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GÖNCÜ, BEYZA SERVET

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Author: Yücesan, Emrah ×

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    Nano- and Micro-Encapsulation Techniques and Applications
    (2021-01-01T00:00:00Z) Göncü, Beyza Servet; Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH
    Microencapsulation has been the most frequently used technique for several different disciplines such as cell-based therapies and/or transplantation. Technology is based on the idea of combining and coating material or isolating it from an external source. Microencapsulation may be performed with different materials and, among natural biocompatible materials, alginate-based microencapsulation technique is the most appropriate material for microencapsulation. The structural components of alginate materials are the derivatives of alginic acid, which is found in brown algae as an intercellular gel matrix. This alginate is preferred for clinical applications due to its safety in human studies. Therefore, the choice and the combined system need to be carefully optimized to achieve biocompatible application through cell microencapsulation especially for long term. Specifications of alginate such as primary source, isolation process, viscosity, and purity contribute to improve its biocompatibility. Clinically, cell microencapsulation is the major contribution to the field of transplantation by its technique and additionally provides local immune isolation. This chapter discusses the potential benefits of clinically suitable alginates and their applications. This promising technology may highlight its considerable potential for patients that require transplantation and/or replacement therapy in the future.
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    Paratiroit Hücre İzolasyonunda Enzimatik ve Mekanik İzolasyon Yöntemlerinin Karşılaştırılması
    (2018-05-04T00:00:00Z) Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH
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    Identification and functional characterization of a novel homozygous mutation in KCNMA1 encoding voltage and calcium sensitive potassium channel is associated with dyskinesia, epilepsy, intellectual disability, cerebellar and corticospinal tract atrophy
    (2020-12-01T00:00:00Z) Servet Göncü, Beyza; Aslanger, Ayça Dilruba; Özgül, Cemil; Hasanoğlu, Sevde; Yeşil Sayın, Gözde; YÜCESAN, EMRAH; GÖNCÜ, BEYZA SERVET
    Introduction: KCNMA1 encodes, the alpha subunit of the voltage, and calcium-sensitive potassium channel, predominantly expressed in the central nervous system. Therefore abnormal function in this gene may occur neurological conditions.Materials and Methods: We report 15-year-old patient who was born at term with healthy conditions. Motor signals were delayed, and also seizures started at the age of 18 months. EEG revealed generalized spike-wave activities. Brain MRI performed, atrophy of the cerebellum was detected. Recent clinical examination; contractures on the large joints, and dyskinetic tremor. Whole exome sequencing (WES) was performed and in-slico analyses were conducted. MCF7 and 293T cells transfected with either wild-type or mutant expression vectors. Cellular distribution was determined by immunofluorescence. Functional analysis was performed using electrophysiological approach based on whole-cell patch-clamp.Results: WES revealed homozygous variation (NM_001161352.1:c.1372C>T, p.Arg458Ter). The variant was not observed in publicly available or in-house databases. Immunofluorescent staining revealed that novel variant is not interfering with the synthesis of KCNMA1 however mutation exhibit dominant-negative effect on cell viability when compared to wild-type. 293T and MCF7 cells transfected with homozygous p.Arg458Ter mutation showed markedly increased KCNMA1 currents compared to controls on patch-clamp recording, and these data support loss-of-function effect of all KCNMA1 mutants.Conclusions: Herein we report a 15-year old boy who has neurological conditions. A novel homozygous stop-gain mutation detected by WES and confirmed by conventional sequencing. Afterward, functional characterization was conducted using two step-approach, immunostaining to detect subcellular effect of the variation and patch-clamp to detect a difference between mutant vs. wild-type of the protein. Homozygous mutation was considered as causative for this clinical condition. This study was supported by Bezmialem Vakif University, Scientific Research Projects Unit, Project No:2.2019/7.
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    A comparative study of parathyroid transport solution and university of Wisconsin solution: effect on calcium-sensing receptor and vitamin-D receptor during cold ischemia in parathyroid tissue
    (2019-09-16T00:00:00Z) Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; AKBAŞ, FAHRİ; KAZANCIOĞLU, RÜMEYZA; ERSOY, YELIZ EMINE
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    Paratiroit Nakli İmmünolojisi
    (2019-04-01T00:00:00Z) Ayşan, Mustafa Erhan; Göncü, Beyza Servet; Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH
    Paratiroit nakli, kalıcı hipoparatiroidinin tek tedavi seçeneğidir. Diğer organ nakillerinde olduğu gibi greft fonksiyonunun artırılması için alıcı-verici uyumu paratiroit nakli için de çok önemlidir. Paratiroit nakli (bazı özel vakalar dışında) doğası gereği immünsupresyonsuz olarak gerçekleştirilmek zorunda olduğu için başarılı nakiller yapmak oldukça güçtür. Bu güçlüğü aşabilmek için elimizde güvenilir parametreler olmalıdır. İlk paratiroit naklinin yapılmasından günümüze kadar geçen yaklaşık 100 yıl içinde ne yazık ki A, B, 0 uyumu dışında üzerinde konsensus sağlanan bir kriter yoktur. Literatürde bu konudaki bilgi miktarı yetersiz olduğu gibi üstelik var olan bilgilerin doğruluğu da şüphelidir. Konu üzerinde daha fazla çalışma yapılmalıdır. Bu gerçekleşene kadar paratiroitte immünsüpresyon yapılmaksızın yüksek başarı oranıyla ve uzun greft fonksiyonu gösteren allo-nakiller yapmak zor görünmektedir.
