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DEMİREL, METİN

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METİN
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DEMİREL
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Now showing 1 - 10 of 15
  • PublicationMetadata only
    Molecular cloning and characterization of NAD(+) dependent isocitrate dehydrogenase enzyme from Shewanella putrefaciens
    (2021-10-01T00:00:00Z) Fahri, Akbas; Metin, Demirel; Ahmet, Ozaydin; Sahabettin, ŞAHBETTİN; AKBAŞ, FAHRİ; DEMİREL, METİN; SELEK, ŞAHABETTİN
    Isocitrate dehydrogenase (IDH) is a fundamental enzyme for carbon metabolism in the Krebs cycle. This enzyme is required for oxidation-reduction reactions in both eukaryotic and prokaryotic cells and plays a critical role in their growth and pathogenesis. In this study, we cloned the gene encoding NAD(+) dependent isocitrate dehydrogenase from Shewanella putrefaciens. The expression of recombinant protein was induced with 0.5 mM of IPTG. His-tagged IDH overexpressed in E. coli was purified and characterized. The expressed IDH enzyme was purified in an active soluble form. The molecular weight of the enzyme was confirmed with Western blotting. High sequence homology was observed with IDH sequences of other Shewanella strains and remarkable sequence homology was found with other bacteria reported in the database.
  • PublicationMetadata only
    G6PD AND GAPDH AS POTENTIAL DIAGNOSTIC BIOMARKERS FOR IRON DEFICIENCY ANEMIA
    (2021-10-21T00:00:00Z) Demirel, Metin; Selek, Şahabettin; Gül, Ayşe Zehra; Köktaşoğlu, Fatmanur; Sarıkaya, Ufuk; Yıldız, Tuğçe; Dulun Ağaç, Halime; DEMİREL, METİN; SELEK, ŞAHABETTİN; GÜL, AYŞE ZEHRA; KÖKTAŞOĞLU, FATMANUR; SARIKAYA, UFUK; YILDIZ, TUĞÇE; DULUN AĞAÇ, HALİME
  • PublicationMetadata only
    SERUM REFERENCE INTERVALS OF ZINC AND COPPER IN HEALTHY SUBJECTS OF TURKEY POPULATION
    (2021-10-21T00:00:00Z) Demirel, Metin; Selek, Şahabettin; Yıldız, Tuğçe; Sönmez, Rana; Gül, Ayşe Zehra; Sarıkaya, Ufuk; Köktaşoğlu, Fatmanur; DEMİREL, METİN; SELEK, ŞAHABETTİN; YILDIZ, TUĞÇE; GÜL, AYŞE ZEHRA; SARIKAYA, UFUK; KÖKTAŞOĞLU, FATMANUR
  • PublicationOpen Access
    Could the PON1 phenotype play a key role in insulin resistance?
    (2022-06-01T00:00:00Z) Sarikaya, Ufuk; Meydan, Sedat; Selek, Şahabettin; Sarikaya, Alime; Demirel, Metin; Gül, Ayşe Zehra; Yildiz, Tuğçe; SARIKAYA, UFUK; MEYDAN, SEDAT; SELEK, ŞAHABETTİN; DEMİREL, METİN; GÜL, AYŞE ZEHRA; YILDIZ, TUĞÇE
    Aim/objectivesRecent studies have shown that Paraoxonase (PON1) enzyme plays a possible role in insulin synthesis by stimulating insulin release from β-cells of the pancreas as well as its anti-atherosclerotic property. In our study, we revealed the relationship between phenotypes of the PON1 enzyme and insulin resistance (IR) and impaired fasting glucose (IFG).Materials and methodsA cohort of 71 IR, 63 IFG, and 68 healthy individuals was examined in this study. The phenotypic distribution was demonstrated by studying PON1 enzyme’s Paraoxonase (POase) and Arylesterase (AREase) activity with automated measurement kits.ResultsBy measuring the ratio of POase activity to AREase activity, 3 different phenotypes (QQ (Risky or Bad Phenotype), QR (Notre Phenotype), and RR (Good Phenotype)) were discovered. The results showed that IR and IFG individuals had riskier phenotypes compared to the control group. In addition, individuals with bad phenotypes were found to be 1.85 and 2.16 times more likely to get IR and IFG, respectively. Both groups were found to be four times more likely to be affected by the bad phenotype (odds ratio: 3.69 and 4.47 respectively).ConclusionIn this present study, the relationship between PON1 enzyme phenotypes and IR was evaluated for the first time in this field. Decreased PON1 activity and poor phenotype may also increase the development of hyperglycemia or diabetes mellitus (DM) due to IR and IFG. It may also predispose to diseases such as atherosclerosis. Therefore, we think that further investigations to explain the possible mechanisms underlying the relationship between PON1 phenotypes, IR and IFG will be useful in the early diagnosis and prevention ofprediabetes.
