Finansman Kuruluşları

Permanent URI for this community

https://hdl.handle.net/20.500.12645/30258

Browse

Now showing 1 - 20 of 340

Metadata only
A clinical variant in SCN1A inherited from a mosaic father cosegregates with a novel variant to cause Dravet syndrome in a consanguineous family
(2015-07-01) Tuncer, FN; Altıokka uzun , G; Gormez, Z; Calik , M; Yuksel, B; Sagiroglu, MS; Iscan, AKIN; Yuceturk , B; Ugur Iseri, SA; Bebek, N; Baykan, B; Ozbek , U; İŞCAN, AKIN
A consanguineous family from Turkey having two children with intellectual disability exhibiting myoclonic, febrile and other generalized seizures was recruited to identify the genetic origin of these phenotypes. A combined approach of SNP genotyping and exome sequencing was employed both to screen genes associated with Dravet syndrome and to detect homozygous variants. Analysis of exome data was extended further to identify compound heterozygosity. Herein, we report identification of two paternally inherited genetic variants in SCN1A (rs121917918; p.R101Q and p.I1576T), one of which was previously implicated in Dravet syndrome. Interestingly, the previously reported clinical variant (rs121917918; p.R101Q) displayed mosaicisnn in the blood and saliva of the father. The study supported the genetic diagnosis of affected children as Dravet syndrome possibly due to the combined effect of one clinically associated (rs121917918; p.R101Q) and one novel (p.I1576T) variants in SCN1A gene. This finding is important given that heterozygous variants may be overlooked in standard exome scans of consanguineous families. Thus, we are presenting an interesting example, where the inheritance of the condition may be misinterpreted as recessive and identical by descent due to consanguinity and mosaicism in one of the parents. (C) 2015 Elsevier B.V. All rights reserved.
Open Access
A Comprehensive Review: Molecular and Genetic Background of Indirect Inguinal Hernias
(2021-04-01T00:00:00Z) SOMUNCU, SALİH; Somuncu, Ozge Sezin; SOMUNCU, SALİH
Background: The occurrence of indirect inguinal hernias (IIH) is 5 times more prevalent than that of direct inguinal hernias (IH) and it is 7 times more common in males, owing to the attendance of the processus vaginalis (PV) throughout testicular descent. Summary: In children, the immense mainstream of IH is indirect. The progress of IIH development in children is instigated with a patent PV, which is mostly treated by simple herniorrhaphy. Syndromes of the collagen, microfibril, elastin, and glycosaminoglycan constituents of the extracellular matrix may attend to the development of IH. Our recent research showed that the lack of epithelial-mesenchymal transition (EMT) in children contributes to the development of IIH, while the scenario is defined as the opposite in adults. However, there is still a lack of knowledge on all of the genetic and molecular causes of the disease. Key Messages: Here we aimed to review the published genetic background of IH, the deficiencies of connective tissue causing the disease, recently defined molecular pathways involved including EMT, and possible recurrence reasons. This comprehensive study can deliver an analytic outline aiding to define patients with IH combined with fundamental genetic diseases.
Metadata only
A Coumarin Bearing Amphiphilic Block Copolymer: Synthesis, Characterization and Micellization
(2016-07-21) TEMEL, BİNNUR; TEMEL, BİNNUR
Metadata only
A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum
(2020-02-01T00:00:00Z) Dincel, Demet; Topçu, Gülaçtı; DİNCEL, DEMET; TOPÇU, GÜLAÇTI
Metadata only
A new dinuclear copper (II) complex of 2,5-Furandicarboxyclic acid with 4(5)-Methylimidazole as a high potential alpha-glucosidase inhibitor: Synthesis, Crystal structure, Cytotoxicity study, and TD/DFT calculations
