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Now showing 1 - 6 of 6
  • Publication
    Simultaneous determination of hydrochlorothiazide, amlodipine, and telmisartan with spectrophotometric and HPLC green chemistry applications.
    (2022-06-03T00:00:00Z) Tiris, GİZEM; Mehmandoust, Mohammad; Lotfy, Hayam M; Erk, Nevin; Joo, Sang-Woo; Dragoi, Elena-Niculina; Vasseghian, Yasser; TIRIS, GİZEM
  • Publication
    High Performance Liquid Chromatographic Method For The Determination Of Amlodipine And Perindopril
    (2019-12-23T00:00:00Z) Lotfy, Hayam M.; Tırıs, Gizem; Erk, Nevin; TIRIS, GİZEM
    In the currently work; rapid, sensitive and validated high performance liquidchromatography (HPLC) method for the simultaneous determination of Amlodipin (ADP) andPerindopril (PER) in pure forms and pharmaceutical products were presented. Amlodipine(ADP), 3-O-ethyl 5-O-methyl 2-(2-aminoethoxymethyl)-4-(2-chlorophenyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate is a vasoselective dihydropyridine calcium antagonist, hasa pharmacokinetic profile that sets it apart from other calcium antagonists (1). Perindopril(PER) (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid is an angiotensin-converting enzyme inhibitor thatis used in the treatment of hypertension and heart failure. It is also used to reduce the risk ofcardiovascular events in patients with stable ischemic heart disease (2).Analysis was performed on the Waters Spherisorb® ODS-2 (5μm 150x4.6 mm) column.Mobile phase system has been consisted of acetonitrile : methanol : posphate buffer (65:5:30v/v/v). Specimen was enjected to column at 20 μl. Detection wavelength of 220 nm waspreferred for both compounds and flow rate was 1,5 ml/min. Analysis was completed 5minutes. Column oven temperature was adjusted 30 ºC. The retention time was observed at1.58 and 2.40 min for PER and ADP. Calibration graphs were constructed in the 10- 40μg/ml, 5-40 μg/ml concentration of per, adp respectively. The proposed method wasemployed with a high degree of precision and accuracy in the determination of all activeingredients. The method was suitable for the simultaneous determination and it can be appliedin the quality control department in industries, bio-pharmaceutics and bio-equivalence studies.
  • Publication
    Analytical tools for greenness assessment of chromatographic approaches: Application to pharmaceutical combinations of Indapamide, Perindopril and Amlodipine
    (2020-12-01T00:00:00Z) Saleh, Sarah S.; Lotfy, Hayam M.; Tiris, GİZEM; Erk, Nevin; Rostom, Yasmin; TIRIS, GİZEM
    The green profile of the proposed method was assessed and compared with reported classical methods via four tools of greenness which are: Eco-Scale, National Environmental Methods Index (NEMI), Assessment of Green Profile (AGP) and Green Analytical Procedure Index (GAPI). A simple, precise and rapid (RP-HPLC) has been developed for the quantification of Indapamide (IND), perindopril (PER) arginine and amlodipine besylate (ADB) in binary and ternary mixtures. A greener alternate RP-HPLC method was presented for the determination of pharmaceutical combinations composed of the cited medications using an eco-friendly eluent and quick run time with the least waste yield. Chromatographic separation was achieved using Waters Spherisorb ODS-2 C18 column (150 x 4.6 mm, i.d., 5 mu m) and eluent formed of acetonitrile : phosphate buffer (20 mM, pH = 3): methanol in the ratio of (65: 30: 5, by volume) at flow rate 1.0 mL/min with DAD-detection at 220 nm. This procedure was valid over linearity ranges of 0.5-20 mu g/mL, 2.5-40 mu g/mL and 2-40 mu g/mL for IND, PER and AML, respectively. The presented chromatographic method was completely validated regarding ICH guidelines and were statistically compared with those of the reported methods applying t-test and F-test at 95% confidence. The proposed method was found to be greener in terms of usage of hazardous chemicals and solvents, energy consumption, and waste production.
  • Publication
    (2021-06-25T00:00:00Z) Mehmandoust, Mohammed; Erk, Nevin; Tırıs, Gizem; TIRIS, GİZEM
  • Publication
    Electrochemical Determination of Sunset Yellow in river water and some food samples
    (2022-09-08T00:00:00Z) Tırıs, Gizem; Oven, Elifnaz; Mehmandoust, Mohammad; Erk, Nevin; Soylak, Mustafa; TIRIS, GİZEM