  • PublicationOpen Access
    Mass spectrometry-based untargeted metabolomics study of non-obese individuals with non-alcoholic fatty liver disease
    (2023-06-01) Demirel M.; Köktaşoğlu F.; Özkan E.; Dulun Ağaç H.; Gül A. Z.; Sharifov R.; Sarıkaya U.; Başaranoğlu M.; Selek Ş.; DEMİREL, METİN; KÖKTAŞOĞLU, FATMANUR; DULUN AĞAÇ, HALİME; GÜL, AYŞE ZEHRA; SARIKAYA, UFUK; BAŞARANOĞLU, METİN; SELEK, ŞAHABETTİN
    Objectives: Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by the accumulation of excessive fat in the liver, which can lead to fibrosis and has an increasing prevalence. NAFLD requires non-invasive diagnostic biomarkers. While typically observed in overweight individuals, it can also occur in non-obese/non-overweight individuals. Comparative studies on non-obese NAFLD patients are scarce. This study aimed to conduct a using liquid chromatography-high resolution mass spectrometry (LC-MS/MS)-based metabolic profiling of non-obese NAFLD patients and healthy controls. Materials and methods: The patient group consisted of 27 individuals with NAFLD, while the healthy control group included 39 individuals. Both groups were between 18 and 40 years old, had a BMI of less than 25 and had alcohol consumption less than 20 g/week for men and 10 g/week for women. Serum samples were collected and analyzed using LC-MS/MS. The data were analyzed using the TidyMass and MetaboAnalyst. Results: The LC-MS/MS analyses detected significant changes in D-amino acid metabolism, vitamin B6 metabolism, apoptosis, mTOR signaling pathway, lysine degradation, and phenylalanine metabolism pathways in non-obese NAFLD patients. Significant changes were also observed in the metabolites D-pantothenic acid, hypoxanthine, citric acid, citramalic acid, L-phenylalanine, glutamine, and histamine-trifluoromethyl-toluidide, β-hydroxymyristic acid, DL-Lactic acid, and 3-methyl-2-oxopentanoic. Overall, the study provides valuable insights into the metabolic changes associated with non-obese NAFLD patients and can contribute to the development of non-invasive diagnostic biomarkers for NAFLD.
  • PublicationMetadata only
    K VİTAMİNİ ÖLÇÜM METODUNUN GELİŞTİRİLMESİ VE REFERANS ARALIĞININ BELİRLENMESİ
    (2022-10-26) Demirel M.; Selek Ş.; DEMİREL, METİN; SELEK, ŞAHABETTİN
  • PublicationMetadata only
    METABOLIC PROFILING STUDY IN SERUM OF PATIENTS WITH POLYCYSTIC KIDNEY DISEASE AND CHRONIC KIDNEYDISEASE BASED ON 1 H NMR SPECTROSCOPY
    (2021-10-11T00:00:00Z) Köktaşoğlu, Fatmanur; Demirel, Metin; Gül, Ayşe Zehra; Elçioğlu, Ömer Celal; Aris, Özlem; Selek, Şahabettin; KÖKTAŞOĞLU, FATMANUR; DEMİREL, METİN; GÜL, AYŞE ZEHRA; ELÇİOĞLU, ÖMER CELAL; SELEK, ŞAHABETTİN
  • PublicationMetadata only
    Maternal Serum Levels of Zinc, Copper, and Thiols in Preeclampsia Patients: a Case-Control Study.
    (2021-03-11T00:00:00Z) Gul, Ayse Zehra; Atakul, Nil; Selek, ŞAHBETTİN; Sarıkaya, Ufuk; Yıldız, Tugce; Demirel, Metin; GÜL, AYŞE ZEHRA; SELEK, ŞAHABETTİN; SARIKAYA, UFUK; YILDIZ, TUĞÇE; DEMİREL, METİN
  • PublicationMetadata only
    Fumaraz enziminin Shewanella putrefaciens bakterisinden üretimi ve biyokimyasal tanı kitlerinde kullanılması
    (2021-10-31T00:00:00Z) Toruntay, Ceyhun; Korkmaz, Nur Damla; Selek, Şahabettin; Gül, Ayşe Zehra; Demirel, Metin; Sarıkaya, Ufuk; Akbaş, Fahri; TORUNTAY, CEYHUN; KORKMAZ, NUR DAMLA; SELEK, ŞAHABETTİN; GÜL, AYŞE ZEHRA; DEMİREL, METİN; AKBAŞ, FAHRİ