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    Minor histocompatibility antigen sp110: an evolutionary trace for parathyroid tissue
    (2019-09-15T00:00:00Z) Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; AKBAŞ, FAHRİ; ERSOY, YELIZ EMINE
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    Investigating differential miRNA expression profiling using serum and urine specimens for detecting potential biomarker for early prostate cancer diagnosis
    (2021-02-08T00:00:00Z) Hasanoğlu, Sevde; Göncü, Beyza Servet; Yücesan, Emrah; Atasoy, Sezen; Kayali, Yunus; Özten Kandaş, Nur; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; ATASOY, SEZEN
    Background/aim: MicroRNAs (miRNAs) are known up-to-date candidate biomarkers for several diseases. In addition, obtaining miRNA from different body fluids such as serum, plasma, saliva, and urine is relatively easy to handle. Herein we aimed to detect miRNAs as biomarkers for early stage prostate cancer (PC). For this purpose, we used urine and serum samples to detect any significant differences in miRNA profiles between patients and healthy controls. Materials and methods: Total ribonucleic acid (RNA) in urine and serum samples were isolated from eight untreated PC patients, thirty healthy individuals were screened for miRNA profile, and candidate miRNAs were validated. Whole urinary and serum miRNA profile was analyzed using Affymetrix GeneChip miRNA 4.0 Arrays. Candidate miRNAs were investigated by stem-loop reverse transcription- polymerase chain reaction. Results: When we analyzed the urinary samples of PC patients, 49 miRNAs were detected to be upregulated and 14 miRNAs were found to be downregulated when compared with healthy controls. According to the serum samples, 19 miRNAs were found to be upregulated, and 21 miRNAs were found to be downregulated when compared with healthy individuals as well. Interestingly, we detected only four overlapping miRNAs (MIR320A, MIR4535, MIR4706, MIR6750) that commonly increase or decrease in both serum and urine samples. Among them, MIR320A was found to be downregulated, and MIR4535, MIR4706, and MIR6750 were found to be upregulated for urine samples. However, only MIR6750 was upregulated and the other three miRNAs were downregulated for serum samples. Conclusion: Notably, the expression profile of MIR320A was significantly altered in urine specimens of prostate cancer patients. We considered that MIR320A has been evaluated as a valuable biomarker that can be used in the early diagnosis of PC.
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    Paratiroit Hücrelerinin Mikroenkapsülasyonu ve Nakil Yöntemi
    (2018-11-29T00:00:00Z) Yücesan, Emrah; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH
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    Hücre Mikroenkapsülasyonunda Manuel ve Kapsülasyon Sisteminin Hücre İzolasyon Tipi ve Aljinat Yüzdesine Bağlı Verimliliğinin Karşılaştırılması
    (2021-08-01T00:00:00Z) Düzenli, Ömer Faruk; Göncü, Beyza Servet; Selepcioğlu, Harika; Yücesan, Emrah; Ersoy, Yeliz Emine; Akçakaya, Adem; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; ERSOY, YELIZ EMINE; AKÇAKAYA, ADEM
    Amaç: Birçok endüstriyel sektörde kullanılan polimer malzemeler sağlık bilimlerinde de farklı işlemlerde kullanılmaktadır. Bu işlemlerden biri olan enkapsülasyon sistemi hücre nakli gibi terapötik uygulamalarda tercih edilmektedir. Enkapsülasyon çalışmalarında uygulanacak yaklaşıma göre kapsül yapısında kullanılacak polimer malzeme ve oluşan kapsül boyutu değişmektedir. Aljinat, kahverengi alglerden elde edilen, içeriğindeki farklı polimerik blok oranlarına bağlı olarak değişiklik gösteren doğal polimerlerden biridir. Bu çalışmada, kapsülasyon aşaması için kullanılacak olan değişik aljinat yüzdeleri uygulanarak paratiroid hücrelerinde ideal mikroenkapsülasyon prosedürlerinin belirlenmesi amaçlanmaktadır.Gereç ve Yöntem: Çalışmada sekonder hiperparatiroidi hastasından alınan bir adet paratiroid hiperplazi dokusundan mekanik ve enzimatik izolasyon yöntemleriyle hücre eldesi gerçekleştirilmiştir. İki farklı aljinat yüzdesi kullanarak hem manuel olarak hem de kapsülasyon cihazında otomatize olarak iki farklı akış hızı değerlendirilmiştir. Mikroenkapsüle edilen hücreler 64-79 gün boyunca in vitro olarak parathormon miktarları ölçülerek takip edilmiştir.Bulgular: Değerlendirilen aljinat yüzdelerinden %2’lik konsantrasyona sahip mikroenkapsüllerin oluşturulmasında kapsülasyon cihazında kullanılan 2 mL/dk akış hızıyla morfolojik stabilite gözlenmiştir. Ayrıca parathormon salınımı açısından hücre izolasyon tipi ve aljinat yüzdeleri arasında benzer sonuçlar elde edilmiştir.Sonuç: Uzun süreli mikroenkapsülasyon verimliliğinin arttırılması için yapısal ve fonksiyonel açıdan birçok parametrenin belirlenmesi gerekmektedir. Bu çalışma ile enzimatik izolasyon metoduyla elde edilen paratiroid hücrelerinin kapsülasyon sistemi kullanılarak artan akış hızında daha stabil bir yapı oluşturdukları belirlenmiştir.