(1.03.2019) Avci, Davut; Alturk, Sumeyye; Sonmez, Fatih; Tamer, Omer; Basoglu, Adil; Atalay, Yusuf; Zengin Kurt, Belma; Ozturk, Dilek; Dege, Necmi
A new dinuclear copper (II) complex of 2,5-furandicarboxyclic acid with 4(5)-methylimidazole, [Cu (FDCA)((4(5)MeI)(2)](2)center dot 2H(2)O, was synthesized, and its structure characterized by XRD, FT-IR and UV-Vis spectroscopic techniques. The alpha-glucosidase inhibition and cytotoxicity study of the synthesized Cu (II) complex were determined by IC50 values. The optimized geometry and vibrational harmonic frequencies for the Cu (II) complex were obtained by using Density Functional Theory (DFT) of HSEh1PBE/6-311++G(d,p)/LanL2DZ level. TD-DFT/HSEh1PBE/6-311++G(d,p)/LanL2DZ level with CPCM model was applied to examine the electronic spectral properties and major contributions were determined via Swizard program. To investigate linear and nonlinear optical behavior of the synthesized Cu (II) complex, the alpha, Delta alpha and chi((1))/beta, gamma and chi((3)) parameters called linear/nonlinear optical parameters in gas phase and ethanol solvent were computed at the same level and basis set. Furthermore, molecular electrostatic potential (MEP) surface was determined by using the same level. The docking study of the Cu (II) complex to the binding site of the target protein (the template structure S. cerevisiae isomaltase) is fulfilled. Natural bond orbital (NBO) analysis was used to investigate the hyperconjugative interactions, inter- and intra-molecular bonding and to determine coordination around Cu (II) ion. Finally, present work is the first remarkable scientific report of mixed-ligand (H(2)FDCA and 4(5)MeI) Cu (II) complex as novel drug candidate for DM II. It is also determined that microscopic third-NLO parameters for the Cu (II) complex is remarkable.
Metadata only
A new dinuclear copper (II) complex of 2,5– Furandicarboxyclic acid with 4(5)-Methylimidazole as a high potential ?-glucosidase inhibitor: Synthesis, Crystal structure, Cytotoxicity study, and TD/DFT calculations
(2019-01-01) Sönmez, Fatih; Basoglu, Adil; Altürk, Sümeyye; Avcı, Davut; Tamer, Ömer; Öztürk, Dilek; Dege, Necmi; Zengin Kurt, Belma; Atalay, Yusuf; ZENGİN KURT, BELMA; ÖZTÜRK CİVELEK, DİLEK
Metadata only
A new method that can be used to overcome the condensation risks in radiant cooling systems and thermal comfort examinations
(1.01.2018) Koca, Aliihsan
In this study, we propose a novel cooling dehumidifying strategy, in which a condensing panel can be hydronically connected in series with the radiant cooling system to overcome condensation risk and improve indoor thermal comfort level. Based on the concept, the sensible heat-load is primarily covered by radiant cooling panels and some amount of latent heat and sensible heat can be treated by the condensing panel, while improving indoor thermal comfort level. Since, the surface temperature of the condensing panel is lower than the dew point temperature, while the radiant surface is higher for same supply water temperatures - condensation occurs only over the condensing plate. This paper evaluates the thermal comfort performance of the proposed novel condensing panel. For these aim, a new experimental chamber was developed. General thermal comfort level and the temperature, relative humidity ratio distribution in the tested room were evaluated for 10 different experimental cases. Multiple tests were conducted by varying surface temperature of the condensing panel and initial relative humidity ratios of air in the room. The results show that proposed simple solution can improve thermal comfort level, for the places where the latent heat loads are not considerable high, and reduce the condensation risk over the radiant cooling panels.
Metadata only
A New Mini-External Fixator for Treating Hallux Valgus: A Preclinical, Biomechanical Study
