were investigated for the methanol, alkaloidal and non-alkaloidal extracts of V. herbacea and isolated alkaloids (1-3, 6). The alkaloidal extract was found to be active in the three tested antioxidant assays and exhibited significant lipid peroxidation inhibitory and DPPH free radical scavenging activities. The alkaloidal extract also exhibited high anticholinesterase activity against AChE and BChE enzymes similar to galanthamine. However, none of the tested alkaloids 1-3, and 6 were found to be active in the tested antioxidant and anticholinesterase systems. The alkaloids 1, 2, 4, and 6 were also evaluated for cytotoxic activity against the MCF7 and HT29 cell lines, and showed no activity. (C) 2011 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.