(2016-01-01T00:00:00Z) Ceylan, Hasan Huseyin; Sünbüloğlu, Emin; Bozdağ, Süreyya Ergün; KARA, DENİZ; POLAT, Gökhan; Erdil, Mehmet
Proximal metatarsal osteotomy is the most effective technique for correcting hallux valgus deformities, especially in metatarsus primus varus. However, these surgeries are technically demanding and prone to complications, such as nonunion, implant failure, and unexpected extension of the osteotomy to the tarsometatarsal joint. In a preclinical study, we evaluated the biomechanical properties of the fixator and compared it with compression screws for treating hallux valgus with a proximal metatarsal osteotomy. Of 18 metatarsal composite bone models proximally osteotomized, 9 were fixed with a headless compression screw and 9 with the mini-external fixator. A dorsal angulation of 10 degrees and displacement of 10 mm were defined as the failure threshold values. Construct stiffness and the amount of interfragmentary angulation were calculated at various load cycles. All screw models failed before completing 1000 load cycles. In the fixator group, only 2 of 9 models (22.2%) failed before 1000 cycles, both between the 600th and 700th load cycles. The stability of fixation differed significantly between the groups (p < .001). The stability provided by the mini-external fixator was superior to that of compression screw fixation. Additional testing of the fixator is indicated. (C) 2016 by the American College of Foot and Ankle Surgeons. All rights reserved.
Metadata only
A New Validated HPLC/UV Method for the Simultaneous Determination of Vildagliptin and Metformin in Pharmaceutical Preparations
(2013-08-16) KIZILTAS, M. V.; DİNCEL, DEMET; TEKKELİ, ŞERİFE EVRİM; TEKKELİ, ŞERİFE EVRİM; DİNCEL, DEMET
Metadata only
A novel mutation in LGI1 gene found in a series of consecutive epileptic patients with auditory aura.
(2015-06-07T00:00:00Z) Yücesan, Emrah
Metadata only
A novel series of mixed-ligand M(II) complexes containing 2,2′-bipyridyl as potent α-glucosidase inhibitor: synthesis, crystal structure, DFT calculations, and molecular docking
(2019-08-01) Avcı, Davut; Altürk, Sümeyye; Sönmez, Fatih; Tamer, Ömer; Başoğlu, Adil; Atalay, Yusuf; Zengin Kurt, Belma; Dege, Necmi; ZENGİN KURT, BELMA
Open Access
A novel stability-indicating method for determination of a new antidepressant effect of vortioxetine in a pharmaceutical formulation by using RP-HPLC
(1.12.2020) Tiris, Gizem; Alver, Cansu; Erk, Nevin
Background: A novel rapid, accurate, and stability-indicating reversed-phase high performance liquid chromatographic (RP-HPLC) and first derivative spectrophotometric determination were explained for the assay of vortioxetine (VRT) in bulk and pharmaceutical formulations. For RP-HPLC method, optimal separation and determination of VRT were achieved with a Waters Symmetry C-18, (100 x 4.6 mm, 3.5 mu m) analytical column using a mobile phase consisting of methanol:0.05 M potassium dihydrogen phosphate (pH:3.0 +/- 0.05) (30:70, v/v) in isocratic mode with flow rate of 1.3 mL min(-1). Injection volume was 20 mu L. The maximum absorption wavelength of VRT is 225.0 nm; hence, 225.0 nm was studied as the detection wavelength and column at 50 degrees C temperature. The caffeine was used as the internal standard (IS). On the other hand, the first derivative spectrophotometric method for the analysis of vortioxetine was performed by measuring the amplitude at 251.7 and 272.6 nm. Result: The HPLC method was found to be linear in the concentration ranges of 10.0-70.0 mu g mL(-1) with the coefficient value R-2 of 0.9998, and the mean recovery value was 100.7%. Further stability studies were done through exposure of the analyte solutions to various stress conditions: acid, alkali hydrolysis, chemical oxidation, and exposure to UV radiation. For the first derivative spectrophotometric method, linearity was observed in the concentration range 6.0-30.0 mu g mL(-1) (for 237.7 nm R-2 = 0.9999 and for 257.2 nm R-2 = 0.9997). Conclusion: The methods were validated in accordance with ICH guidelines with respect to linearity, accuracy, specificity, limit of detection, precision, and limit of quantification.
Metadata only
A potential therapeutic role in multiple sclerosis for stigmast-5,22-dien-3β-ol myristate isolated from Capparis ovata
(2017-07-01) ACAR, Özden Özgür; GAZİOĞLU, IŞIL; KOLAK, UFUK; ŞEN, ALAATTİN; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI
Open Access
A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
(2015-03-18) Elibol-Can, BİRSEN; Kilic, Ulkan; Gok, Ozlem; DUNDAR, TOLGA TURAN; DUNDAROZ, Mehmet Rusen; Torun, EMEL; Erenberk, UFUK; Uysal, Omer; ERENBERK, UFUK; TORUN, EMEL; ELİBOL, BİRSEN; UYSAL, ÖMER; DÜNDAR, TOLGA TURAN
Aging is defined as the accumulation of progressive organ dysfunction. Controlling the rate of aging by clarifying the complex pathways has a significant clinical importance. Nowadays, sirtuins have become famous molecules for slowing aging and decreasing age-related disorders. In the present study, we analyzed the SIRT1 gene polymorphisms (rs7895833 A>G, rs7069102 C>G and rs2273773 C>T) and its relation with levels of SIRT1, eNOS, PON-1, cholesterol, TAS, TOS, and OSI to demonstrate the association between genetic variation in SIRT1 and phenotype at different ages in humans. We observed a significant increase in the SIRT1 level in older people and found a significant positive correlation between SIRT1 level and age in the overall studied population. The oldest people carrying AG genotypes for rs7895833 have the highest SIRT1 level suggesting an association between rs7895833 SNP and lifespan longevity. Older people have lower PON-1 levels than those of adults and children which may explain the high levels of SIRT1 protein as a compensatory mechanism for oxidative stress in the elderly. The eNOS protein level was significantly decreased in older people as compared to adults. There was no significant difference in the eNOS level between older people and children. The current study is the first to demonstrate age-related changes in SIRT1 levels in humans and it is important for a much better molecular understanding of the role of the longevity gene SIRT1 and its protein product in aging. It is also the first study presenting the association between SIRT1 expression in older people and rs7895833 in SIRT1 gene.
Metadata only
A skeletochronological study of age, growth and longevity in two freshwater turtles, Emys orbicularis and Mauremys rivulata from Mediterranean Turkey (Reptilia: Testudines).
(2016-02-01) Ayaz, Dinçer; Çiçek, Kerim; KUMAŞ, MELTEM; Tok, Cemal Varol; KUMAŞ, MELTEM
We estimated growth parameters of Emys orbicularis and Mauremys rivulata in Mediterranean Turkey with the skeletochronological method, using specimens drowned in fyke nets. In adult E. orbicularis, the median age was 8 years in males and 10 years in females. The median age of adult M. rivulata was 10 years for both sexes. Both species reach sexual maturity at an age of 5-7 years. No difference was found in age composition between the sexes.
Metadata only
Aflatoxins in various food from Istanbul, Turkey
(2013-12-01) Hacibekiroglu, IŞIL; Kolak, U.; GAZİOĞLU, IŞIL
The present work reports the total aflatoxin and aflatoxin B1 levels in 62 food samples from Istanbul, Turkey. The total aflatoxin content in dried American cucumber, squash, tomato, okra and saffron samples was found to be 1.7g/kg. AFB1 levels in five dried vegetables (red bell pepper, American cucumber, squash, tomato and okra), two tea (linden and jasmine flower) and three spice samples (cardamom, galangal and saffron) were 1g/kg. Of the tested samples, 76% exceeded legal limits of total aflatoxin. The highest levels were determined in chestnut (232.9g/kg), nutmeg (206.1g/kg) and sumac (182.5g/kg). These findings confirm the existing knowledge that food should be regularly and effectively controlled.
Metadata only
Albumin-polymer conjugate nanoparticles and their interactions with prostate cancer cells in 2D and 3D culture: comparison between PMMA and PCL
(2016-01-01) JIANG, Yanyan; Lu, Hongxu; Dag, AYDAN; HART-SMITH, Gene; STENZEL, Martina H.; DAĞ, AYDAN
Using proteins as the hydrophilic moiety can dramatically improve the biodegradability and biocompatibility of self-assembled amphiphilic nanoparticles in the field of nanomedicine. In this study, we fabricated and evaluated curcumin loaded albumin-polycaprolactone nanoparticles as a novel drug delivery system for prostate carcinoma therapeutics and compared their performance to poly(methyl methacrylate) (PMMA), a non-degradable and amorphous polymer. The maleimide functionalized poly(epsilon-caprolactone) (PCL) was obtain using ring opening polymerization (ROP) of epsilon-caprolactone where N-(2-hydroxyethyl) maleimide was used as an initiator. The resorbable albumin-polymer conjugate was prepared by conjugating the hydrophobic maleimide-terminated PCL to the hydrophilic bovine serum albumin (BSA) via a simple Michael addition reaction. PMMA was conjugated in a similar manner. The amphiphilic BSA-polymer conjugates can self-assemble into nanoparticles, displaying well-defined structure, prolonged storage stability, and excellent biocompatibility. The BSA nanoparticles, with encapsulated curcumin, exhibited highly enhanced antitumor activity compared to free curcumin. Furthermore, the high efficacy of the curcumin loaded nanoparticles was verified by effectively inhibiting the growth of three-dimensional LNCaP multicellular tumour spheroids. The cytotoxicity was attributed to the efficient cellular uptake of the nanoparticles through caveolic endocytosis. The direct comparison between PCL and the PMMA revealed that drug loading and release as well as cytotoxicity is not significantly affected by the nature of the polymer. However, it seems that nanoparticles based on PMMA penetrate quicker into LNCaP multicellular tumour spheroids thanks to the increased stability. The faster penetration was found to reduce the toxicity of the nanoparticles as evidenced by the lower number of dead cells. In contrast, the fully degradable PCL-based nanoparticles were more efficient in delivering the drug, thus limiting the growth of LNCaP multicellular tumour spheroids.
Metadata only
Ameliorative Effects of Testosterone Administration on Renal Redox Homeostasis in Naturally Aged Rats.
(2015-08-01T00:00:00Z) YANAR, K; ATUKEREN, P; CEBE, T; KUNBAZ, A; OZAN, T; KANSU, ABDULLAH; DURMAZ, S; GÜLEÇ, V; Belce, AHMET; AYDıN, S; ÇAKATAY, U; RIZVI, SI; KANSU, ABDULLAH; BELCE, AHMET
Metadata only
An ethnobotanical study of the useful and edible plants of İzmit
(2012-06-01T00:00:00Z) Kızılarslan Hançer, Çağla; Özhatay, Neriman
Metadata only
An ethnobotanical study of the useful and edible plants of Izmit
(2012-01-01T00:00:00Z) KIZILARSLAN HANÇER, ÇAĞLA; Ozhatay, Neriman
An ethnobotanical study was carried out in Izmit province (Northwest Turkey). During this study, 145 specimens were collected from the area. Informations about plants such as their usages and used parts were recorded. The stud y revealed that, 96 plant taxa belonging to 42 families had ethnobotanical usages in this area. Among these 96 plant taxa, 60 taxa (51 wild, 9 cultivated) were used as edibles and 64 taxa (51 wild, 13 cultivated) were used for different purposes. The results of our study show that even in countrys that are situated in close proximity to metropolitan cities, the ethnobotanical usages of plants are still alive.

Browse

Browsing Finansman Kuruluşları by Title

Results Per Page

Sort Options